Labshake search
Citations for Bio-Rad :
9101 - 9150 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
-
bioRxiv - Cell Biology 2023Quote: Proteins were resolved by SDS-PAGE using 4-20 % or 4-15% TRIS-glycine gradient midi gels (Bio-Rad, Hercules, CA, USA), in electrode buffer (0.1% w/v SDS ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein was diluted with Laemmli buffer and loaded without heat denaturation onto a Criterion 10-20% tris-HCl gel (Bio-Rad, 3450043) run in tris-glycine SDS buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
-
bioRxiv - Bioengineering 2023Quote: ... All samples with equal amounts of protein were loaded on gels and separated by reducing SDS-PAGE and transferred to PVDF membranes (Bio-Rad, US). Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2023Quote: ... the supernatant in a new tube received 20% glycerol and protein concentration was estimated by Bradford’s Dye Reagent Concentrate (BioRad Laboratories, Hercules, CA). For gel electrophoresis ...
-
bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 mg of whole cell extract or 40-80 mg of liver homogenate was loaded on Mini-Protean TGX protein gel (4-20% or 7.5%) (Bio-Rad, Hercules, CA) and transferred to Immobilon-P membranes by electrophoresis (MilliporeSigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... were loaded on 10 % or 4–20 % Mini-PROTEANR TGX™ Precast Protein Gels and separated at 100 V in Tris/Glycine/SDS buffer in Mini-Protean Tetra System (Bio-Rad). The proteins were transferred to a polyvinylidene difluoride membrane (PVDF ...
-
bioRxiv - Microbiology 2023Quote: ... extracellular proteins were separated on SDS 15% (m/v) polyacrylamide gels and electroblotted to Immun-Blot polyvinylidene difluoride membranes (Bio-Rad, USA) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: The interaction between nucleic acids (ligands) and the proteins (analytes) were measured by the surface plasmon resonance (SPR) technique using a ProteOn XPR36TM (Bio-Rad Laboratories). NLC Sensor chips precoated with NeutrAvidin (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein samples (15 μg/lane) were subjected to analysis by 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620264) using either semi-dry or wet electroblotting (for proteins with a molecular weight of 100 kDa and above) ...
-
bioRxiv - Neuroscience 2023Quote: ... We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad) at 25 V for 7 min ...
-
bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
-
bioRxiv - Immunology 2023Quote: ... a total of 10–20 µg protein was prepared and loaded onto 4 to 20% gradient Mini-PROTEAN TGX Gels (Bio-Rad, 4561093) and electrophoretically separated at 135 V ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Between 20-30 µg of protein was electrophoresed in 4-15% miniPROTEAN TGX Stain-Free pre-cast gels (BioRad, 4568084/4568085/4568086) using electrophoresis buffer [24.76 mM Tris Base (American Bio AB02000-05000) ...
-
bioRxiv - Cell Biology 2023Quote: Equal amounts of proteins were separated on 8% reducing SDS-PAGE and transferred to Nitrocellulose membranes (Ref# 162-0112, BIO-RAD, Germany). Primary antibodies anti-SERCA2a and anti-SERCA2b targeted the specific ends of SERCA2a and SERCA2b (see supplement) ...
-
bioRxiv - Neuroscience 2023Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25 V ...
-
bioRxiv - Microbiology 2023Quote: ... Gels were loaded with samples containing 10 μg total protein before electrophoresis on a 16.5% Tris Tricine gel (Bio-Rad, Hercules, CA). Gels were stained with the Pierce Silver Stain kit (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
-
bioRxiv - Developmental Biology 2024Quote: ... of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories). List of primary antibodies is provided in Supplementary Table 2 ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein was resolved using SDS-PAGE and transferred to the nitrocellulose membrane using a transfer system (Trans-Blot Turbo; Bio-Rad Laboratories). The membrane was blocked using 5% skimmed milk (American Bioanalytical ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Trans-Blot® Turbo™ Mini PVDF Transfer membranes with the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% skimmed milk in PBS with added Tween20 0.05% (Merck ...
-
bioRxiv - Microbiology 2024Quote: ... The blots were washed three times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad, #1705061) or SuperSignal West Femto (ThermoScientific ...
-
bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
-
bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed as before and protein bands visualised using the enhanced chemiluminescent substrate (ECL) with the ChemiDoc™ XRS system (BioRad, USA). Details of primary and secondary antibodies used are in Table 2.
-
bioRxiv - Neuroscience 2024Quote: ... The proteins were then transferred from the gel to a nitrocellulose membrane using the Trans-Blot Turbo™ Transfer System (Bio-Rad) at 25V ...
-
bioRxiv - Neuroscience 2024Quote: ... 30 µg of protein was loaded into each well of a 4-15% Stain-Free polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and resolved via SDS-PAGE ...
-
bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95°C and resolved by SDS-PAGE in 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (#4561086, BioRad). After electrophoresis ...
-
bioRxiv - Cancer Biology 2024Quote: ... A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color, #1610374, Bio-Rad, Hercules, CA, USA). Transfer on PVDF membranes was done using Pierce Western blot transfer buffer (#35040 ...
-
bioRxiv - Cell Biology 2024Quote: ... Then an equal amount of proteins were separated on a polyacrylamide gel with pre-cast gradient (Mini-PROTEAN® Precast Gels, acrylamide 4% −20%, Bio-Rad) and transferred to nitrocellulose membrane (Trans-Blot® Turbo ™ Midi Nitrocellulose Transfer Packs ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins from total and bound fractions were separated by 7.5 % SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). To reduce the background signal ...
-
bioRxiv - Plant Biology 2024Quote: ... The protein bands containing PSII supercomplexes were cut out and eluted by a Model 422 Electro-Eluter (Bio-Rad, Hercules, CA, USA). The relative amount of PSII supercomplexes were quantified by dot immunoblot analysis with D1 antibody (Agrisera ...
-
bioRxiv - Cancer Biology 2024Quote: ... Thirty micrograms of each protein sample were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Bio-Rad, 1704150EDU). Non-specific binding sites on the membranes were blocked with 5% BSA in TBS-Tween 0.1% for 60 min ...
-
The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Protein extracts were boiled for 10 minutes and then loaded on polyacrylamide gels (TGX Stain-Free FastCast gels (Bio-Rad, 1610181/1610183) or home-made gels ...
-
bioRxiv - Microbiology 2024Quote: ... 20 μg of total protein was loaded onto each lane of SDS-PAGE on a 10% acrylamide gel (BIO-RAD cat. #4561034) and run 45 min at 60 V followed by 45 min at 110 V ...
-
bioRxiv - Microbiology 2024Quote: ... 8 μL of lysate were loaded in each lane and resolved on a 7.5% Mini-PROTEAN® TGX™ precast protein gel (Biorad 4561025) for PBP1a and PBP1b samples ...
-
bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
-
bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
-
bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad] ...
-
bioRxiv - Cell Biology 2024Quote: ... After running the samples by an SDS polyacrylamide gradient gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and transferring them to a PVDF membrane (350mA/1h) ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
-
bioRxiv - Plant Biology 2024Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
-
bioRxiv - Immunology 2024Quote: ... A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked with fat-free milk powder (5% w/v ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 20 mg of total protein was separated by SDS-PAGE on polyacrylamide gels (10%) and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Inc.). The membrane was probed with antibodies against Flag (#F7425 ...
-
bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...