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Citations for Bio-Rad :
8851 - 8900 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Samples were heated at 95 °C for 5 minutes to denature and reduce the proteins and were then separated by electrophoresis through 4-15% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 µg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Mini TGX gels (10%, Bio-Rad, Hercules, USA) according to standard protocols and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 655 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S4 for raw Lowry assay data.
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 550 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S5 for raw BCA assay data.
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 µg protein were loaded on 12% SDS-PAGE gels and separated with a Mini protean III system (Bio-Rad). The proteins were transferred onto nitrocellulose membranes by electroblotting adopting standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal quantity of protein for the respective samples were resolved by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked with 5% nonfat dry milk ...
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bioRxiv - Physiology 2024Quote: ... 5 ug (BAT) or 10 ug (ingWAT) of protein was separated on a 12% polyacrylamide gel (Mini-PROTEAN® TGXTM, BioRad). Proteins were then transferred to a PVDF membrane (BioRad ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using the BioRad Mixed Molecular Weight Protein Transfer setting (7 min, 1.3 A, 25 V) on the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150) with Trans-Blot Turbo Transfer Buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo). The membrane was blocked with 5% non-fat dry milk in 1X TBST for 1 hour ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were separated by SDS-PAGE using 7.5% gels or Any kD Mini-PROTEAN TGX Precast Protein Gels (Biorad, cat. 4569033) and transferred to PVDF membranes using a Trans-Blot Turbo Transfer System (Biorad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
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bioRxiv - Immunology 2024Quote: The pellet can be saved as a source of LPS and the soluble protein antigens were treated overnight with Affi-Prep Polymyxin Resin (Bio-Rad) to remove trace amounts of LPS remaining.
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bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.
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bioRxiv - Cell Biology 2024Quote: ... The refolded protein was separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (NGC, Bio-Rad) to remove protein aggregates ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE for 4 minutes (or until all the sample had entered the gel) at 200V (two wells per sample; 4-20% Mini-PROTEAN TGX precast protein gel, BIO-RAD), then stained with Coomassie blue for ten minutes before being washed 4 X with ddH2O (10 minutes each ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Biochemistry 2024Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting based on the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Bioengineering 2024Quote: ... Gels were stained for proteins using Coomassie R-250 and subsequent gel images were captured on a Chemidoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled for 5 min and 20 to 30 μg of protein were separated by SDS–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked in 3% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as either Coomassie Blue staining (for the TGX Precast Protein Gel) or the ChemiDoc Touch Imaging System (Bio-Rad) (for the TGX Stain-Free™ Protein Gel).
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... ten micrograms of proteins were separated by SDS-PAGE and transferred onto 0.2 μm PVDF membranes (Bio-Rad Laboratories, Inc., 1704156) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from beads using 400 mM Imidazole and their concentrations were determined using a Bradford Assay (BioRad, Hercules, CA) with BSA standard ...
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bioRxiv - Plant Biology 2024Quote: ... Stain-free gels were activated and total protein on gel were detected and recorded using a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Plant Biology 2024Quote: ... Twenty-five μg of protein per well were analyzed in an SDS-PAGE using a Mini-PROTEAN Tetra electrophoresis system (Bio-Rad) and transferred to a Trans-Blot transfer nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Each gel was transferred to a methanol-activated Immobilon-FL PVDF membrane (MilliporeSigma) which was then UV-activated and imaged for total protein content on a Chemidoc MP (Bio-Rad). Membranes were blocked at room temperature for 1 hr in a 1:1 mixture of PBS and fluorescent blocking buffer (Rockland Immunochemicals ...
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bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Microbiology 2024Quote: ... Fractions with the protein of interest were concentrated and further subjected to anion exchange chromatography on a Resource Q (Bio-Rad) 6 ml-column to remove remaining contaminants with a NaCl salt gradient from 50 mM to 500 mM ...
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bioRxiv - Cell Biology 2024Quote: ... A total 20 μg protein per extract was boiled in 1X reducing sample buffer and used for SDS-PAGE (BioRad, 4561083). Validation of gene knockdowns was performed by Western blotting using primary antibodies against STARD7 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...