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Citations for Bio-Rad :
8701 - 8750 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Membranes were visualized by chemiluminescence and under epi-blue to visualize total protein No-Stain labeling using a ChemiDoc MP imaging system (Bio-Rad). Densitometric analysis was performed using ImageLab software (Bio-Rad).
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bioRxiv - Genetics 2022Quote: ... and 25µg of protein was loaded in each well and resolved on 4-15% gradient Tris-Glycine gel (Bio-Rad - 4561086). Semi-dry transfer was done onto 0.2µm Nitrocellulose membrane as per Trans-BlotTurbo Kit (Bio-Rad - 1704270 ...
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bioRxiv - Genetics 2022Quote: ... or 25 µg (mouse tissues) of proteins were loaded per lane on Any kD or 4-20% Criterion TGX stain-free precast gels (Bio-Rad) and transferred to Immobilon-FL PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... the displayed S-trimer protein bands on SDS-PAGE gels were scanned and quantified by ChemiDoc MP imaging system (Bio-Rad) and ImageJ.
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Microbiology 2022Quote: ... All radioactive protein samples were separated by size on a 4-to-20% Mini-Protean TGX precast SDS-PAGE gel (Bio-Rad). The gel was then dried and imaged using a Typhoon phosphorimager (Molecular Dynamics GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Microbiology 2022Quote: ... β-lactamase activities were normalized relative to total protein concentration measured using Pierce Coomassie Plus Assay Kit (Bio-Rad, CA, USA) to obtain the enzyme activity in units pr ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Twenty-five µg of protein from each sample was ran (100 V, 100 min) on polyacrylamide gels (Cat. No. 5671095, Bio-Rad) using tris-glycine SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (25 µg) were separated by electrophoresis on 4-15% mini-PROTEAN TGX precast gels (Bio-Rad, cat# 456-1083) and transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
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bioRxiv - Physiology 2022Quote: ... diluted with 5X Laemmli buffer and boiled for 5 minutes at 95°C before being subjected to SDS-polyacrylamide gel electrophoresis using 15% (made in-house) or 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Physiology 2022Quote: ... after which the separated protein was transferred to a polyvinylidene fluoride (PVDF) membrane in a Trans-Blot Turbo System (Bio-Rad) at 25 V (1.3 A ...
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bioRxiv - Physiology 2022Quote: 20 µg of protein were loaded and resolved on 10% TGX fast cast gels (cat#1610173, Bio-Rad, Hercules, California, USA) for 30 min at 250 V ...
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 60 μg of each protein sample was loaded on the polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membrane (TransBlot Turbo, Bio-Rad) with semi-dry transfer (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Western blot analysis was performed by loading 7.5 g of total protein per lane into 7.5% Tris-glycine SDS-PAGE gels (Bio-Rad, catalog #4561026) and transferring to methanol-activated PVDF membranes (Bio-rad ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
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bioRxiv - Bioengineering 2022Quote: ... SDS page was performed on the samples and the proteins were transferred to nitrocellulose membranes using wet transfer (Bio-Rad Laboratories). The membranes were blocked with PBS with 0.1% Tween-20 (PBST ...
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bioRxiv - Cell Biology 2022Quote: ... The same amount of proteins for each lysate was then separated by SDS-PAGE on 4-15 % precast gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Physiology 2024Quote: ... Protein obtained was run on SDS-PAGE on precast polyacrylamide gel (Mini-PROTEAN TGX, 4%-20%; Bio-Rad Laboratories, Hercules, CA) and transferred to a polyvinylidene difluoride membrane via a semi-wet transfer equipment (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2024Quote: ... They were resolved by SDS–PAGE using a Bio-rad system and 4–15% Mini-PROTEAN TGX Precast Protein Gels (#4561086, Bio-rad) and transferred to nitrocellulose membranes (pore size 0.45 µm ...
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bioRxiv - Synthetic Biology 2024Quote: ... the maximum amount of protein that could be loaded to keep protein mass constant across all samples on a gel) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: ... 5 ug (BAT) or 10 ug (ingWAT) of protein was separated on a 12% polyacrylamide gel (Mini-PROTEAN® TGXTM, BioRad). Proteins were then transferred to a PVDF membrane (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Molecular Biology 2024Quote: 10 µL of the normalized protein samples were loaded onto a Mini-PROTEAN TGX Any kD (Bio-Rad Catalog #456-9036) SDS-polyacrylamide gel and electrophoresed in 1X SDS-PAGE Running Buffer (25 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: Biotinylated proteins were eluted from streptavidin beads by the addition of 20 μL of elution buffer (2X Laemmli sample buffer [Bio-Rad] ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were eluted from beads by adding the loading buffer (4X Laemmli sample buffer [Bio-Rad] with 20 mM dithiothreitol) followed by a 10 min incubation at 95°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification of the relative protein expression in ΔPr-A9 cells compared to the WT cells was determined in Image Lab software (Bio-Rad). The average signal of duplicate spots subtracting background value from negative control spots represented the levels of each protein ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 µg protein were loaded on 12% SDS-PAGE gels and separated with a Mini protean III system (Bio-Rad). The proteins were transferred onto nitrocellulose membranes by electroblotting adopting standard protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal quantity of protein for the respective samples were resolved by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked with 5% nonfat dry milk ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µg of the membrane protein eluate fraction quantified by BSA were diluted to 32 μl in lysis buffer containing loading Laemmli buffer (Bio-Rad) supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: The proteins were separated on polyacrylamide gels and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were blocked with 5% NFDM in TBST (50 mM Tris/HCl pH 8 ...