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Citations for Bio-Rad :
8751 - 8800 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: Biotinylated proteins were eluted from streptavidin beads by the addition of 20 μL of elution buffer (2X Laemmli sample buffer [Bio-Rad] ...
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bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
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bioRxiv - Microbiology 2024Quote: ... Proteins of interest (FtsZ, FtsN and MinD) were transferred to nitrocellulose membranes using a semi-dry Transfer-Blot apparatus (Bio-Rad). The Nitrocellulose membranes were then blocked in 5% (w/v ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: 10 µL of the normalized protein samples were loaded onto a Mini-PROTEAN TGX Any kD (Bio-Rad Catalog #456-9036) SDS-polyacrylamide gel and electrophoresed in 1X SDS-PAGE Running Buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and 30 µg of total protein extract were loaded on 10% acrylamide-bis-acrylamide gels or 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex) ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (10–30 μg) were run on a 10% SDS–polyacrylamide gels and then transferred to a nitrocellulose membrane (Biorad®). The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μg aliquots of mitochondria were flash-frozen in liquid nitrogen after measuring the protein concentration by a Bradford assay (Bio-Rad) and stored at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... 40-80 μg protein was separated on SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (#162–0177; Bio-Rad). Membranes were blocked in phosphate buffered saline (PBS)-Tween with 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μg of total protein per sample was subjected to reducing and denaturing SDS-PAGE prior to Western transfer onto nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk in PBS containing 0.1% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad). Resolved proteins were transferred onto 0.2μM PVDF membrane by the Criterion TM Blotter (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... USA) and the relative optical density of protein bands was quantified using Quantity One Software from Bio-Rad (Hercules, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µg of the membrane protein eluate fraction quantified by BSA were diluted to 32 μl in lysis buffer containing loading Laemmli buffer (Bio-Rad) supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred from the unstained gel to polyvinylidene fluoride membranes using the Trans-Blot turbo transfer system (Bio-Rad Laboratories). An iBind™ Flex system (Invitrogen/ThermoFischer ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of each sample was separated on an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). Proteins were electrophoretically transferred to a PVDF membrane using a Trans-Blot® Turbo™ Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein were subjected to SDS-PAGE and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed and the labelled protein bands were visualized by using Pierce ECL reagent and a ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein extract was loaded into a 10% TGX Stain-Free FastCast Acrylamide Gel (Bio-Rad, Hercules, CA, USA) or SDS polyacrylamide 15% running gel in the case of histone analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and light units were normalized by the total protein concentrations of the corresponding WCEs determined using the Bradford reagent (BioRad, 5000006).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... Cyt-b5 and other protein band intensities were determined using the volume contour tool Image Lab (Bio-Rad; Hercules, CA, USA) or ImageJ software ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total protein samples (40-50 µg) were resolved by polyacrylamide gels using self-made or pre-cast gels (Bio-Rad). Proteins were electroblotted on nitrocellulose membranes (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... The subsequent protein pellet was solubilized in 50 µL Laemmli sample buffer and 20 µg protein was run in a 12.5% SDS-Page gel (BioRad Criterion system). The gels were fixed and stained with Coomassie blue (GelCode blue ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins (20 μg per lane) were separated by SDS–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked in 4% milk in TBST [0.01 M tris (pH7.4) ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Band intensities were normalized to total protein in the sample quantified within each lane using Stain-Free Dye Imaging (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: Purified protein samples were visualized to evaluate purity by SDS-PAGE using the Bio-Rad mini-protean system (Bio-Rad laboratories). Samples were mixed in a 1:1 ratio with 2x Laemmli sample buffer containing 50 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysate samples with the same amount of total protein (40-50 μg) were mixed with 4x Laemmli Sample Buffer (Bio-Rad #161-0737 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Bioengineering 2023Quote: ... 30μg of total protein from each sample was placed into Mini-PROTEAN (R symbol) TGX (™) 4-20% gels (Bio-Rad) and subjected to SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...