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Citations for Bio-Rad :
8051 - 8100 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad) in Tris-Glycine-SDS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Retinas were processed and cytokines protein levels were measured by BioPlex suspension array (Bio-Rad Life Science, Hercules, CA, USA) as previously described (21).
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins containing 5× SYPRO™ Orange were loaded onto Hard-Shell® 96-well PCR Plates (Bio-Rad) for measurement ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were separated by SDS-PAGE using a Mini-Protein Tera Cell system (Bio-Rad Laboratories, Inc., Hercules, USA, CA). Proteins were visualized by CBB staining as described elsewhere.
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bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Biochemistry 2020Quote: ... The extracted protein (1000 mg) was loaded onto the 17 cm IPG gel strips (nonlinear, pH 3.0-10.0, Bio-Rad, USA) according to Chen et al (14) ...
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bioRxiv - Biochemistry 2020Quote: ... Tissue lysates for Western blot were prepared as previously described [11] and sample protein content was determined by Bradford assay (BioRad). Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared of insoluble debris by centrifugation prior to determination of protein concentration by BCA assay (BioRad, CA, USA). Lysates were electrophoresed through either 4-12% NuPAGE or 8% E-PAGE gels (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were either imaged directly (for fluorescent proteins) or stained with Coomassie blue and then imaged using a ChemiDoc gel imager (BioRad). Gel images were analyzed using Fiji ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... Protein was transferred to a nitrocellulose membrane (Hybond ECL; Amersham Biosciences) using a semi-dry transfer system (Bio-Rad Laboratories) at 25mV for 45 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant of the bacterial lysate was purified by affinity chromatography using an automated Profinia Protein Purification system (Bio-Rad), followed by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Scientific until approximately 3 cm of separation was obtained between the 25 and 37 kDa protein standards (Bio-Rad; 1610375). Using electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biophysics 2021Quote: ... 5 µL of purified (mutant) protein sample was mixed with 1.67 µL of 4X Laemmli loading buffer (Bio-Rad 1610747), then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046 ...
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bioRxiv - Cell Biology 2021Quote: ... 25 µg of protein lysate was heat-denatured at 95°C for 5 minutes in Laemmli sample buffer (Bio-Rad), and then separated using a 4 to 12% Bis-Tris polyacrylamide gel (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Neuroscience 2020Quote: ... PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad). The PVDF membrane was blocked with 5 % bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Mini-PROTEAN TDX Precast Protein Gels and Trans-Blot Turbo RTA Mini PVDF Transfer Kit were purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded in a 10-well 4-20% Mini-PROTEAN TGX Precast protein gel (BIO-RAD, Catalog #4561094). The gel was run at 150V for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
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bioRxiv - Plant Biology 2020Quote: ... Relative protein levels of three to four biological replicates were quantified using Image Lab™ Software (Bio-Rad, www.bio-rad.com).
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bioRxiv - Microbiology 2020Quote: ... 5 μl of the supernatant was used to normalize the level of total protein in each sample via BCA assay (BioRad) and the supernatant was then flash frozen on dry ice ...
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bioRxiv - Plant Biology 2020Quote: ... separated proteins were transferred onto a membrane by using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad) and the TransBlot Turbo device (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... SDS-PAGE gels were run on a Protein III system and transfers were performed with the Trans-Blot Turbo Transfer System (BioRad). Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). After electrophoretic separation ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with Coomassie Brilliant Blue or proteins were transferred into nitrocellulose membranes in Trans-Blot-Turbo Transfer Buffer (BioRad) for 7 min in a Trans-Blot Turbo Transfer System (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... The equivalent volume to 10 μg of protein was run in a Stain-Free FastCast 12.5% polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo semi-dry system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated by protein electrophoresis at 150 V for 1h using 4-20% Mini-Protean TGX pre-cast gels (BioRad) and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Separated proteins were then electroblotted using a TransBlot Turbo system to TransBlot Turbo Mini-size PVDF membranes (Bio-Rad, 1704272). Membranes were subsequently blocked with 1x TBS containing 0.1% Tween 20 (1x TBST ...
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bioRxiv - Neuroscience 2019Quote: The amount of protein was prepared from homogenized tissues or tells and determined using the Bradford quantification assay (Bio-Rad). Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... 20 µL of each sample was loaded and the proteins were resolved using 10% SDS-PAGE resolving gel followed by transfer to a polyvinylidene difluoride membrane (BioRad) at 100 mA for 12 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes using either a Trans-Blot® Turbo™ Transfer System (7 minutes, 25V, BioRad) or an iBlot™ 2 Dry Blotting System (P0 method ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad) per the manufacturer’s instructions and blocked with 100 mg/mL skim milk in 1x TBS/Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini-PROTEAN® electrophoresis unit (BioRad, Hercules, CA) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
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bioRxiv - Microbiology 2021Quote: ... NC-membrane (pore size 0.45 mm) was used for protein transfer using Bio-Rad Mini PROTEAN blotting system (Bio-Rad). Proteins were transferred for 1 h at 300 mA in transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified and the same amount of protein was loaded to the wells in a 4-15% SDS-PAGE gel (BioRad). Protein was transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... depending on the proteins analysed) and transferred on 0.2 μm nitrocellulose membranes (Trans-Blot® TurboTM Transfert Pack, Bio-Rad). Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau ...
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bioRxiv - Cell Biology 2020Quote: Purified ORB proteins were concentrated in a stacking gel using SDS-PAGE stained with Coomassie Brilliant Blue R-250 (Biorad). Proteins in the excised gel band were subjected to in-gel digestion as follows ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Immunodetected proteins were identified using the enhanced chemiluminescence system (Clarity Western Blotting ECL Substrate, Bio-Rad Laboratories Ltd, Watford, UK) or Odyssey imaging system (LI-COR) ...
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bioRxiv - Cell Biology 2022Quote: ... as well as respective inputs (20 µl of extracts corresponding to 50 µg of total proteins) were separated using SDS PAGE electrophoresis and transferred onto nitrocellulose membrane by electro-blotting (SemiDry, BioRad). Western blots were probed with rabbit anti-GFP (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...