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Citations for Bio-Rad :
8201 - 8250 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed directly in sample buffer and protein extracts were separated on a 4-20% polyacrylamide gel (Bio-Rad). Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53 ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were detected by scanning the gel for green (GFP) or red (mCherry) fluorescence using a ChemiDoc MP system (BioRad) and proteolytic cleavage was assessed from the changes in banding patterns ...
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bioRxiv - Genetics 2020Quote: ... TGX stain-free gels had total protein visualized prior to transfer and analyzed on Image Lab software (Image Lab 6.0, Bio-Rad) 41 ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: Tissue lysates (30 μg protein) were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against ROCK1 (Cat# ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 to 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 was use to block the membranes ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total protein/sample were loaded in each well of a 10% PAGE gel and transferred onto a nitrocellulose membrane (#1704158, BioRad) in a Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... ECM and RPE cell lysate (15-20 μg protein) were run separately on precast Mini-Protean TGX gels (Bio-Rad) and transferred onto PVDF membrane (Immobilon) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were separated in 10% SDS-polyacrylamide gels and electrotransferred to PVDF membranes using a semi-dry system (Bio-Rad). Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a nitrocellulose membrane using a Mini Trans-Blot Cell Wet/Tank blotting system (BioRad). After transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
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bioRxiv - Biochemistry 2021Quote: ... Ni-NTA and FLAG elutions were analyzed by SDS-PAGE on AnykD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). The samples were not heated before loading to preserve the fluorescence of the GFP and mCherry tags ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized via chemiluminescence (ECL Enhanced Chemiluminescence; Pierce ECL Western Blotting Substrate) using the ChemiDoc MP system (BioRad).
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bioRxiv - Developmental Biology 2021Quote: ... Loading was normalized to total protein for monoubiquityl-Histone H2A and Ubiquitin blots using AnyKD TGC Stain-Free gels (BioRad). Loading for monoubiquityl-Histone H2B blots was normalized to mouse anti-β-tubulin (1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... The amount of ATP for one cell was derived from the amount of protein in one cell found via Bradford assay (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were separated on 10 or 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... and equal volume and quantity of protein samples were made by addition of 4x Laemmli Sample Buffer (#1610747, Bio-Rad) containing 2-merchatoethanol (#1610710 ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Supersignal West Femto (Pierce) incubation was used to visualize the protein bands of interest on the ChemiDoc XRS+ Imaging system (Biorad). Images acquisition and densitometry analysis was performed using the Image Lab software (Biorad).
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bioRxiv - Cancer Biology 2020Quote: ... Around 40 μg of whole-cell lysate was resolved in 10% SDS-PAGE gel (Mini protein Tetra cell Bio-Rad) and blotted to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... and the separated proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The immunodetected protein bands were then analyzed using ChemiDoc XRS system with Quantity One software (Bio-Rad, Richmond, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... and 30 μg of total protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). Correct protein loading was confirmed by Ponceau staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled with SDS sample buffer and resolved on a 10% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
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bioRxiv - Microbiology 2021Quote: hCMP-mRBD protein kinetic binding studies to ACE2-hFc and CR3022 antibody were performed on a ProteOn XPR36 Protein Interaction Array V.3.1 (Bio-Rad). The GLM sensor chip was activated with sulfo-NHS and EDC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and vasopressin) peptides were spotted onto a 0.2-μm polyvinylidene fluoride membrane (Immun-Blot PVDF Membrane for Protein Blotting, BIO-RAD). The membrane was air dried at room temperature and was washed for 10 min in 0.05 M Tris buffer (pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... gels were subjected to a western blot in which protein was transferred onto a 0.45 μm nitrocellulose membrane using a Mini Trans-Blot Cell (Bio-Rad), in transfer buffer in the presence of an ice block for 1 hour at a constant current of 255 mA ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were affinity purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared in Licor protein sample loading buffer and run on TGX 7.5% and 4-20% Tris-glycine gels (Bio-Rad) and blotted onto a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... 10 µg of protein were loaded in each well of a gel (Bio-Rad # 567-1094, 567-1095, 456-1036). Blots were blocked in 5% milk/TBST for two hours at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RIPA buffer and protein extracts from equal numbers of cells were resolved on a 12% SDS-PAGE gel (Biorad), followed by transfer onto Immun-Blot PVDF membranes (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... after which proteins were transferred to polyvinylidene fluoride (PVDF) membranes (catalog no. 162-0177; Bio-Rad Laboratories; Hercules, CA, USA). Membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... the amount of GFP-bound protein was assessed by loading final extract in sample buffer (without boiling) on a Mini-PROTEAN TGX Precast Gel (BioRad), visualised with LED488 illumination (G-box ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated on a 4–20% SDS-PAGE gel (mini-PROTEAN TGX gels, Bio-Rad) along with PageRuler Prestained protein ladder (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed with 4-20% Mini-PROTEAN(R) TGX(tm) Precast Protein Gels (Bio-rad, #4561095 or #4561096) and Trans-Blot ® Turbo™ Mini PVDF Transfer Packs (Bio-rad #1704156) ...
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bioRxiv - Bioengineering 2022Quote: ... and the nanocluster size was derived by eluting 20.0 μL of the sample with PBS buffer at a flow rate of 0.5 mL/min and calibrating the chromatograph with that of the protein standards (Bio-Rad). The hydrodynamic size and zeta potential of supraclusters were determined using a Zetasizer NANO ZS-90 instrument (Malvern) ...