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Citations for Bio-Rad :
8301 - 8350 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and purity was determined using SDS-PAGE on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) stained with Biosafe Coomassie (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated in SDS-PAGE gels were transferred onto a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The protein concentrations in each cell extract were measured with the Bradford assay (SmartSpec Plus spectrophotometer, Bio-Rad, Hercules, CA) using the Bradford dye reagent and bovine standards (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... The adapted protein quantity per lane was loaded into 4-20% 1.5 mm Tris-glycine 15 well mini gels (Bio-Rad) with 1X Laemmli sample buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 μg protein per sample were loaded onto SDS-page gels for protein separation and then transferred onto nitrocellulose or methanol-activated PVDF membranes via full-wet transfer assay (BioRad) or semi-wet transfer ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 180 V for one hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 30µg of total proteins were loaded onto each well of Mini-PROTEAN TGX Stain-Free Precast Gels (10%, Biorad, # 4568031), and migrated for 45 min at 120 Volts ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by electrophoresis through 12% agarose polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad Cat #1620175) by electroblotting ...
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bioRxiv - Immunology 2024Quote: ... protein was transferred to a 0.45-μM nitrocellulose membrane (Cytiva) with a Transblot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked with 5% BSA and 0.1% Tween 20 (IB blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Developmental Biology 2023Quote: ... and sample aliquots were diluted 1:50 in water before Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were normalized to 1 mg/mL in 0.1% Azo ...
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Genetics 2023Quote: ... The supernatant was transferred to a fresh 1.5 ml Eppendorf tube and protein concentration was measured using Bradford assay (Bio-Rad Protein assay Dye reagent Concentrate (#5000006 ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were visualized by Coomassie blue staining and ATG3 monomer and dimer were quantified using the ImageLab software (Bio-Rad) and the sample treated with 20 mM DTTred and 0 mM DTTox (Eh= -∞ ...
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bioRxiv - Plant Biology 2023Quote: ... or the proteins were transferred onto a nitrocellulose membrane using Mini Trans-Blot® cell (Bio-Rad, Hercules, CA, USA). The transfer was performed in 1x transfer buffer at 250 mA for 1 h at 4 °C under constant stirring ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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bioRxiv - Plant Biology 2023Quote: ... proteins separated in SDS-PAGE gels were transferred to a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). For anti-His and anti-GFP blots ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were again washed three times with TTBS buffer and protein bands were visualized using the ChemiDoc Imaging System (BioRad) and quantified with Image Lab software (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane using a Trans-blot Turbo system (Bio-Rad, 1704159). All Western blots were revealed using ECL (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4–12% gradient bis-tris gels (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 5uL of protein sample was loaded onto a 4-15% Mini-PROTEAN TGX Precast polyacrylamide gel from Bio-Rad (4561086). Protein samples were resolved and transferred to a PVDF membrane using the Trans-Blot STurbo Transfer System ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were spun down and the supernatant was used to measure protein concentration by using a Bradford Assay (BioRad). Equal protein concentrations were loaded onto a NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were stained with Ponceau S and total proteins were visualized using the ChemiDoc™ MP imaging system (Bio-Rad). To detect proteins of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... Separated proteins were transferred into a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were combined with Laemmli buffer and separated on a 4-15% Mini-PROTEAN TGC precast gel (Bio-Rad) by electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of crude cell extracts was measured using the Bradford method with BSA as a standard (Bio-Rad). The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem ...
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bioRxiv - Cell Biology 2023Quote: ... Then the proteins were transferred to a PVDF membrane using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Pack (BioRad, 1704156). The membranes were blocked in 5% non-fat milk in TBST for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were analyzed by SDS-PAGE and purities determined by densitometry of Coomassie Blue-stained gels (Image Lab Software, BioRad). Protein batches used in this work were ≥ 90% pure.
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Samples containing 20 µg of protein were separated on 4-20% Tris-Glycine SDS-PAGE gel (Bio-Rad, CA, USA) and transferred to PVDF membrane using Trans-Blot Turbo transfer system and transfer packs (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD). Blots were probed overnight at 4°C with antibodies recognizing GAD67 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 15 μg of total protein per sample was loaded into separate lanes on a precast 10–20% electrophoresis gel (Tris-HCl polyacrylamide, BioRad). The blotRig software helps counterbalance sample positions across the gel by treatment condition. ...
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bioRxiv - Plant Biology 2023Quote: ... The temperature ramp rate was 1°C/20 s and protein unfolding was monitored by the increase in RFUs produced every 10 s in a CFX Opus 96 System (BioRad). The average trace of 6 replications (2 at each concentration ...
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bioRxiv - Genetics 2023Quote: ... 30 µg of protein were diluted in Laemmli buffer and loaded in a 4 % to 15 % gradient mini-protean TGX gel (BioRad). After electrophoresis run ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to PDVF membranes using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs (BioRad, Cat. No. 1704156) and the BioRad Transblot system ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was transferred to a new Eppendorf tube and protein was quantified with Quick Start Bradford 1X Dye Reagent (BioRad). Samples were added to 4X Laemmli Sample Buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2023Quote: ... the total protein concentration was determined by Bradford assay using the Quick Start Bradford 1x Dye reagent (#5000205, Bio-Rad) and BSA (#23209 ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 50 to 100 ug protein was loaded in each lane and separated on 4–20% precast polyacrylamide gel (17000436, Biorad) and transferred on PVDF membrane (1704156 ...