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Citations for Bio-Rad :
8001 - 8050 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Samples were run on 7.5% Mini-PROTEAN gels for cytoplasmic proteins and 12.5% Mini-PROTEAN gels for histones (Bio-Rad, #4568025). Primary antibodies used ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were desalted into phosphate-buffered saline (PBS) pH 7.4 using Econo-Pac 10DG gravity flow chromatography columns (Bio-Rad). Absorbance ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were denatured and proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on Mini-Protean 10% tris-glycine gels (BioRad). Precision plus protein Kaleidoscope molecular weight (MW ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of total protein lysate was loaded on to an TGX Stain Free FastCast Acrylamide gel (BioRad, cat# 1610185) and transferred using an iBlot2 gel transfer device (Thermofisher scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Lyophilized proteins were dissolved in SDS-PAGE sample buffer and resolved by SDS-PAGE (BioRad precast 4-15 % gradient gel). The gel was stained with Simple Blue Safe Stain (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Genetics 2021Quote: ... Proteins (7 μl of extract) were separated on Laemmli SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, 1620112). Membranes were stained with Ponceau-S for visualization ...
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bioRxiv - Genetics 2021Quote: ... and 10 µg protein were resolved by SDS-polyacrylamide gel electrophoresis and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad). The membrane was blocked in 5% skimmed milk powder in PBX (0.01% Triton X-100 in PBS ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 OD equivalents of the resulting sample was subjected to SDS-PAGE and the proteins were separated on 4-15% Mini-PROTEAN-TGX strain-free gels (BioRad). For subsequent immuno-blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Denatured protein samples were then resolved in a 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, #1620177). Blots were probed by custom-designed Drosophila antibodies as previously described 6 diluted in PBS-0.1% Tween supplemented with 5% nonfat milk ...
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bioRxiv - Pathology 2022Quote: ... Appropriate total proteins from different lines were resolved on 10 % polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad). Anti-FLAG monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and equal amount of proteins were separated by SDS/PAGE on pre-cast 4-20% polyacrylamide gradient gels (Cat# 5671094, Biorad) and transferred to immune-blot PVDF membrane (Merck cat# IPVH00010) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by gel electrophoresis on a 4 to 20% polyacrylamide gradient Mini-Protean TGX precast gel (Bio-Rad) and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivities of the blotted proteins were detected with Clarity Western ECL Substrate for Chemiluminescent Western Blot Detection (Bio-rad, U.S.A.) and ChemiDoc XRS+ system (Bio-rad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
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bioRxiv - Molecular Biology 2021Quote: ... the obtained protein bands were transferred onto a 0.22 μm nitrocellulose membrane (MDI) using a Trans-Blot Turbo Transfer System (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Cell Biology 2019Quote: ... Polyacrylamide gels used in this study were either prepared using 10% EZ-Run protein gel solution (Fisher) or precast gradient gels (4-20%, from Biorad). Any quantification performed on western blots was done using ImageJ software ...
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bioRxiv - Biochemistry 2019Quote: ... Protein content was measured using the BCA kit and 50 micrograms of protein per sample were loaded on a Bio Dot apparatus (Biorad) equipped with a 0.2 micron pore size nitrocellulose membrane soaked in buffer B (10 mM Tris-HCL pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentrations of the DMSO control samples were measured in triplicate using a detergent compatible (DC) assay (Bio-Rad, USA). Then ...
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bioRxiv - Cell Biology 2019Quote: ... and 25-30 μg of protein were loaded on a 7.5% separating gel using Mini Trans-Blot Cell (Bio-Rad). Nitrocellulose membranes were stained with primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... The target proteins were detected with the Clarity™ Western ECL Blotting Substrate (#170-5061, Bio-Rad, Hercules, CA, USA). The sizes of proteins were estimated with a PageRuler™ Prestained Protein Ladder (10-180 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples (10 µL) were run on 12.5% SDS-PAGE gels along with a Precision Plus Protein dual colour standard (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ Fast-Cast™ gels (Bio-Rad). Separated proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2019Quote: ... The exchanged sample was diluted to either 0.8 or 0.4mg/ml via the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, CA). Samples were divided into 100µL aliquots and flash frozen with liquid nitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell extracts were prepared using UREA lysis buffer and 30-60μg of total protein was loaded on a 7.5% or 12% denaturing polyacrylamide self-cast gel (Bio-Rad, Switzerland). Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074 ...
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bioRxiv - Molecular Biology 2019Quote: ... protein samples were separated by SDS-PAGE and electrophoretically transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA). After 1-h blocking ...
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bioRxiv - Physiology 2020Quote: ... Equal amounts of protein were resolved on SDS-PAGE (10% Mini-PROTEAN® TGX Stain-Free™ Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... The spin-labeled protein was further concentrated and free label was removed using Micro BioSpin 6 Chromatography Columns (Bio-rad) pre-equilibrated with buffer C.
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bioRxiv - Systems Biology 2019Quote: ... Equal amounts of protein were loaded onto the acrylamide gel (Bio-Rad Mini or Midi PROTEAN® TGX precast gels). For the transfer of proteins to the PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: Isolated dermal endothelial cells were lysed in Laemmli sample buffer and proteins were separated on a 7.5% Mini-Protean TGX precast gel (#456-1025, Bio-rad) using Tris/glycine/SDS running buffer (#161-0732 ...
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bioRxiv - Immunology 2019Quote: ... and 500 ng protein was loaded to 10% TGX (Tris-Glycine eXtended) Stain-Free FastCast polyacrylamide gels (Bio-rad, # 1610183), 200 V running for 45min ...
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bioRxiv - Bioengineering 2020Quote: ... A pre-stained molecular weight marker was run in parallel to determine the molecular weight of the proteins (BioRad, #1610375).
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and western blot analysis was performed using the ChemiDoc XRS+ imaging system (BioRad). Western blots were analyzed using Image Lab version 5.2.1 software (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... equal amounts of protein were subjected to electrophoresis on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Membranes were treated with antibodies using standard Western blot techniques ...
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bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... The gel-separated protein bands were transferred on to a 0.2 μm nitrocellulose (Bio-Rad; Cat# 162-0112, Hercules, CA) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...