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Citations for Bio-Rad :
7951 - 8000 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from the dissected testes using RIPA buffer as described previously and quantified using Bradford reagent (Biorad). Equivalent concentration of lysate was denatured using 1X Laemelli Buffer with 1 M DTT at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were treated with appropriate antibodies for detection and analyzed using a ChemiDoc XRS+ image analyzer (Bio-Rad). Band intensity was measured using Quantity One software (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... SDS-PAGE was followed by transfer of proteins on a nitrocellulose membrane with a semi-dry blotting system (BioRad) and protein expression was analyzed by incubation with the following primary antibodies ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng of each purified protein was mixed with an appropriate volume of 2x Laemmli sample buffer (Bio-Rad) with 5 mM β-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of each sample was loaded onto a 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 180 V for 45 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples prepared with equal amounts of proteins were run on 10% Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033) (for ASGR1 blot ...
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bioRxiv - Microbiology 2024Quote: ... and total protein was wet transferred to a nitrocellulose membrane using a mini Trans-Blot electrophoretic system (Bio-Rad). After the transfer ...
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bioRxiv - Immunology 2024Quote: ... The SEC-eluted protein peaks corresponding to the correct retention volume based on the molecular weight standards (BioRad: 1511901) were collected and concentrated using Amicon Ultra centrifugal filters (Millipore Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and at 100-120 V through the resolving gel until the protein xtra prestained ladder (Bio-Rad catalog # 1610377) achieved good separation ...
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bioRxiv - Microbiology 2024Quote: Protein fractions were analyzed by standard denaturing SDS-PAGE electrophoresis using 4 to 20% gradient mini-gels (Bio-Rad), InstantBlue Coomassie protein stain ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to PVDF membranes by wet electroblotting (Bio-Rad). To improve signal detection ...
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bioRxiv - Biophysics 2024Quote: ... Equal volumes of protein samples were resolved on 4-12% gradient SDS-polyacrylamide gel and transferred onto 0.2μM nitrocellulose (BioRad #1620112) or PVDF (BioRad #1620177 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad) using Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on a 4 to 20% Trist-glycine stain free polyacrylamide 10-well gel (Bio-Rad) at 170 V for approximately 1 h in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084), and the gel was stained using QC Colloidal Coomassie Stain (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (Bio-Rad). Purified protein was stored in Gel Filtration Buffer + 25% (v/v ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... were used to reveal proteins transferred on nitrocellulose membrane with the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150). Membranes were blocked in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Microbiology 2024Quote: ... The protein lysates were centrifuged and the supernatants were subjected to SDS-PAGE (gradient, 4 to 20%; Bio-Rad) and electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded to a 48-well Bio-Dot® microfiltration apparatus (Bio-Rad Laboratories, Hercules, CA, USA) with BioTraceTM NT nitrocellulose membrane (Pall Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome pellet was resuspended in same lysis buffer and protein concentration was determined via Bradford Assay (Bio-Rad, #5000205) prior to western blot.
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bioRxiv - Microbiology 2024Quote: Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) and then blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were electrotransferred using a Trans-Blot® Turbo™ Mini PVDF Transfer Pack and System (Biorad). After blocking for 1 h in 0.5% Tween-PBS in 5% skim milk (blocking solution) ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred to PVDF membranes using Trans-Blot Turbo PVDF Transfer Packs (Bio-Rad, Hercules, CA, USA), and the membranes were blocked with Blocking One (Nacalai Tesque) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a polyvinyldiene fluoride (PVDF) membrane in a Mini Trans-Blot cell (Bio-Rad, CA, USA) at a constant current of 315 mA on ice for 90 min ...
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bioRxiv - Cell Biology 2024Quote: ... 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad) to a nitrocellulose membrane (0.45 µm ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Plant Biology 2024Quote: ... Protein was transferred to 0.45 µm nitrocellulose membrane (Cytiva Amersham Protran #10600003) using a tank system (Biorad #1703930, #1704070) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: hCOs were processed using urea-based lysis buffer and proteins were separated in the SDS-PAGE gels (Bio-Rad) and nitrocellulose membranes.
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bioRxiv - Physiology 2024Quote: ... and 30 μg protein were loaded per well in Mini-PROTEAN TGX Stain Free precast gels (Bio-Rad, 4868096). Proteins were transferred to nitrocellulose membranes using Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to PVDF membranes with NuPAGE Transfer Buffer in a Trans-Blot Turbo transfer apparatus (Bio-Rad) as per the manufacturer’s instructions ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The same amount of protein was loaded in each lane of Mini-PROTEAN TGX gels (4–20%, Bio-Rad). The antibody signals were detected by chemiluminescence using ImmunoStar LD reagent (290-69904 ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated by SDS–PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein lysates were then loaded onto an 10% Ready Gel® Tris- HCl Precast Gels (BioRad, catalog#1611119) in running Buffer (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate were separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BIO-RAD), transferred to polyvinylidene difluoride membranes (0.2 um ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Biophysics 2021Quote: ... protein was further purified by a Hydroxyapatite column (HA) connected to an NGC Medium-Pressure Liquid Chromatography System (BIO-RAD). A gradient form 5 mM NaPi ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). The nitrocellulose membranes were blocked in 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 ...
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bioRxiv - Biophysics 2020Quote: ... proteins from the supernatant were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and chemiluminescent signal was acquired with a Chemidoc Imaging System (Bio-Rad). Images were processed with the ImageLab software (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4-12% gradient bis-tris gels or 16% tricine gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Heat-induced protein denaturation curves were obtained using a CFX Connect Real-Time PCR system (Bio-Rad Laboratories, Hercules, CA) as described previously 99 ...