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Citations for Bio-Rad :
7851 - 7900 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Two thirds of eluted protein sample from HA Immunoprecipitation were run on a 4-15% Mini-PROTEAN (Bio-Rad) gel to separate proteins and remove synthetic HA peptide ...
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bioRxiv - Physiology 2022Quote: ... proteins were separated by running the gel in an electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad) at 200 V for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... The pelleted protein G Sepharose beads were then resuspended in 50 µl of 2x Laemmli sample buffer (Bio-Rad) and proceeded to SDS-PAGE and immunoblotting analysis.
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Protein band intensities were quantified using ChemiDoc MP Imaging System and Image Lab software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The gel in Figure 7B was run on a 12% Mini-Protean TGX precast protein gel (Bio-Rad, #4561034), whereas the one in Figure 7C was run using hand-cast gels ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amount of protein samples was separated on SDS-PAGE gel (12%) electrophoresis and transferred to nitrocellulose membrane (BioRad). Blots were blocked with 5% skim milk for 1 hour at room temperature and probed with antibodies at 4℃ overnight against the desired proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing pure protein were determined via SDS-PAGE analysis on a 4-20% gradient gel (Bio-Rad), pooled ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein samples were fractionated by electrophoresis through agarose gels and transferred to PVDF membranes (Bio-Rad). After blocking with 5% dry milk for 2h ...
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bioRxiv - Microbiology 2024Quote: ... SDS-Page total protein content was imaged using standard Coomassie brilliant blue staining with Kaleidoscope molecular weight ladder (Biorad). Antibodies tested to visualize M ...
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bioRxiv - Neuroscience 2024Quote: ... 7.5 μg protein was loaded onto precast 15-well 4-15% Mini-Protean TGX Stain Free Gels (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... 6 μg of protein from each sample was loaded into a 4-20% Mini-PROTEAN® TGX gel (BioRad) and proteins were separated by size using electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were separated by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-PROTEAN kit (1658001FC, Bio-Rad). Between 20 and 50 μg of protein per well were loaded ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... for high-sensitivity chemiluminescent protein detection with Uvitec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
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bioRxiv - Molecular Biology 2024Quote: Input lysates (5 μg of total protein) and entire elution products were combined with 4x Laemmli buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... and the samples were separated on 4-20% or 4-15% Mini-PROTEAN TGX Precast Protein Gels (BioRad Laboratories). The gel was transferred to a PVDF membrane using the iBlot transfer system (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized using ECL-based autoradiography film system (Super RX-N, Fujifilm) or ChemiDocTM Imaging system (BioRad) and analyzed using Gel Analyzer plugin from ImageJ (Fiji) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were prepared for SDS-PAGE separation by denaturation with 2x Laemmli sample buffer (Bio-Rad Laboratories; 161-0737) with 355mM β-mercaptoethanol for 5min at 95°C.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were separated on Mini-PROTEAN® TGX™ Precast Gels (4–20% gradient, BioRad Cat. No. 4561091) in Tris-SDS-glycine buffer ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... and an equal amount of protein was resolved in 4-12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (GE Amersham) by semi-dry transfer for 10 min at 15V (Bio-Rad), air dried ...
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bioRxiv - Microbiology 2023Quote: Proteins from LEVs and SEVs were separated on a precast 4-15 % SDS-PAGE (Bio-Rad, Hercules, CA USA) and stained with Coomassie G-250 ...
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bioRxiv - Molecular Biology 2024Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were measured using Pierce BCA Protein Assay Kit and equal amount of protein (10-20μg) mixed with Laemmli sample buffer were loaded in 4-20% TGX precast gels (Biorad) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on an SDS-PAGE gel and transferred to a nitrocellulose membrane (BIO-RAD, Hercules, CA, USA). The membrane was blocked with EveryBlot blocking buffer (BIO-RAD ...
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bioRxiv - Cell Biology 2023Quote: ... BioRad Clarity Western ECL substrate was added to membranes to visualize protein using Bio-Rad VersaDoc Imaging System (BioRad) or iBright Imaging System (Thermofisher).
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... SDS-PAGE was followed by transfer of proteins on a nitrocellulose membrane with a semi-dry blotting system (BioRad) and protein expression was analyzed by incubation with the following primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were treated with appropriate antibodies for detection and analyzed using a ChemiDoc XRS+ image analyzer (Bio-Rad). Band intensity was measured using Quantity One software (Bio-Rad) ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and equal amounts of protein (13 µg per lane) separated on gradient polyacrylamide gels (Bio-Rad, Hercules, CA, USA). Samples were then transferred to Immobilon-FL transfer membrane (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... The samples (22.5 µg – 85 µg) were loaded on 4-20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad), ran for the first 30 minutes at 70 V and the next 4 hours at 100 V in Tris-Glycine SDS buffer ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resolved on a 4% to 20% Mini-Protean TGX precast protein SDS-PAGE gel (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: Denatured parasite proteins were resolved on 10% SDS-polyacrylamide gel and transferred to 0.2 μm nitrocellulose membranes (Bio-Rad) using wet-transfer in Towbin buffer (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad™) alongside size standards (Precision Protein Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were blotted on a polyvinylidine fluoride (PVDF) membrane with a Trans-Blot Turbo Transfer System (Bio-Rad). The protein coated membrane was blocked with 5% normal serum (Sigma ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Soluble proteins contained in the supernatant were separated and mixed with 4x Laemmli sample buffer (Bio-Rad, 161-0747) before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Physiology 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Proteins were transferred from the gel to an ethanol-immersed PVDF membrane in transfer buffer (30 min ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples (10 μL each) were run on 4-20 % Mini-PROTEAN TGX Stain-FreeTM precast Gels (BioRad) in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes via semi-dry transfer (Bio-Rad Trans Blot Turbo; 25 V, 30 min) for western immunoblot analysis ...
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bioRxiv - Neuroscience 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... and Tris-Glycine SDS buffer (Geneflow B9-0032) or 4–12% Criterion™ XT Bis-Tris Protein Gels (BioRad) and XT MES running buffer (BioRad 1610789) ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 13.2K rpm and protein concentration was determined by BCA assay (Bio-Rad, Hercules, California, USA). For western blot analysis ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then electro-transferred onto PVDF (polyvinylidene fluoride) membranes with Trans-Blot Turbo transfer system (Bio-Rad Laboratories). Antibodies used for the detection are listed in Table S1 ...
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bioRxiv - Genetics 2023Quote: ... and the protein bands were detected using the chemiluminescent Clarity Max™ Western ECL Substrate (Bio-Rad, Cat# 1705062S). The bands were visualized in the ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: Protein extractions were freshly prepared under denaturing conditions by first diluting samples in 4x Laemmli Sample Buffer (Biorad #1610747) containing 50mM β-mercaptoethanol (BME ...