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Citations for Bio-Rad :
7701 - 7750 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of total protein underwent separation by 10% SDS-PAGE and electro-transfer on nitrocellulose membranes (Bio-Rad). After blocking (5% skim milk in Tris-buffered saline with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... 10-20 µg of total protein was loaded on precast SDS-PAGE gels (4-20% Criterion TGX, Bio-rad) and immobilon-FL membranes (Merck Milipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ precast gel (Biorad #456-1094,), transferred onto methanol pretreated PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were cleared by centrifugation at 20,000 x g for 30 min and protein amount was determined by standard Bradford assay (Biorad). For each reaction ...
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bioRxiv - Cell Biology 2021Quote: ... was added to membranes to visualize proteins by chemiluminescence using X-ray film or a Chemidoc station (Bio-Rad). Each immunoblot was repeated several times using samples from independent experiments ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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bioRxiv - Physiology 2020Quote: ... and isolated protein complexes were eluted from the beads in 1X SDS sample buffer (Bio-Rad Laboratories, Hercules, CA) at 60°C for 10 min ...
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Functional characterization of a DNA-dependent AAA ATPase in SimranZ1, a F cluster mycobacteriophagebioRxiv - Biochemistry 2021Quote: ... The protein was then transferred to a PVDF membrane by using Mini-PROTEAN Tetra Cell (Bio-Rad, United States). The membrane was blocked overnight by using 5% BSA at 4 °C followed by incubation with mouse anti-His6 antiserum (Santacruz Biotech ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins were purified using a Profinia immobilized metal affinity chromatography (IMAC) column with Ni2+ beads (Bio-Rad) and a Profinia protein purification system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using 12% polyacrylamide gels and transferred onto a PVDF membrane using Trans-Blot Turbo (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then electroblotted onto a PVDF membrane using a semidry blotter (trans-blot turbo transfer system; Bio-Rad). Membranes were blocked overnight at 4°C in TBS-T (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... and 30 μg of total protein was separated by 12 % SDS-PAGE and blotted onto PVDF membrane (Bio-Rad). The membranes were blocked in 5 % (w/v ...
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bioRxiv - Systems Biology 2020Quote: ... As a molecular weight marker we utilized the Precision Plus Protein™ Prestained Standard in Dual Color (Biorad, 1610374).
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated samples were separated by SDS-PAGE using Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, 4569034), and then blotted to a PVDF membrane (0.2 μm ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of each homogenate was measured using a Bradford assay according to the manufacturer’s instructions (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred at room temperature (RT) onto a nitrocellulose membrane using a semi-dry transfer cell (Biorad) (20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein bands were then transferred from the gels onto 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, CA, USA) using semi-dry electroblotting system (Trans-Blot Turbo Transfer System ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Microbiology 2020Quote: ... Equal quantities of protein (generally 20-50 μg) were resolved on 4-20% gradient SDS-Polyacrylamide gels (Bio-Rad) and then transferred onto Polyvinylidene difluoride membranes (Millipore Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the epithelial cell proteins in the resulting supernatant was determined using the Bradford method (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Twenty μg of each protein sample was separated using SDS-gel electrophoresis and transferred onto a 0.45-μm PVDF membrane (BioRad). Membranes were incubated with blocking buffer (5% milk powder dissolved in 1x TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed at 150V for one hour through 7.5% or 4-20% mini-PROTEAN TGX gels (Bio-Rad) and blotted at 300 mA for one hour onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in SDS-PAGE gel (4-20% gradient polyacrylamide gel electrophoresis, Mini-PROTEAN® Precast Gels, Biorad). Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates and lipid raft protein isolates were prepared by sonication in 4x Laemmli sample buffer (Bio-Rad, 1610747) and then loaded into 10% SDS-polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate that contains 10 μg of protein per sample was mixed with 2x Laemmli Sample Buffer (Bio-Rad) supplemented with 2 M 2-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein levels were quantified to normalize between each well using Bradford reagent (Bio-Rad, Marnes-la-Coquette, France).
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bioRxiv - Systems Biology 2022Quote: ... The supernantant was then added to a new Eppendorf and protein concentration was assessed using the Bradford assay (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were visualized on a 4-15% gradient SDS-PAGE TGX Stain-Free gel (Bio-Rad 456-8086) and then stained with Coomassie blue (Bio-Rad 1610786) ...
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Molecular Biology 2022Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electrophoretically transferred onto a polyvinylidene fluoride (PVDF) membrane using Semidry Trans-Blot apparatus (Bio-Rad, Hercules, CA) at 20 V for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... in equal volume and then subjected to electrophoresis using the 7.5% Mini-Protean TGX Precast Protein Gels (Bio-RAD) in a non-denaturing buffer ...
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Microbiology 2022Quote: ... total protein extracts were subjected to SDS page electrophoresis using 4-20% polyacrylamide gradient gels (Bio-Rad, Watford, UK) and transferred to nitrocellulose membranes using a Trans-blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... 10 µL of 12x and 120x diluted protein prep was then loaded into a 15% SDS-PAGE gel (BioRad) with BioRad Precision Plus unstained standard for comparison ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μg of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad 1620177). Membranes were blocked for 1 h in 5% milk in 1X PBST (or 5% BSA in 1X PBST for western blots of immunoprecipitations ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µg of protein was separated by SDS-PAGE using pre-cast TGX 4-15% gradient gels (BioRad). Protein was transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from the remainder of the gel to nitrocellulose membrane using the Trans-Blot Turbo (Bio-Rad). Membranes were incubated in a 5% milk in 1X Tris-buffered saline ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins where eluted from the beads by directly adding 20 µL of Laemli Sample Buffer buffer 2X (Biorad, #1610747). Samples where then processed for SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were quantified on a BioRad Chemidoc Touch Imaging System using Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Data were normalized to GAPDH expression or total protein determined via UV treatment of TGX Stain-Free gels (Biorad) followed by detection on the transfer membrane.
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on a SDS-PAGE Mini-PROTEAN TGX 4-15% gradient gel (Bio-Rad, catalog #456-1086) along a 1:1 mix of Amersham ECL Rainbow Molecular Weight Markers (Amersham # 95040-114 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were denatured and proteins separated in SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad).