Labshake search
Citations for Bio-Rad :
7651 - 7700 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... 1:10 000 dilution for N-terminal Parkin Ab) was incubated one hour at RT and protein visualized using ClarityTM Western ECL Substrate (Bio-Rad).
-
bioRxiv - Cell Biology 2022Quote: ... The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad, Hercules, CA, USA). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... 50 µg of protein per sample were separated on a 4-15% MP TGX precast cassette (Bio-Rad, 4561083) at 100V for 1 hour 25 minutes before being transferred to a PVDF membrane ...
-
bioRxiv - Cell Biology 2022Quote: ... 30 μg of proteins were loaded and resolved by SDS-PAGE electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked with TBS Tween / Milk 5% for 1 hour and incubated at 4°C with primary antibodies overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... Before the protein transfer was carried out 45 min at 25 V using a Semi Dry Blotter (Bio-Rad), the transfer membrane was pre-incubated 5 min in methanol ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein bands were treated by ECL western blotting detection reagents and visualized by ChemiDoc imaging system (Bio-Rad). Densitometry of the band intensity was further determined via Image J software.
-
bioRxiv - Physiology 2020Quote: ... Overall protein concentration (mg/ml) was measured according to the Bradford method (Bradford, 1976) using BioRad stock (BioRad, Finland) diluted with dH2O (1:5 ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amounts of proteins were loaded and separated by electrophoresis in a 4-15% gradient polyacrylamide gel (BioRad #1653320) or NuPAGE 4-12% Bis-Tris gel (Invitrogen #NP0322) ...
-
bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS-PAGE using a Mini-PROTEAN TGX Gel (4-15% or 8-16%, Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto PVDF membranes for 30 min using BioRad Trans-Blot Turbo system (Bio-Rad) at 110V ...
-
bioRxiv - Neuroscience 2021Quote: ... for high-sensitivity chemiluminescent protein detection with UVItec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
-
bioRxiv - Molecular Biology 2021Quote: ... Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159, Bio-Rad) and blocked for 1 hour (PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... 80 μg of total protein was separated by SDS-PAGE on a 4-15% Criterion TGX Gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... 30–50 μg of protein was resolved in 4-20% TGX gel (Bio-Rad Laboratories, Inc., Hercules, CA, USA) under reducing conditions and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories) ...
-
bioRxiv - Cell Biology 2022Quote: ... sonicated and protein content was analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad, cat. No. 1704158). StrepII-tagged proteins (RBBP6 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The same amounts of protein extracts were separated on the 4%-20% Mini-Protean TGX gel system (Bio-Rad) and transferred to PVDF (0.45 μm pore size ...
-
bioRxiv - Physiology 2019Quote: ... 8 µg of total protein was separated by electrophoresis on a precast 4-15% TGX gradient gel (Bio-Rad) and transferred to a 0.45 µm nitrocellulose membrane ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The detection of the proteins on the membrane was performed using the Clarity Western ECL Substrate kit (Bio-rad). The signals were captured using the Alliance Mini (UVITEC Cambridge ...
-
bioRxiv - Molecular Biology 2019Quote: Proteins were separated by SDS–polyacrylamide gel electrophoresis (PAGE) and transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad). The membrane was blocked with 5% milk in PBX (0.05 % Triton X-100 in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... “any kD” (to quantify GDNF protein) or 7% (to quantify CBP) precast polyacrylamide gel (Bio-rad, Hercules, Cal, USA) and transferred to a polyvinylidene difluoride membrane (PVDF ...
-
bioRxiv - Immunology 2019Quote: ... total proteins were quantified in paw extracts using the Bicinchoninic acid technique (BCA) and 75-100µg of proteins were loaded on 12% polyacrylamide gels (Biorad). The migration step was followed by a liquid transfer on PVDF membranes (100V 40min ...
-
bioRxiv - Neuroscience 2019Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the DC Protein assay Kit (Bio-Rad). 25 µg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... ~5 μg of protein per lane was separated on 4-15% TGX gels (Bio-Rad Laboratories, Hercules, CA, USA) at 200V for 40 minutes in tris-glycine running buffer (Bio-Rad) ...
-
bioRxiv - Biophysics 2019Quote: ... The retention time of both oligomer types was compared with a set of molecular weight protein standards (Bio-Rad) consisting of bovine serum albumin (66.5 kDa) ...
-
bioRxiv - Microbiology 2019Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
-
bioRxiv - Systems Biology 2020Quote: Whole cell fractions were isolated using RIPA buffer and protein concentrations were determined using a Bradford Assay (Bio-Rad). 20µg whole cell lysate were separated on 8-12% SDS-PAGE gels (depending on the molecular weight of the target proteins) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein for immunoblotting was eluted from ten percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from ninety percent of beads using TRIzol reagent (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2019Quote: ... Total proteins (40 µg) were separated using SDS-PAGE (10 % acrylamide) and then blotted onto nitrocellulose membranes (1620112, Biorad). Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001 ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... A total of 10 µg protein/well was separated by electrophoresis on 12% Mini-PROTEAN TGX gels (Bio-Rad). Proteins were transferred to Immobilon-FL PVDF membranes (Millipore ...
-
bioRxiv - Immunology 2019Quote: ... For blotting the SDS-PAGE separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semi-wet transfer (BioRad) and blocked for 1 h with 2% milk ...
-
bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE and protein transfer to nitrocellulose membranes were performed with a Trans-Blot Turbo Transfer System (BioRad, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, 1620115). The membranes were blocked in Tris-buffered saline-Tween 20 containing 5% nonfat milk at room temperature for 1 h ...
-
bioRxiv - Bioengineering 2019Quote: ... and analysed for protein content using 12% SDS-PAGE gels (Mini-PROTEAN TGX Stain-Free Precast Gels, Bio-Rad). Protein gels were imaged using a BioRad Gel Doc EZ Imager and relative protein band intensity was determined using the BioRad ImageLab software.
-
bioRxiv - Genetics 2019Quote: ... Protein samples were run on 15% SDS polyacrylamide gel and transferred to Immun-Blot® PVDF membrane (Bio-Rad) for 1h at 60V ...
-
bioRxiv - Developmental Biology 2019Quote: ... Thirty or 40 µg of protein was resolved on Mini-PROTEAN TGX precast 12% SDS-PAGE gels (Bio-Rad) and proteins were transferred to a nitrocellulose membrane using the Bio-Rad Trans-Blot Turbo Transfer System ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins from peak fractions were pooled and DTT was removed with an Econo-Pac 10DG desalting column (Bio-rad) which was pre-equilibrated with buffer C ...
-
bioRxiv - Biochemistry 2020Quote: Proteins separated by SDS-PAGE were transferred to PVDF membranes using the TRANS-BLOT semidry system from BIO-RAD in transfer buffer (25 mM Tris ...
-
bioRxiv - Cancer Biology 2020Quote: ... 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto PVDF membranes (Millipore ...
-
Sex Differences in Brain Tumor Glutamine Metabolism Reveal Sex-Specific Vulnerabilities to TreatmentbioRxiv - Cancer Biology 2021Quote: ... Membranes were washed with PBS-T again and proteins were visualized using a ChemiDoc MP Imaging System (Bio-Rad). Band intensity was quantified using Image Lab Software (Bio-Rad).
-
bioRxiv - Cell Biology 2020Quote: ... Macronutrient measurements were performed in 96-well plates using commercial kits: DC Protein Assay kit (Bio-Rad, 500-0116), Triglyceride Assay kit (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... Equal amounts of protein samples were separated by 9% SDS-PAGE gel and transferred onto nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 55° C for 5 minutes and 30 µg of protein per sample was run on Tris-Tricine 16.5% precast polyacrylamide gels (BioRad) for Aβ or 10% Tris-Glycine (TGX ...
-
bioRxiv - Cell Biology 2021Quote: ... Twenty μg of protein were separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (BioRad, Cat# 1620112). Membranes were blocked in 2% bovine serum albumin (BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Equal protein loading was confirmed by Coomassie staining of polyvinylidene difluoride membranes (Coomassie Brilliant Blue R-250, Bio-Rad).
-
bioRxiv - Biophysics 2020Quote: ... An amount of 12 μg of each protein sample was applied on 4-20% gradient precast gel (BioRad, USA). Gels were stained using Coomassie brilliant blue G-250 (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein-containing fractions were concentrated by ultracentrifugation and fractionated by an ENrich SEC650 gel filtration column (Bio-Rad) in binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins in column fractions were acetone precipitated and separated by SDS PAGE on 4-15% gradient gels (Bio-Rad) and blotted onto Immobilon PVDF membranes (Merck) ...
-
bioRxiv - Biochemistry 2020Quote: ... The BRPF1 bromodomain protein was purified on glutathione agarose resin in a 25 mL Econo-Column® (Bio-Rad) by first washing the beads extensively with wash buffer containing 20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...