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Citations for Bio-Rad :
7301 - 7350 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... run at 140 V for 60 min before being transferred onto PVDF membranes (100 V for 100 min) (Bio-Rad). Membranes were blocked in 1x TBST (1x Tris-buffered saline ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... proteins in gels were transferred to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and blocked with 5% milk ...
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bioRxiv - Biochemistry 2022Quote: ... DNA and RNA were sheared through sonication and dotted (3 µg) on the nitrocellulose membrane (BIO-RAD, Cat No: 1620112). The membrane was baked at 80⁰C for 2 h and blocked-in phosphate-buffered saline (PBS-Tween 0.05% ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane from SDS- or native-PAGE gels using the Transblot Turbo semidry transfer apparatus (BioRad) at 25V for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred to low fluorescence PVDF membranes with the Trans-Blot Turbo Transfer System according to manufacturer’s instructions (Bio-Rad). Membranes were blocked for one hour with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... We utilized colorimetric detection to visualize the MBP-Vpu band(s) on the membrane after WB transfer: Primary mouse anti-histidine tag antibody (BioRad) and secondary goat anti-mouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... protein lysates were loaded onto 8% SDS-PAGE gel and transferred to a PVDF membrane using a semi-dry transfer system (Biorad) for 10 min at 2.5 amperes ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were washed a few times in TBST before HRP activity was visualized using Clarity Western ECL Substrate (Bio-Rad) on a ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... 10-30 μL of the supernatants (based on protein abundances) were run on an 8% SDS-PAGE gel and transferred to a nitro-cellulose membrane (Cat. No. 1620112, BioRad) using a semi-dry transfer method (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed again three times with TBST and developed using chemiluminescent substrate (ECL-Clarity, Biorad or ECL-Sirius, Advansta).
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked in 10% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were developed with ECL+ and signals were obtained using ChemiDoc XRS+ and analyzed by Image Lab software (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Physiology 2022Quote: ... the electrophoresed proteins were transferred from SDS-agarose gel to a nitrocellulose membrane (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with a semi-dry western blot system (Pierce Power Blotter ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x with 1X TBST for 15 minutes and developed using Clarity Western ECL Substrate (Bio-Rad, #1705060) and Molecular Imager Gel Doc (Bio Rad) ...
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bioRxiv - Immunology 2022Quote: ... then loaded to SDS-PAGE gel and transferred to PVDF membranes using Trans-Blot® Turbo™ Transfer System (Biorad). Primary antibodies anti-VDAC (D73D12 ...
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bioRxiv - Microbiology 2022Quote: ... The protein gel was then transferred to a 0.45 μm pore-sized nitrocellulose membrane using the Trans-Blot® TurboTM System (Biorad), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... before being resolved by tris-glycine SDS-PAGE and transferred onto nitrocellulose or PVDF membranes at 25V using a semidry Trans-Blot Turbo (BioRad). Membranes were blocked for 1 hour with milk (5% w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein was determined directly on the membrane using the 4-15% Mini-PROTEAN TGX-Stain Free Protein Gel system (BioRad). Antibodies used for Western blotting analysis were ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was washed for 5 min with TBS-T and then imaged using a ChemiDoc XRS+System (Bio-Rad). For chemiluminescence-based detection of immunoblots ...
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bioRxiv - Physiology 2023Quote: ... The membrane was washed three times in PBS with 0.1% Tween-20 and imaged using a ChemiDoc Imaging System (Bio-Rad). Plasma membrane enriched oocyte membrane protein was prepared as described (38).
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bioRxiv - Neuroscience 2022Quote: ... the gels were wet transferred at 400 mA for 90 min to methanol-activated 0.2 µm pore size PVDF membrane (Bio-Rad). Blots were blocked in 5% nonfat milk in Tris-buffered saline (0.5 mM Tris-HCl [pH 7.5] and 1.5 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of samples were separated by SDS-PAGE and then transferred onto PVDF 0.2 μM membrane using Turbo Transblot apparatus (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride membrane (Cat# 1620177, BioRad). Following the protein transfer ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Samples were transferred onto a polyvinylidene difluoride membrane activated with methanol using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were probed with mouse StrepMAB-Classic antibody diluted 1:3000 in TBS-T (25 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated in SDS-PAGE gels were transferred onto a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature and constant voltage (100 V) in 1X MOPS Running buffer and transferred to a nitrocellulose membrane (0.45 mM, Bio-Rad) using iBlot2 system (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... We denatured the lysates and resolve using 10 % (v/v) SDS- PAGE before blotting on PVDF membranes using TransBlot Turbo (BioRad) for transfer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a hybond ECL nitrocellulose membrane using a wet transfer system (Bio-Rad Laboratories, Hercules, CA, USA). The membrane was incubated in α-Ty antibody (1:10 ...
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bioRxiv - Neuroscience 2024Quote: ... electrophoretically resolved within reduced and denaturing conditions and transferred to 0.45 μm pore nitrocellulose membranes by using Mini-Protean system (Bio-Rad), for 90 min at 380 mA ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3x with 10 mL of TBS-T and developed with Clarity Max ECL substrate (Bio-Rad). Western blot images were taken with an Azure Biosystems C400 imager.
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bioRxiv - Molecular Biology 2024Quote: ... Gels were transferred onto nitrocellulose membranes at 25 V for 16 minutes using the Trans-Blot Turbo System (BioRad 1704150). The membranes were placed into 10% milk or 10% BSA in TBS-T for one hour to prevent non-specific binding and washed three consecutive times in TBS-T for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was transferred to a Hybond-N+ Nylon transfer membrane (Cytiva) with a Trans-Blot SD blotting apparatus (BioRad) followed by crosslinking at 0.12 J/cm2 in a CX-2000 UV crosslinker (Analytik Jena) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad). Membranes were blocked with 10% skim milk in TBS-T for 2h with gentle agitation at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto a 0.2-µm PVDF membrane using semi-dry transfer in the Trans-Blot Turbo Transfer System (Bio-Rad) and 1× Trans-Blot transfer buffer (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were transferred after SDS-PAGE to a nitrocellulose membrane using Trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs and electro-transfer system (Bio-Rad). Total protein was stained with 0.1 % (w/v ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg of membrane or soluble protein preparations were separated by SDS-PAGE (12.5 % (w/v) polyacrylamide gels) and transferred to a PVDF membrane using the Trans-Blot Turbo (Biorad, USA) at 25 V and1.0 A for 30 min in 25 mM TRIS ...
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bioRxiv - Biochemistry 2023Quote: Protein samples from differential centrifugation and sucrose gradient fractionation as well as cell lysates from APE1 knockdown experiments were resolved on 10 or 12% polyacrylamide gel and then transferred onto AmershamTM ProtranTM nitrocellulose membrane using a wet transfer system (BIORAD) for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...