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Citations for Bio-Rad :
7401 - 7450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo) followed by transferring onto a 0.2 mm PVDF membrane (Bio-Rad) at 25 V ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... Gels were transferred to a nitrocellulose membrane in transfer buffer supplemented with 20% ethanol with the Trans-Blot Turbo transfer system (BioRad). Membranes were blocked 1 h at room temperature in 5% nonfat dry milk in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Biochemistry 2023Quote: Proteins were separated by SDS-PAGE on 10% gels (20 μg protein/lane) and transferred to PVDF membrane (Bio-Rad) for 20 min at 0.1 A ...
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bioRxiv - Biochemistry 2023Quote: ... were separated on 10 % Tricine gels, and then transferred (0.5 A, 25 V 10 min) onto 22 µm PVDF membranes (Bio-Rad). The membranes were washed 3 times with 1x TBST and then blocked with 5 % bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: Proteins were separated by SDS-PAGE on 10% gels (20 µg protein/lane) and transferred to PVDF membrane (Bio-Rad) for 20 min at 0.1 A ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
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bioRxiv - Cancer Biology 2023Quote: ... SDS-PAGE resolved proteins were transferred onto a nitrocellulose membrane using the Trans-Blot Turbo™ transfer system (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the gel was activated and then transferred for 30 min to a Trans-Blot Turbo Mini 0.2 µm nitrocellulose membrane (1704158, Bio-Rad) on a Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hour and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 minutes ...
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bioRxiv - Genetics 2023Quote: Protein samples were resolved on an appropriate percentage SDS-PAGE gel before being transferred to a PVDF membrane using a semi-dry transfer apparatus (TransBlot SD BioRad) at 25 volts for 30 minutes ...
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bioRxiv - Genetics 2023Quote: Protein samples were resolved on an appropriate percentage SDS-PAGE gel before being transferred to a PVDF membrane using a semi-dry transfer apparatus (TransBlot SD BioRad) at 16 volts for 36 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to nitrocellulose membranes by wet transfer using Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Membranes were blocked in 5% milk/TBS-T and incubated with indicated primary antibodies overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The separated proteins were transferred to a Trans-Blot Turbo 0.2 μm PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were washed in TBS supplemented with 0.05% Tween-20 (TBS-T ...
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bioRxiv - Genetics 2022Quote: ... We washed the membrane 3× 5 minutes in TBS-T and 1× 5 minutes TBS before imaging (Bio-Rad ChemiDoc).
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bioRxiv - Immunology 2023Quote: ... eluted proteins were run on 10% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Trans-Blot Turbo Transfer System, BioRad). Membranes were blocked with 5% milk in Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured in Laemmli buffer and separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were blocked with blocking buffer for 1 h in TBST containing 5% BSA or 5% milk ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was incubated with anti-G50 antiserum (1:3,000 dilution) and horseradish peroxidase–linked anti-rabbit immunoglobulin (Bio-Rad Laboratories Inc. ...
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bioRxiv - Microbiology 2023Quote: ... SDS-PAGE was performed in 1X MES buffer and proteins were transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad, 1620112).
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bioRxiv - Biochemistry 2022Quote: ... The lysates were applied to a cellulose acetate membrane with a pore size of 0.2 mm (Advantec Toyo, Tokyo, Japan) using a Bio-Dot SF blotter (Bio-Rad). The membrane was blocked in PBS-T buffer containing 5% skim milk for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Immunology 2023Quote: ... between 20-30 mg protein was run on 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (1620177, Bio-Rad). After air drying to return to a hydrophobic state ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run through 10% SDS-Polyacrylamide gels and transferred to nitrocellulose membranes using a Trans-Blot Turbo apparatus (BioRad). Membranes were saturated for 1 h with PBST buffer (137 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 5 times with 0.1% Tween-20 5 minutes each then imaged using ChemiDoc Imaging system (BIO-RAD).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were run on 12 % SDS-PAGE gels and transferred to 0.45 µm pore nitrocellulose membranes (Bio-Rad 1620251) using a wet transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on a NuPAGE gel at 120 – 150 V and blotted onto a PVDF membrane using a Trans-blot Turbo Transfer Systen (Biorad) in the mixed molecular weight program.
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated via 12% SDS-PAGE under reducing conditions and transferred to a polyvinylidene difluoride (PVDF) membrane using “Wed blotting system” (BioRad). After incubation with primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... separated by sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% of bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was visualized by incubating the membrane with an HRP-labelled anti-rabbit antibody followed by detection using ECL (Biorad) at the Chemidoc (Biorad).
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Biochemistry 2023Quote: The pulldown elution sample were further resolved on SDS-PAGE and followed by transfer onto a PVDF membrane (BIO-RAD). Then membranes were blocked in 5% milk/TBST for 2 hours at room temperature and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... and transferred onto Trans-Blot Turbo Mini 0.2 µm PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and the appropriate program ...
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bioRxiv - Plant Biology 2023Quote: ... diluted (1 μL in 50 μL milliQ-H2O) and deposited into sample wells onto a nitrocellulose membrane disposed into a 96-well Bio-Dot microfiltration apparatus (BioRad) previously soaked with in TBS (0.02 M Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... wet blotting (at 200 mA per gel for 1 hour) was performed to transfer proteins onto 0.2 µm nitrocellulose membranes (Bio-Rad). Total protein staining was performed according to the manufacturer’s protocol (Revert™ Total Protein Stains kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein transfer onto a PVDF membrane was carried out with the Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs (Bio-Rad) and Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated by dual Tris-glycine SDS-PAGE (lower quarter 15%, upper ¾ 10% acrylamide gel and transferred to a P-type PVDF membrane using the BioRad wet transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... and PsaF was immuno-detected (PVDF membrane / Chemiluminescence) and signals corresponding to reduced (alkylated) and oxidized forms were quantified using ImageLab software (BioRad).
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bioRxiv - Biochemistry 2023Quote: ... The membrane was rinsed three times for 10 minutes in TBS followed by the addition of 1 mL ECL solution (BioRad) to the membrane and visualized on the ImageQuant LAS 500.
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed again with TBS-T 3 times and the membrane was visualized with Vilbert Lourmat imaging system following an incubation with enhanced chemiluminescence (ECL) substrate (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were incubated in 5% BSA in tris-buffered saline (TBS)-Tween 0.1% (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins on the gel were transferred to a polyvinylidene fluoride membrane using a Transblot SD semi-dry transfer system (BioRad) at 25V for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and were then transferred to polyvinylidene difluoride (PVDF) membrane using a semi-dry method with a Trans-Blot Turbo system for 7 minutes at 25V (BioRad). The membranes were probed with the rabbit polyclonal antibody against an amino-terminal region of β1 [21] ...
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bioRxiv - Cancer Biology 2023Quote: ... It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with Tris-buffered saline with Tween20 (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE (30-50 μg total protein per lane) and transferred onto a 0.2 μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% skimmed milk for 30 min at RT and treated with primary antibodies (Supplementary Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were loaded on a polyacrylamide gel and transferred to a polyvinylidene difluoride membrane using the mini trans-blot transfer system (Biorad). To probe for specific antigens ...
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bioRxiv - Cell Biology 2023Quote: ... was followed by electrophoretic transfer to Protran nitrocellulose membrane (Cytiva) using the Mini Trans-blot wet-tank transfer system (BioRad) for 70 min at 250 mA in Transfer Buffer (0.02 M Tris ...