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Citations for Bio-Rad :
7251 - 7300 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The membrane was washes in TBS-T three times and incubated 5 min with Clarity Western ECL Substrate (#1705061, Biorad) in order to detect proteins immunoblotted signals at Alliance LD4 (UVItec Cambridge ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were separated by denaturing electrophoresis in 12% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad) with a Mini-Protean Tetra Vertical Electrophoresis Cell at 100V for 1 h at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... The lysates were fractionated by SDS-PAGE and transferred to nitrocellulose membranes using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad). Blots were developed with an ECL system according to the manufacturer’s protocols (Bio-Rad).
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bioRxiv - Synthetic Biology 2021Quote: ... Then proteins were transferred from the gel to polyvinylidene fluoride (PVDF) membrane for 52 minutes at 15 V in Trans Blot Semi Dry Transfer Cell (BioRad). The membrane was cut horizontally along the 25 kDa marker band to allow for probing with two different antibodies ...
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bioRxiv - Physiology 2021Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies and visualized by enhanced chemiluminescence on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to a nitrocellulose membrane using a TransBlot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (Bio-Rad, Cat # 1704158) on the High MW setting of a TransBlot Turbo machine (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE and then transferred on PVDF membranes using the Trans-Blot Turbo blotting system (Biorad). Pyocins S5 and AP41 were detected using rabbit specific polyclonal affinity-purified ɑ-S5 and ɑ-AP41 antibodies ...
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bioRxiv - Microbiology 2020Quote: ... The other gel was transferred to PVDF membrane using a trans-blot turbo transfer system according to manufacturer’s protocol (Bio-rad). Membranes were blocked with 5% milk in TBS buffer containing 0.5% tween-20 for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins migrated for 60 minutes at 200 V and were then transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories) for 2 hours (70 V ...
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bioRxiv - Pathology 2021Quote: ... Cell lysates were separated by SDS-PAGE and transferred onto a PVDF membrane by a semi-dry transfer (Bio-Rad). After blocking with 5% non-fat dry milk in TBST (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... gels (4.5% acrylamide stacking gel and 10% acrylamide resolving gel) and were then transferred to 0.45 µm nitrocellulose membranes (Bio-Rad) using a semi-dry transfer system (Trans-Blot Turbo ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred onto PVDF membrane by wet western transfer using the Mini Trans-Blot® Cell Assembly (Bio-Rad) in buffer containing 25 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... Proteins were transferred to a 0.2 µm polyvinylidene difluoride (PVDF) transfer membrane either using a Trans-Blot Turbo Mini Transfer Pack (Bio-Rad) in a Trans-Blot Turbo (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad), according to the supplier’s recommendations ...
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bioRxiv - Physiology 2021Quote: Tissue lysates (30 μg protein) were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against ROCK1 (Cat# ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated with peroxidase-conjugated secondary antibodies and visualized by enhanced chemiluminescence using Clarity Western ECL substrate (Bio-Rad). Target protein levels were normalized against total protein (as determined by Amido Black staining).
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bioRxiv - Neuroscience 2021Quote: ... 20 to 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 was use to block the membranes ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total protein/sample were loaded in each well of a 10% PAGE gel and transferred onto a nitrocellulose membrane (#1704158, BioRad) in a Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then washed several times in TBT and the signal was revealed using Clarify ECL substrate (BIO-RAD, 1705060) and imaged on a ChemiDoc XRS+ (BIO-RAD) ...
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bioRxiv - Microbiology 2021Quote: ... in 1X TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad). Blocking was performed in a 5% solution of dry milk and 0.05% Tween –1X PBS (1X PBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... The proteins were fractionated by 8% SDS-PAGE gel and transferred to a polyvinylidene difluoride (PVDF) membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad). The membrane was treated with 5% nonfat milk in PBS-T for 10 min for blocking ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were separated in 10% SDS-polyacrylamide gels and electrotransferred to PVDF membranes using a semi-dry system (Bio-Rad). Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a nitrocellulose membrane using a Mini Trans-Blot Cell Wet/Tank blotting system (BioRad). After transfer ...
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bioRxiv - Biochemistry 2021Quote: Eluted samples were resolved by SDS-PAGE using 4-20% gradient gels and then transferred to the nitrocellulose membrane using the Trans-Blot Turbo transfer system (Biorad). Following the blocking for 1 hour in blocking buffer (5% milk in TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... The second SDS-PAGE gel was transferred to PVDF membrane using a semi-dry transfer apparatus TRANS-BLOT (Bio-Rad) at 25 V ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with the washing solution twice and then reacted with 4-chloro-1-naphthol (Bio-Rad Laboratories) in the HRP color development buffer (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... precast protein gels in sodium dodecyl sulfate (SDS) buffer followed by electrophoretic transfer of proteins to nitrocellulose membrane (BioRad, #2895). After blocking in 5% nonfat dried milk diluted in tris-buffered saline and 1% tween 20 (TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell extracts were fractionated by SDS-PAGE transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad). After incubation with 3% BSA in TBST (10 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Mini-PROTEAN and Mini Trans-Blot system (Biorad). Total proteins on the membrane were stained with MemCode reversible staining kit (Thermo-Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with appropriate secondary antibodies conjugated with Alexa488 fluorophore and imaged using the ChemiDoc system (Bio-Rad). The dot intensities were quantified with the Image Studio Lite software (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2021Quote: Recombinant or mouse-extracted tau fibrils were diluted in PBS as indicated in Supplementary Figure 1 and applied to 0.2 µm nitrocellulose membrane using the Bio-Dot microfiltration apparatus (Bio-Rad). The membranes were then blocked in 5% milk TBS-Tween 20 and subsequently incubated with primary antibody overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were separated on 10 or 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked with Tris-buffered saline with Tween20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Five to ten microliters of each sample were subjected to native or SDS-PAGE and transferred into PVDF or nitrocelulose membrane (BioRad). Mouse monoclonal antibodies against β-actin ...
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bioRxiv - Physiology 2021Quote: ... membrane-enriched fraction: 5 μg per lane) were loaded onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA) – alternating between control and high CO2 treatments to avoid possible gel lane effects ...
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bioRxiv - Physiology 2021Quote: ... and the separated proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... the NC membrane was rewashed three times with PBST and imaged with ECL Western blotting detection system (Biorad, Cat# 1705062).
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting was performed with 4-15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad, Hercules, CA). Membranes were blocked with 5% milk in TBS with 0.1% Tween 20 (TBST ...
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bioRxiv - Immunology 2020Quote: ... and 30 μg of total protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). Correct protein loading was confirmed by Ponceau staining ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved using 4-12% BisTris-PAGE and transferred to a PVDF membranes (Bio-Rad catalogue number 162-0177) using a Gel Transfer Cell (BioRad catalogue number 1703930) ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Immunology 2021Quote: ... It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were then blocked with Tris-buffered saline with Tween20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was visualized with super signal west pico chemoluminescent substrate using the ChemiDoc™ MP imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... gels were subjected to a western blot in which protein was transferred onto a 0.45 μm nitrocellulose membrane using a Mini Trans-Blot Cell (Bio-Rad), in transfer buffer in the presence of an ice block for 1 hour at a constant current of 255 mA ...
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bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 13 000 g for 10 min and resolved using SDS-PAGE gels and transferred onto Polyvinylidene difluoride membranes (PVDF; BioRad). The membranes were blocked with 5% milk in TBST and probed with the indicated primary antibodies in 5% BSA/TBST and a 1:10000 dilution of anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies in 5% BSA/TBST ...
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bioRxiv - Bioengineering 2020Quote: ... after which proteins were transferred to polyvinylidene fluoride (PVDF) membranes (catalog no. 162-0177; Bio-Rad Laboratories; Hercules, CA, USA). Membranes were blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by SDS-PAGE (10-12% acrylamide) and transferred onto a PVDF membrane using the semidry TransBlot system (BioRad). The membrane was blocked with either 5% skimmed milk or 5% BSA (Bovine Serum Albumin ...