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Citations for Bio-Rad :
7201 - 7250 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Samples were run in 3-12% Bis-Tris gradient gels and transferred to PVDF membranes in a wet blot system (BioRad). Membranes were dried ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to membrane under 2.5A/25V condition for 7min using a semidry transfer system (Trans-Blot Turbo, BIO-RAD). Transferred membranes were first blocked by TBS-Tween20 (0.1% ...
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bioRxiv - Neuroscience 2021Quote: ... The sample was subject to SDS-polyacrylamide gel electrophoresis SDS-PAGE (7.5%) and then transferred to a polyvinylidene fluoride membrane (Bio-rad). After blocking in TBS-T (TBS with 0.05% Tween-20 ...
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bioRxiv - Cell Biology 2021Quote: ... 20-30 μg of total proteins from each sample were separated by SDS/polyacrylamide gel electrophoresis in running buffer (25 mM Tris pH 8.3, 192 mM Glycine, 0.1% SDS) and transferred onto nitrocellulose (NC) membranes (Bio-Rad, 1620094) in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membranes and blocked in TBS with 0.1% Tween-20 (TBST) and 5% Blotting Grade Buffer (BGB, Bio-Rad). The anti-RBM20 rabbit polyclonal primary antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... transferred onto PVDF membranes using Trans Blot Turbo Transfer System with RTA Transfer Packs (Bio-Rad Laboratories, Hercules, CA, USA) according to manufacturer protocol and analysed by immunoblotting using antibody-specific protocols ...
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bioRxiv - Microbiology 2021Quote: ... NC-membrane (pore size 0.45 mm) was used for protein transfer using Bio-Rad Mini PROTEAN blotting system (Bio-Rad). Proteins were transferred for 1 h at 300 mA in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... depending on the proteins analysed) and transferred on 0.2 μm nitrocellulose membranes (Trans-Blot® TurboTM Transfert Pack, Bio-Rad). Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau ...
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bioRxiv - Cell Biology 2022Quote: ... as well as respective inputs (20 µl of extracts corresponding to 50 µg of total proteins) were separated using SDS PAGE electrophoresis and transferred onto nitrocellulose membrane by electro-blotting (SemiDry, BioRad). Western blots were probed with rabbit anti-GFP (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... dissolved in dH2O) onto a nitrocellulose membrane (25V, 2.5A, 30 minutes) using a Transblot® Turbo Transfer system (Bio-Rad). The membrane was incubated in blocking buffer for 2 hours ((5% BSA in TTBS (NaCl ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... transferred to PVDF membrane (Amersham Hybond 0.2 um PVDF) at 100 V for 1hr 10 min with mini-protean tetra system (Bio-Rad) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... were neutralized by adding an equal volume of 2 M ammonium acetate (pH 7.0) and vacuum-transferred to a pre-washed nitrocellulose membrane using a BioDot SF microfiltration apparatus (Bio-Rad). Each well was washed 2 times with SSC buffer ...
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bioRxiv - Systems Biology 2022Quote: ... Protein extracts derived from OBC1 cells were electrophoretically transferred onto nitrocellulose membranes using a Trans-blot Turbo transfer system (up to 25V, 7min) (BioRad). Membranes were probed with primary antibodies at 1:1000 dilution in 5% nonfat milk or BSA according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The proteins were then transferred to a nitrocellulose membrane on a Trans-Blot® Turbo (Bio-Rad; Hercules, CA, USA). The membrane was blocked in a PBS solution containing 5% non-fat milk and 0.1% Triton X-100 for 1 h with orbital agitation ...
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bioRxiv - Neuroscience 2022Quote: ... 15 μg of protein was ran via SDS-PAGE in 10% Tris-tricine gels using BioRad protean mini and then rapid transferred to PVDF membranes using BioRad Semi-dry (BioRad). Membranes were subsequently blocked using 5% BSA in 1X TBST for one hour and then incubated with either 1° (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred to polyvinylidene difluoride membrane (Milipore, Billerica, MA, USA) using a semi-dry chamber (Trans blot SD semi-dry transfer cell, Biorad) and blocked with 4% semi-skimmed milk in PBS (blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated via SDS-PAGE in 15% acrylamide gels and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo Transfer System (BioRad) and PVDF transfer packs (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein samples were loaded on 10%-12% polyacrylamide gels and transferred onto 0.45 µm pore-size nitrocellulose membranes (Bio-Rad) using the Turboblot (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... transferred onto PVDF membranes using Trans Blot Turbo Transfer System with RTA Transfer Packs (Bio-Rad Laboratories, Hercules, CA, USA) according to manufacturer’s protocol and analysed by immunoblotting using antibody-specific protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and proteins were transferred to a PVDF membrane by using semi-dry Trans-Blot Turbo Transfer System (BioRad Laboratories, USA). Then the membranes were transferred into blocking solution (10% BSA or skimmed milk powder in PBS with 0.1% Tween20) ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was soaked with Chemi-Lumi Super (Nakarai Tesque) for the signal detection using ChemiDoc XRS+ (Bio-Rad Laboratories).
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bioRxiv - Genomics 2022Quote: Slot blot was performed using 1.5 ng of gDNA that was denatured in 400 mM NaOH/10 mM EDTA and blotted onto nitrocellulose membrane (BioRad) in duplicate for dsDNA and 5mC DNA using a slot blot apparatus (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were resolved by SDS-PAGE according to standard protocol and transferred onto 0.45μm nitrocellulose membranes (Bio-Rad 1620115) and blotted with the primary and secondary antibodies as described.
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bioRxiv - Cell Biology 2022Quote: ... 15 ug of mitochondrial proteins or 25 ug of total cellular proteins were separated by gel electrophoresis on 4-20% gradient TGX gels and transferred onto PVDF or nitrocellulose membranes using Trans-Blot Turbo Transfer system (BioRad). Mitochondrial electron transport chain proteins were detected using Total OXPHOS Rodent WB Antibody Cocktail (ab110413) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 % [w/v] Coomassie R) staining solution or blotted on polyvinylidenfluorid (PVDF) membranes using the Trans-Blot Turbo Transfer System (BioRad). As positive controls purified FtsZ1 or FtsZ2 was loaded on the respective gel ...
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bioRxiv - Microbiology 2020Quote: ... After electrophoresis the separated proteins were electroblotted onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) with a standard protocol for seven minutes ...
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bioRxiv - Developmental Biology 2021Quote: Protein samples were loaded on SDS-PAGE gel and then were transferred to Nitrocellulose membrane (Bio-Rad, Cat. Nr. 1620112). After blocking for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gel and then transferred to a nitrocellulose membrane (BioRad, Boston, MA, USA). The membrane was incubated with the appropriate primary antibody and then incubated with a species-appropriate secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µg of RNA isolated from the strain was spotted onto a Nytran membrane using a dot-blot apparatus (Biorad) and UV cross-linked (1200 energy ...
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bioRxiv - Molecular Biology 2020Quote: ... nuclear or histone extracts were resolved by SDS-PAGE and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (BioRad). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Immunology 2020Quote: The VSGm protein samples were separated on 10% SDS-PAGE then trans-blotted onto two separate nitrocellulose membranes using the mini PROTEAN II Trans-blot unit (Biorad). The membranes were rinsed in 1x PBS (0.1% w/v ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Plant Biology 2021Quote: ... 20 μg total protein was separated on 10% SDS-PAGE gels and blotted onto Immun-Blot PVDF membrane (Bio-Rad). LSD1-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from Bis-Tris gels to polyvinylidene fluoride (PVDF) membranes for 2 hours using a wet transfer system (BioRad). Following transfer ...
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bioRxiv - Microbiology 2020Quote: ... brucei were separated by SDS-PAGE (8% or 10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) (48) ...
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bioRxiv - Biochemistry 2020Quote: ... samples corresponding to equal amounts of protein were separated on a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane by the wet tank blot method (BioRad, according to manufacturer’s instructions) ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... Twice the amount of lysate was loaded onto gels for use in Western blotting and proteins were transferred to pre-equilibrated polyvinylidene difluoride (PVDF) membranes (BioRad) at 20 volts for 60 minutes using the BOLT transfer system (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred with PVDF membrane (Trans Blot Turbo, Cat.#1704157, Biorad). Membranes are blocked in %5 Milk with Tris-buffered saline with 0.1% Tween 20 detergent (TBST) ...
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bioRxiv - Cell Biology 2022Quote: ... Transfers onto PVDF or Nitrocellulose membrane were conducted using either a Trans-Blot Turbo system (with Turbo transfer buffer) (BioRad) or by traditional wet tank transfer using CAPS Buffer with 10% Methanol (for DNA-PKcs ...
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bioRxiv - Pathology 2022Quote: ... and transferred to a PVDF membrane (Trans-blot Turbo mini-Format 0.2 μM PVDF, cat.170-4156 by BIO-RAD). The membrane was blocked with 5% milk in PBS-T buffer (137mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Twenty micrograms of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immun-Blot, Bio-RAD, USA). After being blocked with 5% nonfat dry milk in Phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... the proteins from unstained gel were transferred to a nitrocellulose blotting membrane using Trans-Blot turbo Transfer System (Bio-Rad). The membrane was blocked with 5% skim milk in Tris buffered saline (TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 or 12.5% bis-tris gels and protein was transferred to PVDF membranes using a wet transfer system (Bio-Rad). Membranes were blocked with 5% milk in TBS-T and incubated overnight with primary antibody in either milk or BSA at manufacturer recommended concentrations ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad). Membranes were blocked overnight at 4°C in 3% (w/v ...