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Citations for New England Biolabs :
4301 - 4350 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... These Promoters were amplified by PCR and individually inserted by Gibson assembly (#E2611S; NEB) into the Downstream assay vector ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was performed using the OneTaq-Quick-Load 2X Master Mix (New England BioLabs) with Aedes whole-body cDNA as a template ...
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bioRxiv - Immunology 2021Quote: ... CD4 was amplified by PCR using Q5 DNA Hot Start Polymerase (New England BioLabs) in 0.2µM dNTP ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed using the LongAmp® Taq PCR Kit (New England Biolabs). Amplification conditions ...
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bioRxiv - Developmental Biology 2021Quote: The PCR product was digested with the restriction enzyme BsaI (New England Biolabs, R0535L) to yield a 168 bp fragment for the wild-type allele and a 120 bp and a 48 bp fragments for the mutant allele ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This 20 μL 10-cycle PCR reaction was performed using Q5 polymerase (NEB M0491L) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... The gel-purified PCR fragments were incubated with HiFi DNA assembly enzyme mix (NEB) and transformed into E ...
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bioRxiv - Developmental Biology 2020Quote: ... Genotyping-PCR was performed with Q5® High-Fidelity DNA Polymerase (New England Biolabs) with 98 °C initial denaturation for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... PCR product was then assessed using a ClaI restriction enzyme digest (New England BioLabs) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR mutagenesis for generating new BASIC parts were carried out using Phusion Polymerase (NEB) and phosphorylated oligonucleotides (IDT) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and the digestion product was purified using Monarch PCR & DNA Cleanup Kit (NEB, T1030S). Following the preparation of both the backbone vector and insert ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA purification was performed using the Monarch PCR & DNA Cleanup Kit (NEB, Ipswich, USA). Libraries were then amplified and purified before being size selected using magnetic AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: RNPS1 point and deletion mutants were PCR amplified using Q5 polymerase (New England Biolabs) and inserted into PB-CuO-MCS-BGH-EF1-CymR-Puro (modified from System Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: For cloning purposes all PCRs were done using Q5 high-fidelity DNA polymerase (NEB) and Gibson Assembly was performed using NEBuilder HiFi DNA assembly master mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat.# M0491L). PCR fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... and were PCR-amplified using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493) with 18 cycles.
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bioRxiv - Molecular Biology 2021Quote: ... PCR was done using Taq Polymerase according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
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bioRxiv - Genomics 2019Quote: ... The linearized fragments were PCR amplified with LongAmp Taq DNA Polymerase (New England Biolabs) and purified using 1x AmpureXP beads ...
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bioRxiv - Immunology 2021Quote: ... gBlocks were amplified by polymerase chain reaction (PCR) using Q5 polymerase (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was carried out using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 μL of NEB Next High-Fidelity PCR Master Mix (2×) (NEB, Ipswich, USA), 1 μL of Universal PCR Primer (25 mmol ...
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bioRxiv - Microbiology 2019Quote: ... Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs GmbH; Ipswich, USA), or innuPREP DOUBLEpure Kit (Analytik Jena ...
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bioRxiv - Immunology 2019Quote: ... PCR product was cloned into 5-alpha competent bacteria (New England Biolabs, cat. #C2987) using a TOPO TA cloning kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were fragmented randomly with the NEBNext® dsDNA fragmentase kit (NEB) and then purified with the AMPure® XP Beads kit (Beckman Coulter ...
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bioRxiv - Physiology 2019Quote: ... PCR was performed using Phusion Hot Start Flex 2× Master Mix (NEB, Frankfurt, Germany) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... The miR167-resistant ARF8 construct (rARF8) was generated by PCR site-directed mutagenesis (NEB) of the gARF8 entry clone ...
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bioRxiv - Genetics 2020Quote: ... PCR amplicons spanning both BtMFS guide recognition sites were generated using Q5 polymerase (NEB) with primers BtMFS_5primeF and BtMFS_exon2R (Supplementary Table 4) ...
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bioRxiv - Microbiology 2019Quote: The gel extracted DNA was amplified using the NEBNext PCR Master Mix (NEB#M0541S) with custom primers (WSO398 and WSO381 ...
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bioRxiv - Molecular Biology 2019Quote: ... If noted PCR products were used to synthesize RNA using T7 polymerase kit (NEB), purified using NEB RNA purification kit and equal amount of purified RNA was used for in vitro reaction (1 - 3ug) ...
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bioRxiv - Neuroscience 2019Quote: ... PCR product was digested using the restriction endonuclease HhaI (New England Biolabs, Ipswich, MA). For this 0.5μl of enzyme ...
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bioRxiv - Microbiology 2019Quote: ... the pchE gene was amplified in two overlapping PCR fragments using Phusion polymerase (NEB). Fragment 1 was amplified using the primers pchE 5’.2 and pchE int R and fragment 2 was amplified with primers pchE int 1B F (see Table S4 for primer sequences) ...
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bioRxiv - Neuroscience 2019Quote: ... The RBM45 cDNA was amplified by PCR using Phusion High-Fidelity DNA Polymerase (NEB) and sub-cloned into the pcDNA3 vector (Invitrogen) ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... The PCR reaction was performed with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) and the following protocol ...
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bioRxiv - Immunology 2019Quote: PCR amplicons were amplified with the Phusion high-fidelity DNA polymerase (NEB, Ipswich, MA) using manufacturer recommended cycling conditions ...
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bioRxiv - Microbiology 2019Quote: Genes of interest were amplified by PCR amplification using Phusion-HF DNA Polymerase (NEB). Sequences were inserted into pBAD18 or pBAD33 by restriction digest using EcoRI ...
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bioRxiv - Microbiology 2021Quote: ... The PCR fragments were stitched together using the Gibson assembly kit (NEB CN# E2611S) following manufacturer’s instructions to yield pJV204 ...
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bioRxiv - Genomics 2020Quote: ... PCR products were treated with 1 μl of exonuclease (New England Biolabs, 20 units), and the mixture was then incubated at 37°C for 15 min ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were treated with exonuclease I and alkaline phosphatase (NEB, M0293; M0371) and Sanger sequenced to verify successful knockouts ...
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bioRxiv - Genomics 2020Quote: ... Libraries were amplified using NEBNext® High-Fidelity 2X PCR Master Mix (#M0541S, NEB) with primers Ad1 ...
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bioRxiv - Immunology 2021Quote: ... 25 μl NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, Cat. #M0541L) per reaction and amplified via a 6-cycle PCR program (1 cycle of 72°C for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... were then purified with a Monarch® PCR & DNA Cleanup Kit (5μg) (NEB T1030S) and DNA concentration and purity measured by NanoDrop™ spectrophotometry (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR was done using Taq Polymerase according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: ... The PCR products were subcloned into pcDNA3.1(-) vector using NEBuilder HiFi DNA Assembly (NEB) to express the recombinant proteins tagged with HA at C-terminus (pcDNA3.1(-)-FL-CatSper1-HA and pcDNA3.1(-)-ND-CatSper1-HA.
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed according to the manufacturer’s instructions using Q5 Hot Start Polymerase (NEB) using the following program ...
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bioRxiv - Bioengineering 2020Quote: ... PCR 1 conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP2_KO_A and clpP2_KO_D primer pair (Table 3 ...