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Citations for New England Biolabs :
4251 - 4300 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 29 μl of PCR master mix (2x Q5 high-fidelity master mix (NEB), 1 μM 2P universal forward primer ...
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bioRxiv - Systems Biology 2023Quote: PCR amplifications were performed using Phusion High-Fidelity DNA polymerase (New England Biolabs) and oligonucleotides for cloning were obtained from Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were cloned into target constructs using HiFi Master mix (E2621, NEB). All constructs were verified by dideoxynucleotide sequencing (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR was performed using Q5 2× Master Mix (New England Biolabs, M0492S), 6 µL of cDNA synthesis reaction ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl NEBNext High-Fidelity 2X PCR Master Mix (NEB, catalog number M0541S). In each 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs), restriction digestion analysis and Sanger sequencing in order to confirm the clones (Table S6 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed at 30 cycles using Q5 High fidelity DNA Polymerase (NEB) at 0.02 U/µl according to further manufacturer’s instructions ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... PCR fragments were processed using the NEBuilder Hifi DNA assembly kit (NEB #E2621S) using the steps described above.
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bioRxiv - Cancer Biology 2023Quote: The coding sequence of bwCIRBP was amplified by PCR using Phusion polymerase (NEB), digested with EcoRI and NotI ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were gel purified with the Monarch Gel Extraction Kit (NEB, T1020).
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bioRxiv - Cell Biology 2023Quote: ... All PCRs were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion® High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs) was used to screen C ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplification was performed using Q5® High-Fidelity DNA Polymerase (NEB #M0491L). The original vector was digested with the restriction enzyme DpnI (NEB #R0176S ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR fragment was digested with EcoRI (New England Biolabs, Cat# R3101S) and BamHI (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... using Q5 High-Fidelity PCR master mix with HF buffer (NEB Ipswich, MA) and PCR primers containing the T7 promoter sequence (Forward ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed with Q5U HotStart High-Fidelity polymerase (New England Biolabs, M0515) as usual except a custom dNTP mix was used that contained dATP ...
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bioRxiv - Genomics 2024Quote: ... PCR amplification with was Q5® High-Fidelity DNA Polymerase (New England Biolabs) with CFTR Exon 8 forward primer (5’-TTTCTGTTGGTGCTGATATTGCCTCAGGGTTCTTTGTGGTGT-3’ ...
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bioRxiv - Bioengineering 2024Quote: ... Genotypes were then confirmed by colony PCR with OneTaq (New England Biolabs, UK) and selected for antibiotic sensitivity ...
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bioRxiv - Genomics 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB). The first-dimension electrophoresis was run in 0.7% agarose in 1xTBE buffer at 0.7 V/cm for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... which were generated via PCR using the Q5 DNA Polymerase (New England BioLabs), purified with HighPrep PCR Clean-up beads (MagBio) ...
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bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragments were amplified using Thermo Scientific Phusion High-Fidelity DNA Polymerase (NEB) and ligated into entry vectors through BsaI-restriction cloning ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was done with the Q5® High-Fidelity 2X Master Mix (NEB). After PCR1 in the nested PCR strategy ...
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bioRxiv - Cell Biology 2024Quote: ... The 3’ homology arm was amplified by PCR (Q5 HiFi polymerase®, NEB), from HCT116 cell genomic DNA using the primers NCAPH2 3’ homology arm forward and reverse ...
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bioRxiv - Immunology 2024Quote: ... promoting amplification of this region via Q5 polymerase-based PCR (New England Biolabs). PCR products were then identified by agarose gel method and purified via Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Biochemistry 2024Quote: All PCR reactions for genotyping were performed with the OneTaq DNA Polymerase (NEB) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments and the linearized pITR2 vector were digested with SfiI (NEB, R0123S), purified ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB), and RNA was synthesised from the purified DNA using the MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... purified PCR products were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB). Assembled gRNA/Cas9 and donor plasmids were transformed into DH5α bacteria and final purified plasmids were checked by sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Genetics 2024Quote: ... The PCR-amplified oligo pool was then digested with BsaI-HFv2 (NEB #R3733S), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Mutagenic PCR products were then digested with 10 U DpnI (New England Biolabs) at 37°C for 30 min to eliminate template DNA ...
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bioRxiv - Cell Biology 2024Quote: All PCR amplification was completed using Q5 High-Fidelity DNA Polymerase (NEB #M0491S) for all PCR amplifications including site-directed mutagenesis.
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR reaction mix was created with NEB Phusion GC Buffer (NEB, B0519S), Phusion DNA Polymerase (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... Quantitative PCR was performed with Luna Universal qPCR Master Mix (New England BioLabs) using an Applied Biosystems ViiA 7 Real-Time PCR system ...
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bioRxiv - Systems Biology 2024Quote: ... 28 cycles) using the Phusion® High-Fidelity PCR Master Mix (NEB, M0531L). Samples were verified using gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: ... We used Phusion High-Fidelity PCR MasterMix with HF Buffer (NEB, Catalog #M0531L) for the second PCR and used the following PCR conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Selections were then purified using the Monarch® PCR & DNA Cleanup Kit (NEB), eluting in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Genomics 2024Quote: ... followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®). Standard experimental conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR was performed using the Luna Universal qPCR Master Mix (NEB, M3003L) in a BioRad machine ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR product was gel extracted with the Monarch DNA Gel Extraction Kit (NEB). 20,000 HEK-293FT cells were plated into 96 well plates ...
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bioRxiv - Biophysics 2019Quote: ... the 5’ ends of the PCR products were phosphorylated by T4 polynucleotide kinase (NEB), the products were purified and the free ends of the plasmid re-ligated by T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were end-repaired with T4 DNA polymerase (NEB; Cat. No. B02025), DNA Polymerase I ...
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bioRxiv - Microbiology 2020Quote: ... Three PCR fragments were produced using the Q5 DNA polymerase system (New England Biolabs) according to the manufacturer’s instructions (54) ...
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bioRxiv - Microbiology 2020Quote: ... was made by mutagenesis PCR using the site-directed mutagenesis kit (New England Biolabs). Additional mutants of this intermediate vector were generated through site-directed mutagenesis of the HHRz sequence ...
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bioRxiv - Cell Biology 2020Quote: All PCRs for cloning procedures were performed using NEB Phusion polymerase (New England Biolabs). Primers used for cloning ...
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bioRxiv - Genetics 2021Quote: ... PCR was used to amplify DNA fragments and Gibson assembly cloning (New England Biolabs) employed to assemble fragments together ...