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Citations for New England Biolabs :
4201 - 4250 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids and HaloTag PCR products were digested using AvrII (New England Biolabs, R0174S) and MluI (NEB ...
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bioRxiv - Biophysics 2023Quote: ... and the destination PCR-fragment was DPN1 treated (R0176, New England Biolabs, UK). These two fragments where ligated together using T4 DNA ligase (M0202 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were gel purified with the Monarch Gel Extraction Kit (NEB, T1020).
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bioRxiv - Cell Biology 2023Quote: ... PCR products were gel purified with the Monarch Gel Extraction Kit (NEB, T1020).
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bioRxiv - Cell Biology 2023Quote: ... All PCRs were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... The libraries were then purified using the Monarch PCR & DNA Cleanup Kit (NEB), using a 5:1 ratio of Binding Buffer ...
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bioRxiv - Genetics 2023Quote: ... we used the Q5® High-Fidelity PCR Kit (New England Biolabs, E0555S) with the following conditions ...
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bioRxiv - Genetics 2023Quote: ... We used the Q5® High-Fidelity PCR Kit (New England Biolabs, E0555S) with the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... four PCRs were performed (Figure S1C) with Phusion polymerase (New England Biolabs, M0530S). We started by generating a linear DNA template to reduce bias in our PCR reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... A second round of PCR cycle (25 cycles with the Phusion from NEB) was performed to add the Illumina adapters containing the different indexes ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was carried out using Q5® High-Fidelity DNA Polymerase (NEB) for at least 35 cycles ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions were performed as recommended by the manufacturer (New England Biolabs) with optimized annealing temperatures as indicated in Supplementary Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) for insert-plasmid assembly ...
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bioRxiv - Microbiology 2023Quote: ... for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs, NEB) for insert-plasmid assembly ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were digested overnight with DpnI (NEB, Frankfurt am Main, Germany) and transformed into Escherichia coli DH5α ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were generated with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) using 1 μl of the diluted lysate per 10 μl reaction volume ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... Each PCR contained 12.5μL Q5 High Fidelity 2x Master Mix (New England Biolabs), 1.1μL of pooled primers mixes at 10μM (final concentration of each primer was ∼10-11pM) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR fragments were digested with KpnI (New England Biolabs, Cat# R0142S) and NotI (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA PCR was performed with using OneTaq® 2X Master Mix (NEB) and used primer pairs were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR mixtures used Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the specified primers and templates below ...
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bioRxiv - Microbiology 2023Quote: ... The gene of interest was amplified by PCR with Phusion (New England Biolabs), digested with with BamHI and XbaI (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... The product was column-purified with Monarch® PCR & DNA Cleanup Kit (NEB) and digested with BbsI ...
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bioRxiv - Genetics 2023Quote: ... Amplification was performed with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541s) and Nextera PCR Primers (8 cycles) ...
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bioRxiv - Genomics 2023Quote: ... The PCR was performed using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) with conditions described in Supplementary Table 4.
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491S) and primers harbouring overhangs for Gibson Assembly ...
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bioRxiv - Biophysics 2023Quote: ... and Q5 Hot Start High-Fidelity 2X PCR Master Mix (New England Biolabs). The transcribed region had the following spacings ...
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bioRxiv - Biochemistry 2023Quote: ... was produced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR product obtained was digested by methylation-specific enzyme DpnI (New England Biolabs) and transformed into bacteria for obtaining isolated colonies ...
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bioRxiv - Genomics 2022Quote: ... The resulting fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2023Quote: ... The PCR mixture composed 12.5 μL of 5X LongAmp Taq Reaction Buffer (NEB), 0.4uM of forward and reverse primers (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: All dsRNA templates were PCR-amplified using Q5 high-fidelity DNA polymerase (NEB) from O ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed using Q5 Hot-start 2× master mix (New England Biolabs). Restriction digests were conducted for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed using the Q5 ® Hot Start Master mix (NEB M0491), supplemented with 10 μΜ of each primer and 20 ng of template in a final volume of 25 μl in the following conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative PCR was performed with Luna® Universal qPCR Master Mix (NEB #M3003) and Biorad CFX96 Real time PCR system ...
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bioRxiv - Genomics 2023Quote: ... Both PCR rounds were performed with Q5-High Fidelity polymerase (New England Biolabs) for 6 and 11 cycles ...
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bioRxiv - Biophysics 2023Quote: ... coli (MG1655) genome containing the rrnC operon was amplified by PCR (Phusion, NEB) using the following primers:
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bioRxiv - Genomics 2023Quote: ... The pETv8 backbone and PCR products were digested with BamHI restriction enzyme (NEB) and gel purified ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification was performed with a Q5 HotStart High Fidelity Polymerase (NEB, #M0493S), and resulting blunt-ended PCR products were cloned into a pCR-Blunt II-TOPO vector (Invitrogen Zero Blunt TOPO PCR Cloning Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... the One Taq HS QuickLoad PCR reagent was used (Catalog no. M0488L, NEB). The genotyping process utilized the primer sets and cycling conditions listed in Supplementary Table 1.
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bioRxiv - Plant Biology 2023Quote: ... Reactions were purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs) and sent to Source BioScience ...
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bioRxiv - Genetics 2024Quote: ... All PCRs were performed using LongAmp Taq DNA polymerase (NEB, cat no. 50994936) in a 25 μL reaction volume according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... The digests were then purified using a Monarch PCR&DNA Cleanup Kit (NEB) and amplified using firstly a nested PCR reaction with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Following DpnI digest and PCR purification ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl NEBNext High-Fidelity 2X PCR Master Mix (NEB, catalog number M0541S). In each 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs), restriction digestion analysis and Sanger sequencing in order to confirm the clones (Table S6 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were cloned into target constructs using HiFi Master mix (E2621, NEB). All constructs were verified by dideoxynucleotide sequencing (Macrogen) ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... PCR fragments were processed using the NEBuilder Hifi DNA assembly kit (NEB #E2621S) using the steps described above.
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed at 30 cycles using Q5 High fidelity DNA Polymerase (NEB) at 0.02 U/µl according to further manufacturer’s instructions ...