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Citations for New England Biolabs :
3501 - 3550 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and DNA cleaned with Monarch PCR & DNA Cleanup Kit (NEB T1030S). Resulting supercoiled plasmid is linearized at the 3’ end of the PolyA tail using BamHI-HF (NEB R3136S) ...
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bioRxiv - Genetics 2020Quote: ... PCR was conducted using OneTaq 2x Master Mix (New England Biolabs) with forward and reverse primers following the program of 95°C×10min ...
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bioRxiv - Biophysics 2021Quote: ... a 2.4 mL PCR reaction was performed using Taq polymerase (NEB) in 1X GoTaq buffer to amplify a 25-N-43 DNA ...
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bioRxiv - Microbiology 2021Quote: ... the two fragments were generated using Phusion PCR (New England Biolabs) by amplifying the TurboGFP open reading frame from the vector pGIPZ (Thermo Scientific Open Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Circular PCRs were performed with a high-fidelity polymerase (Q5, NEB) followed by digestion with DpnI restriction enzyme (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was amplified by PCR (Q5 High-Fidelity DNA Polymerase, NEB) using primers (RTWhitelist_Fwd/Rev ...
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bioRxiv - Synthetic Biology 2022Quote: ... All PCRs were performed with Phusion Polymerase (New England Biolabs, USA). All constructs were verified by Sanger sequencing (Microsynth AG ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were quenched with Monarch PCR & DNA Cleanup Kit (NEB T1030S) and the purified DNA eluted in 20 µL EB buffer ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... Overlapped PCR fragments were generated (Phusion High-Fidelity DNA Polymerase, NEB) and cloned into competent cells with standard In-Fusion HD cloning techniques ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S). The assembled plasmid library was electroporated into electrocompetent bacteria (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S) and used to electroporate into electrocompetent bacteria (NEB ...
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bioRxiv - Biophysics 2022Quote: ... PCR was performed using a LongAmp DNA polymerase (New England BioLabs) following the protocol from the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product and pUltra were digested with XbaI (NEB# R0145S) and BamHI (NEB# R3136T ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed using LongAmp Taq 2X Master Mix (NEB M0287S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The eluate was PCR amplified using 2x NEBNext Master Mix (NEB) and pre-mixed primers with unique dual indexes for Illumina sequencing (IDT) ...
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bioRxiv - Microbiology 2022Quote: ... We used two high fidelity PCR systems—Q5 (NEB cat. M0492), and Platinum SuperFi II (Invitrogen cat ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reaction (PCR) was performed with Phusion DNA polymerase (NEB) in a total volume of 50 µl in GC buffer containing 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid-backbone PCRs were restriction digested with DpnI (New England Biolabs) following the manufacturers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and plasmids were purified with Monarch DNA kits (NEB). Overlapping primers for amplification were designed using the NEBuilder assembly tool (https://nebuilderv1.neb.com/ ...
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bioRxiv - Immunology 2022Quote: ... All PCRs were performed using Q5 High Fidelity DNA Polymerase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were digested with DpnI (New England Biolabs, MA, USA) as indicated by the manufacturer and purified using the EZNA Cycle Pure Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was carried out with phusion polymerase (New England Biolabs) according to instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Library was PCR amplified with unique indexes (E7335S, New England Biolabs) for 14 cycles ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were digested with the AvrII (CCTAGG) (NEB, Beverly, MA) restriction endonuclease ...
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bioRxiv - Plant Biology 2022Quote: ... All PCRs were carried out using Q5 high fidelity polymerase (NEB). NEB Stable Competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions contained 5X Q5 reaction buffer (New England Biolabs, UK), Q5® High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions contained 5X Q5 reaction buffer (New England Biolabs, UK), Q5® High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... We performed secondary PCR to add TruSeq barcodes (NEB Q5 HotStart). Libraries were sequenced as paired end 2 × 250 read multiplex runs on a MiSeq instrument.
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bioRxiv - Plant Biology 2022Quote: ... PCR products were digested with DpnI (New England Biolabs; Ref.: R0176S) to remove circular DNA ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genetics 2023Quote: ... PCR fragments were amplified using Phusion DNA polymerase (New England Biolabs) using the generic protocol and 30 s/Kb extension ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
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bioRxiv - Genetics 2024Quote: ... DNA was PCR amplified with Q5 high fidelity DNA polymerase (NEB). The forward primer MitfaPCR-F binds to the mitfa genomic region upstream of the insertion site and the reverse primer 5’homology_amplify-R binds the p2A region of the insertion cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then amplified with Q5 PCR mix (New England Biolabs) for a total of 16-25 cycles ...
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bioRxiv - Systems Biology 2024Quote: ... We setup 50uL PCR reactions with 1x OneTaq GC Buffer (NEB), 400uM of additional MgSO_4 ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (NEB; M0491L). The amplicon was treated with KLD enzyme mix (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB). All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a post-PCR cleanup kit (New England Biolabs) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The third PCR was performed with the Phusion hot Start (NEB) to add the Illumina adapter and index ...
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bioRxiv - Genomics 2024Quote: ... Genomically integrated guide sequences were amplified using NEBNext PCR polymerase (NEB) and staggered guide-specific primers (Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was PCR-amplified using Phusion polymerase (New England Biolabs; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The PCR product was digested with SfiI and NotI enzymes (NEB) and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Duplex PCR was performed using Q5 Hotstart 2x Master Mix (NEB) with final primer concentrations of 0.5uM ...
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bioRxiv - Synthetic Biology 2023Quote: ... by reverse PCR and blunt end ligation with KLD (NEB M0554S). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-5 cycles of PCR with OneTaq polymerase (New England Biolabs) was performed using the forward (pBPS_fwr ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µL NEBNext HiFi 2× PCR Master Mix (New England BioLabs) was added to the DNA mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was treated with T4 PNK (NEB M0201S) and T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Cell Biology 2023Quote: ... gBlocks were PCR-amplified with Q5 High-Fidelity DNA Polymerase (NEB) using the forward primer T7-Kozak-start-fwd and the reverse primers HA-PolyA-rev (for PNPLA3-HA constructs ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were carried out using the Q5 DNA polymerase (NEB) on Arabidopsis cDNA libraries or genomic DNA ...