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Citations for New England Biolabs :
3451 - 3500 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... The PCR product was digested with the restriction enzyme HpyAV (NEB) to distinguish heterozygous and homozygous mutants.
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bioRxiv - Biophysics 2019Quote: ... 96 × 50 ul PCR reactions in 1 × ThermoPol reaction buffer (NEB) were prepared using template (0.01 ng µL-1) ...
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bioRxiv - Developmental Biology 2020Quote: ... PCRs were performed with Q5® High-Fidelity DNA Polymerase (NEB) for at least 30 cycles ...
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bioRxiv - Synthetic Biology 2020Quote: ... The PCR products were treated with DPN1 (New England Biolabs inc.) and purified using the Agencourt AMPure XP system ...
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bioRxiv - Molecular Biology 2019Quote: All PCR reactions were performed with Q5 polymerase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... was amplified by PCR using Phusion High-Fidelity DNA Polymerase (NEB). DNA fragments for the IDR of EWS (residues 47-266 ...
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bioRxiv - Microbiology 2021Quote: ... and purified using Monarch PCR clean-up kit (New England Biolabs). Blunted DNA was A-tailed using NEBNext dA-tailing kit and column-purified ...
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bioRxiv - Biochemistry 2020Quote: ... purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs, USA) and cloned into PET-24a (+ ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR fragments were digested with XhoI (New England BioLabs, USA) and NdeI (New England BioLabs ...
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bioRxiv - Neuroscience 2019Quote: ... cleaved fragments were amplified by PCR using Q5 DNA polymerase (NEB) and cloned in pCR™4Blunt-TOPO® vector according to manufacturer’s instruction ...
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bioRxiv - Genetics 2020Quote: ... and NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs) with 12 cycles ...
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bioRxiv - Genomics 2020Quote: ... 25 µl 2x NEBNext High-fidelity PCR mix (New England Biolabs), and 15 µl H2O ...
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bioRxiv - Molecular Biology 2020Quote: ... Polymerase chain reactions (PCRs) were performed using Phusion DNA polymerase (NEB) according to manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR product was transcribed using the T7 IVT Kit (NEB), followed by DNase I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB, T1030L). The source of the 3xHA-Nluc starting CDS (“Nluc start” ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by purification with Monarch® PCR & DNA Cleanup Kit (NEB). The Mobius Assemblies were verified first by colony PCR using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The 25□µL final reaction volume contained 1X PCR Buffer (NEB), 3□mM MgCl2 ...
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bioRxiv - Genetics 2020Quote: ... PCR fragments were amplified using Phusion polymerase (New England Biolabs M0530). Plasmids were digested with restriction enzymes at 37°C for 2-16hrs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All PCR amplification steps were done using Q5 polymerase (NEB, M049L) and TOP10 chemically competent E ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Physiology 2021Quote: ... Each PCR product was run alongside a 100bp DNA ladder (BioLabs) compared with positive and negative controls.
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bioRxiv - Microbiology 2021Quote: ... Purified PCR product was digested with BsaHI RE enzyme (NEB, UK), as per the manufacturer"s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA sequence was PCR amplified using Phusion enzyme (NEB, M0530S) and appropriate primers with overhangs for Not1 and Kpn1 restriction enzymes (sequences given in Supplementary Table 2) ...
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bioRxiv - Evolutionary Biology 2020Quote: PCR was performed on cDNA using Q5 High-Fidelity Polymerase (NEB). The K1 ORF was amplified using primers M1_F1 or M1_F5 and M1_R6 (Table S3) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments were amplified using Q5 polymerase (New England Biolabs, NEB). DNA fragments were isolated using the Qiaprep spin miniprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments were amplified using Q5 polymerase (New England Biolabs, NEB). DNA fragments were isolated using the Qiaprep spin miniprep kit (Qiagen) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were purified with Exo-CIP Rapid PCR Cleanup Kit (NEB) following supplier’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The 2810-bp PCR product was phosphorylated with polynucleotide kinase (NEB) and circularized with T4 DNA ligase (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... or the Exo-Cip Rapid PCR Cleanup Kit (New England Biolabs). Gene editing efficiency was analyzed using TIDE analysis software (Brinkman et al ...
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bioRxiv - Cell Biology 2021Quote: ... EJ5-GFP insertion was verified by PCR (OneTaq, New England Biolabs). CHO-K1 ATM+ was generated by transfecting a clonal population of CHO-K1 EJ5-GFP with a Cas9:tracrRNA:sgRNA ribonucleoprotein particle (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... all PCR products were generated with Phusion polymerase (New England Biolabs). All plasmids were sequence-verified.
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bioRxiv - Plant Biology 2022Quote: ... the amplified PCR products were digested using DpnI (New England Biolabs) and transformed into DH5a competent cells ...
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bioRxiv - Plant Biology 2022Quote: ... the amplified PCR products were digested using DpnI (New England Biolabs) and transformed into DH5a competent cells ...
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bioRxiv - Cell Biology 2021Quote: ... and used as DNA template for PCR with Phusion Polymerase (NEB) or Q5 Polymerase (NEB ...
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bioRxiv - Genetics 2021Quote: ... PCR fragments were amplified using Phusion polymerase (New England Biolabs (NEB), M0530 ...
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bioRxiv - Genetics 2021Quote: ... PCR fragments were amplified using Phusion polymerase (New England Biolabs (NEB), M0530 ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was PCR amplified with NEB Q5 HotStart polymerase (NEB) using splicing assay primers from IDT (AGACCCAAGCTGGCTAGCGTT forward ...
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bioRxiv - Immunology 2020Quote: ... PCR-amplified fragments were digested with XbaI and XhoI enzymes (NEB) and cloned into the NheI and SalI sites of pCW57-MCS1-P2A-MCS2 (Hygro ...
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bioRxiv - Immunology 2020Quote: ... PCR-amplified fragments were digested with NheI and SalI enzymes (NEB) and cloned into the NheI and SalI sites of a pCW57.1 vector ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were digested with restriction enzyme XmaI (New England Biolabs) and ligated with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR step was carried out by Taq DNA polymerase (NEB) using forward primer identical to the adapter sequence (Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... DNA was PCR-amplified using either Phusion (New England Biolabs; NEB), Pfu (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was PCR-amplified using either Phusion (New England Biolabs; NEB), Pfu (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... cleaned up using the Monarch PCR Purification kit (New England Biolabs), and used in a Gibson assembly reaction (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: PCR fragments were digested with BsaI or BsmBI restriction enzyme (NEB) to specific sticky end ...
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bioRxiv - Immunology 2020Quote: PCRs were performed with Q5 High-Fidelity DNA polymerase (NEB, M0491L) in 8 parallel 50ul reactions with the following cycle conditions ...
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bioRxiv - Microbiology 2021Quote: PCR amplicons and plasmids were purified using Monarch DNA kits (NEB). PCR ...
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An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolutionbioRxiv - Developmental Biology 2020Quote: ... and dla were amplified via high fidelity Phusion-HF PCR (NEB) from 48 hpf AB WT cDNA libraries using primers in the Key resources table ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified PCR product was then cut using restriction enzymes (NEB) and ligated to linearized vector pGex-6p-1 (GST ...
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bioRxiv - Cell Biology 2021Quote: ... fragments were PCRs amplified with Phusion DNA Polymerase (New England Biolabs) and were assembled by T4 ligase (New England Biolabs ...