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Citations for New England Biolabs :
3351 - 3400 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... by linearizing the vector with 15 base pairs (bp) overlapped primers using Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. MO531L). PCR products were digested using Dpn I (Takara ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... sticky ends were filled by incubating the nuclei for 4h at RT with DNA Polymerase I (New England Biolabs, Cat. N: M0210) and a biotin-14-dATP (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 minutes using NEB Quick T4 DNA Ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckmann Coulter ...
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bioRxiv - Microbiology 2019Quote: ... and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) for qPCR and RT-qPCR test ...
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bioRxiv - Plant Biology 2019Quote: ... Three different biological replicates represented by total RNA extracted from three individual plants and reverse transcribed into cDNA by LunaScript®RT SuperMix Kit (New England Biolabs Inc.) were used for statistical analysis of the quantification ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA integrity was validated by gel electrophoresis and 800 ng were reverse transcribed using the LunaScript® RT SuperMix Kit according to manufacturer’s instructions (E3010, New England Biolabs, Ipswich, MA). Real-time PCR was performed using the Blue ŚGreen qPCR Kit (F416 ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003, New England Biolabs, MA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
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bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 min using NEB Quick T4 DNA ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... as described by Corman et al84 were used alongside the Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswitch, MA) and CFX384 Touch Real-Time System (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred and fifty ng of RNA were used for reverse transcription reactions using the LunaScript RT SuperMix (New England BioLabs cat# E3010). For the qPCR ...
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bioRxiv - Immunology 2024Quote: ... cDNA for 3’ RACE was generated using RNA from IFN-β treated A549 cells using the Template Switch RT Enzyme Mix (NEB, #M0466) and anchored primers ...
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bioRxiv - Cancer Biology 2023Quote: ... 150 ng of total RNA per sample was used to synthesize cDNA using LunaScript RT SuperMix kit (Cat# E3010L, NewEngland BioLabs, MA, USA) in a 20 µl reaction volume ...
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bioRxiv - Biochemistry 2024Quote: ... DNA digestion was performed with DNase I and RNA was reverse-transcribed into cDNA using LunaScriptTM RT SuperMix kit (New England Biolabs, Cat#E3010L). qPCR was performed using the StepOnePlusTM Real-Time PCR System with SYBR green as the detection method ...
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bioRxiv - Cell Biology 2020Quote: ... The amplified PCR product was treated with DpnI (New England Biolabs) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: All PCRs were conducted using Q5 DNA polymerase (New England Biolabs) and the primers listed in Supplemental Table 1 ...
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bioRxiv - Immunology 2021Quote: ... PCR was conducted to amplify the whole sequence (Phusion polymerase, NEB), the amplicon was A-tailed and ligated into a T-tailed version of pICSL86955OD with T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried using Q5 Hot Start Polymerase (NEB, M0493S).
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bioRxiv - Genetics 2021Quote: ... Genomic sequences were PCR amplified using Q5 High fidelity Polymerase (NEB), purified Canton S genomic DNA (Gentra Puregene Tissue Kit ...
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bioRxiv - Genomics 2020Quote: ... and DNA purification after PCR was performed according to (NEB, E7645) using NEBNext® Multiplex Oligos for Illumina® ...
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bioRxiv - Genetics 2020Quote: ... Both were processed by an overlapping Phusion PCR (NEB, Ipswich, USA) and purified with Monarch PCR & DNA Cleanup Kit (5 μg ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were digested with BsiWI and NheI (New England Biolabs) then ligated into the genome of recombinant RABV strain B2c (rB2c ...
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bioRxiv - Immunology 2021Quote: ... 2.5 µl Evagreen and 25 µl Q5 PCR mix from NEB ultra II Kit (E7645S ...
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bioRxiv - Neuroscience 2022Quote: ... Genes of interest were amplified via PCR using Phusion Polymerase (NEB) and primers for pJET 1.2 with an overhang of Sp6 promoter ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification was performed using Phusion polymerase (New England Biolabs; NEB) unless otherwise noted ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification was performed using Phusion polymerase (New England Biolabs; NEB) unless otherwise noted ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were amplified using Phusion High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... and PCR-amplified using Phusion High-Fidelity DNA Polymerase (M0530S, NEB), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Colony PCR was performed using Q5 DNA polymerase (New England Biolabs) or KOD One (TOYOBO ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments were amplified by Q5 DNA polymerase (New England Biolabs) or KOD One (KMM-101 ...
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bioRxiv - Biophysics 2022Quote: ... and degradation tags were amplified by PCR with Q5 polymerase (NEB), gel extracted (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... and PCR amplified using the Q5 proofreading polymerase (New England Biolabs) without cloning into a plasmid ...
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bioRxiv - Developmental Biology 2022Quote: ... were PCR-amplified with adaptor sequences (IDT) and assembled (NEB; E5520) into the lentiviral backbone pTetOne (Takara ...
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bioRxiv - Genomics 2020Quote: ... For all PCR reactions the Phusion HotStart Flex DNA Polymerase (NEB) was used with an annealing temperature of 55°C ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were digested with DpnI restriction enzyme (New England Biolabs), used to transform competent bacteria and selected for positives ...
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bioRxiv - Molecular Biology 2019Quote: ... and subsequent PCR amplification of the cDNA was with Phusion (NEB). A step-by-step protocol for preparing small-RNA sequencing libraries is available at http://bartellab.wi.mit.edu/protocols.html ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR fragments were amplified using Q5 polymerase (New England BioLabs (NEB)) ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR fragments were amplified using Q5 polymerase (New England BioLabs (NEB)) ...
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bioRxiv - Systems Biology 2019Quote: ... PCR product was then subjected to DpnI digestion (NEB cat# R0176S) and gel purification with Zymoclean Gel DNA Recovery Kit (ZYMO cat#D4001) ...
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bioRxiv - Genomics 2020Quote: ... 25.0 μl of 2x NEBNext PCR Master Mix (NEB cat M0541); and 7.0 μl nuclease free H2O ...
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bioRxiv - Genetics 2020Quote: ... The PCR amplification was performed using Phusion high fidelity polymerase (NEB) and the amplified fragments were cloned in Gateway pDONR entry vectors (pP4P1r or pP2rP3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each PCR product was digested with restriction enzymes (New England Biolabs), gel purified (Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... DNA was purified using Monarch® PCR & DNA Cleanup Kit (NEB), using the following recommended protocol modification ...
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Melon ethylene-mediated transcriptome and methylome dynamics provide insights to volatile productionbioRxiv - Plant Biology 2020Quote: ... following insertion of the PCR product with NEBuilder (New England BioLabs). Growing media ...