Labshake search
Citations for New England Biolabs :
301 - 347 of 347 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... The Sox2(600bp)::GFP plasmid was generated by digesting the primer overhangs of the PCR product with XhoI and EcoRV (NEB) and its consequent ligation into linearised plasmid ISceI/MCS-d1GFP-SV40/ISceI ...
-
bioRxiv - Neuroscience 2023Quote: We amplified the full-length Ncan sequence by PCR from the mouse brain cDNA library and assembled it into the pCAG-GFP vector using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) or In-Fusion HD Cloning Kit (TaKaRa) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... flies and inserted into the XbaI and XhoI sites of pJFRC81-10XUAS-IVS-Syn21-GFP-p10 (addgene #36432) via Gibson assembly (NEB). The resultant plasmid was injected into the D ...
-
bioRxiv - Plant Biology 2024Quote: ... The Z. tritici codon-optimized GFP was amplified from pCeGFP (Kilaru et al. 2015) using NEB Phusion polymerase (New England Biolabs) and the primers listed in Supporting Information Supplementary Table S2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Base editing sgRNA directed against the SF3B1 K700 locus was designed using BE-Designer28 and cloned into pLKO.5 Puro-2A-GFP between BsmBI (New England Biolabs) cut sites ...
-
bioRxiv - Cell Biology 2024Quote: ... domain from pmEGFP-N1-R-MCD(+0.2) plasmid (kind gift from Dr. Gregory Jedd) and cloned into XLone-GFP plasmid using Gibson assembly kit (NEB, E2611S). Two days after nucleofection ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Each of the synthesized DNA fragments was amplified by PCR and inserted at the 5’ of the GFP gene in the pPha-NR vector (NovoPro Bioscience) using NEBuilder HiFi DNA Assembly (New England BioLabs). Five μg of the pPha-NR constructs were introduced into P ...
-
bioRxiv - Molecular Biology 2020Quote: ... These constructs were synthesized as gBlocks (Integrated DNA Technologies) and PCR-amplified before transfection using Q5 polymerase (New England Biolabs, Cat No. M0491S). To generate the repair template ...
-
bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
-
bioRxiv - Genomics 2020Quote: ... Long dsRNAs for R1 and R2 of the Dg vATPase A gene and lacZ control gene (120 μg of each dsRNA) were incubated with 0.2 units/μL ShortCut® RNase III (New England BioLabs, Ipswich, MA, USA) for 3 h at 37°C to produce a heterogeneous mix of short (18-25 bp ...
-
bioRxiv - Molecular Biology 2020Quote: ... fraction #2 of all samples as well as a positive control of U1 snRNP were incubated with 1 μl of proteinase K (NEB, 800 units/ml) for 30 min at 37 °C and loaded onto a denaturing gel (8% acrylamide/bis-acrylamide ...
-
bioRxiv - Neuroscience 2023Quote: ... rTg4510 mouse brain sections were incubated in the absence (control) or presence (treated) of 10,000 U/mL λ phosphatase (New England Biolabs, cat. no. P0753) for 24 hours at RT ...
-
bioRxiv - Biochemistry 2019Quote: ... The construct coding for the deletion mutant (ΔNt-LRAT-GFP) was generated by site directed mutagenesis (New England Biolabs, Ipswich, MA). CHO-k1 and LX-2 cells were transiently transfected by Lipofectamine 2000 (Thermo Fisher ...
-
bioRxiv - Genetics 2020Quote: ... a plasmid expressing a C-terminal fragment of TDP-43 (aa: 175-414) as GFP fusion was generated here using Q5 site-directed mutagenesis system (NEB, USA) using the plasmid pRS426-GAL1p-TDP43-GFP (Addgene ...
-
bioRxiv - Biophysics 2020Quote: ... The activity of the biotinylated ribosomes was checked by synthesizing GFP emerald (GFPem) in vitro (PURExpress Δ ribosomes, NEB #E3313S, New England Biolabs) and measuring fluorescence of GFPem in a QM-7 spectrofluorometer (Photon Technology International ...
-
bioRxiv - Cell Biology 2021Quote: ... dephosphorylated pcDNA5/FRT/TO-GFP-P2A-mcherry vector (kind gift from Manu Hegde, LMB-Cambridge) using Gibson assembly® (New England Biolabs). The SQS fluorescent reporter GFP-P2A-mcherry-SQS has been reported previously13 ...
-
bioRxiv - Cell Biology 2019Quote: ... The GFP-Rab5C PCR product was then ligated into the digested pLXIN-I-NeoR using T4 DNA Ligase (New England Biolabs – M0202). The ligated plasmid was amplified in bacteria selected with ampicillin and verified using Sanger Sequencing ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB, R3101S) and AgeI (NEB ...
-
bioRxiv - Genetics 2021Quote: ... We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.
-
bioRxiv - Physiology 2023Quote: ... primers for separate glnA and gfp fragments were generated using the NEBuilder Assembly Tool (neb.com, New England Biolabs Inc., MA, USA). The fragments carry overlapping segments to one another and with the pUA139 vector ...
-
bioRxiv - Cell Biology 2023Quote: ... G3BP1-GFP (V11A, F112A, F33A, F33W, F15A, F15W, F124A, F124W) were created by the Q5 Site-Directed Mutagenesis kit (NEB; E0554S).
-
Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Briefly 2 µM of dsDNA guide was ligated into 10 ng of pSp-Cas9-(BB)-2A-GFP using 400 units T4 DNA ligase (NEB M0202) and incubated for 16 hrs at 16°C ...
-
Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Oligonucleotides were ligated into pSp-Cas9-(BB)-2A-GFP plasmid that had been linearised by digestion with BbsI-HF (NEB R3539) and gel purified (Bioline BIO-52060 ...
-
bioRxiv - Genomics 2023Quote: ... The Myf5-promoter sequence was added at NdeI and KpeI cutting sites and the CRE-GFP sequence was added at SpeI and XhoI restriction sites using a T4 DNA ligase (NEB #M0202L). Linker sequence and primers used for PCR amplification are listed in Table S3 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... mAmetrine was inserted in place of GFP in both pDawn and pDusk plasmids via PCR and Gibson assembly (NEB HiFi mix). Using the same method ...
-
bioRxiv - Cell Biology 2023Quote: ... We assembled the pLenti-3HA-Clic4-T2A-GFP vector with pLenti backbone and fragments using NEBuilder HiFi DNA Assembly Master Mix (NEB, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Repair template was generated by using oligos P7/P8 to amplify GFP sequence from plasmid pCas9/sgRNA/Bleo with Phusion® High-Fidelity DNA Polymerase (New England Biolabs). ME49Δku8021 was transfected with 100 μg of gRNA and 20 μg of repair template ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
-
bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
-
bioRxiv - Developmental Biology 2023Quote: ... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
-
bioRxiv - Cell Biology 2023Quote: ... was cloned in frame with GFP in the BglII site of pJT61 56 using Gibson Assembly Master Mix (New England Biolabs Inc.)
-
bioRxiv - Developmental Biology 2024Quote: ... the regulatory region of each gene was inserted into SmaI digested pUC19 together with GFP amplified from pPD95.75 using NEBuilder® HiFi DNA Assembly Master Mix (NEB - E2621). PCR products or Plasmids were micro-injected into CB4088 [him-5(e1490)] hermaphrodites with myo-2::mCherry from plasmid pCFJ90 (Addgene ...
-
bioRxiv - Systems Biology 2023Quote: ... To remove any remaining phosphorylation the GFP-Trap bound proteins were treated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
-
bioRxiv - Genetics 2019Quote: ... were introduced into the flank region of GFP expression cassette in p123 plasmid in HindIII and EcoRV site sequentially by Gibson assembly (New England Biolabs, Ipswich, USA). The resulting plasmid p123-19MM was then linearized by SspI and transformed into SG200 protoplast as described previously (Schulz et al ...
-
bioRxiv - Plant Biology 2021Quote: ... the mutant gfp1a and gfp1b were created by inserting a gblcoks containing frame shift mutation sequences at the 5’ end of GFP coding sequence of pGFPGUSPlus (Plasmid #64401 in addgene) using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs, Catalog #E5520S). Then ...
-
ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: To obtain pDONR221-APEX.Tango1, the GFP sequence in pT-SP-G-Tango1 (Liu et al., 2017) was removed through NheI (New England Biolabs, cat # R3131L) and SpeI (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... pBGC was constructed by joining the amplified pBGT backbone and the gfp terminator region using the Gibson Assembly Cloning Kit (New England BioLabs, MA, USA). Resulting reaction was transformed by heat shock into NEB 5-alpha Competent E ...
-
bioRxiv - Microbiology 2022Quote: ... The purified fragments and the pABC3-His or pABC3-GFP vector were digested with PacI and NotI restriction enzymes (New England Biolabs, MA, USA). Resultant fragments were then gel-purified using the Monarch DNA Gel extraction kit (New England Biolabs ...
-
bioRxiv - Molecular Biology 2023Quote: ... This sequence was inserted into a modified pST50 vector56 containing N-terminal Strep-6xHis-TEV-GFP-PreScission tags using Gibson assembly (NEB Cat #: E2611L) to obtain a tagged construct of full-length HURP ...
-
bioRxiv - Cell Biology 2024Quote: ... These fragments were subcloned into pLenti-MCS-GFP-SV40-Puro empty vector that linearized by XbaI and SpeI restriction enzymes (New England Biolabs, Inc., USA) via Gibson Assembly kit (TransGen Biotech. ...
-
bioRxiv - Developmental Biology 2020Quote: ... In a first step the gfap promoter driving GFP was sub-cloned into a vector containing IsceI sites using NotI and XhoI restriction enzymes (NEB #R3189 and #R0146). To insert lssmKate2 SalI and NotI restriction enzymes (NEB #R3138S and #R3189 ...
-
bioRxiv - Biophysics 2020Quote: ... The activity of the biotinylated ribosomes was checked by synthesizing GFP emerald (GFPem) in vitro (PURExpress Δ ribosomes, NEB #E3313S, New England Biolabs) and measuring fluorescence of GFPem in a QM-7 spectrofluorometer (Photon Technology International ...
-
bioRxiv - Cell Biology 2021Quote: pcDNA4/TO-GFP-CLC7-Y715C mutation was generated by site-directed mutagenesis of the pcDNA4/TO-GFP-CLC7 expression vector using the Q5™ Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) and complementary primer pairs (forward ...
-
bioRxiv - Cancer Biology 2023Quote: ... dual guide gene blocks were ordered from IDT and cloned into the Lenti-Cas9-gRNA-GFP vector using NEBuilder HiFi DNA Assembly (New England Biolabs, cat. no. E2621L). CRISPR gRNA sequences are listed in Table S5A ...
-
bioRxiv - Genomics 2021Quote: SAMOSA was performed on remodeled arrays as well as unremodeled arrays and unassembled DNA controls using the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4 U/mL) as previously described (Abdulhay et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... from 4 to 15 hours at 37 °C. For Circle-Seq of the CRISPR treatments and controls (Fig. 5) exonuclease T (New England Biolabs; 20 units per μg of DNA) was then added ...
-
bioRxiv - Biophysics 2021Quote: A TetR-mCherry-antiGFPnanobody plasmid was constructed based on a pIRES-CMV-LS-dCas9-mCherry-antiGFPnanobody plasmid (Dahan lab) and a TetR-GFP plasmid (Dahan lab) through amplifications by Q5® High-Fidelity DNA polymerase (NEB, Cat. No M0492S) (primers ...