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Citations for New England Biolabs :
101 - 150 of 347 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-hRab22(S19N) (#1068) was generated using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions with forward primer #1556 (TGTAGGTAAAaacAGTATTGTGTGGCGG ...
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bioRxiv - Microbiology 2021Quote: ... The pGEMT amyE plasmid and the cat-gfp amplicon were both digested by KasI (NEB) and the two linear fragments with compatible 5’ overhangs were ligated together to obtain the PGEMT amyEup-cat-gfp-amyEdw plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... pLib vector was then ligated with the GFP insert using T7 DNA Ligase (NEB #M0318S), incubating at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... The vector backbone was amplified from pFA6a-GFP-KanMX6 using Phusion polymerase (New England BioLabs). Both PCR reactions were digested with DpnI and purified using an E.Z.N.A Cycle Pure Kit (Omega Bio-tek) ...
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bioRxiv - Cancer Biology 2022Quote: ... DoxON-ETV6(R399C)-V5-GFP was generated with the Q5 site-directed mutagenesis kit (NEB) per manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... GFP and Lphn2 were inserted into LV-UbC with Gibson assembly cloning kit (NEB E5510S). The Lphn2-F831A/M835A and Lphn2-T829G mutations were made using Q5 mutagenesis (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... We cloned these fragments into the CAG-GFP backbone by Gibson Assembly (NEB, Cat.No. E2611). Primers are listed in Table S1&2.
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bioRxiv - Biophysics 2023Quote: ... We made LV-PGK-GFP-KRAS(WT/G12V) by Gibson assembly (New England Biolabs, MA) that assembled three parts containing overlapping DNA ends into a 5.7kb lentiviral backbone.
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bioRxiv - Cell Biology 2023Quote: ... The resulting GFP construct was digested with KpnI and ApaI restriction enzymes (New England Biolabs), and ligated into an ApaI- and KpnI-digested pCDNA4:TO vector (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pMIG-GFP plasmid by restriction digestion method using Bgl2 and EcoR1 (NEB). Primers used for cloning (Forward primer ...
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bioRxiv - Plant Biology 2023Quote: ... Then we fused GFP to the C terminus of GUS through seamless cloning (NEBuilder, NEB) using primers KT1101 ...
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bioRxiv - Cell Biology 2023Quote: ... The gfp sequence was cloned and incorporated using NEBuilder HiFi DNA Assembly Master Mix (NEB). The col1a2-gfp sequence from the final pGEMT-col1a2-gfp construct was excised using restriction enzymes and inserted into the pTol2 vector ...
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bioRxiv - Neuroscience 2024Quote: ... to replace the GFP sequence using NEBuilder HiFi DNA assembly master mix (New England Biolabs).
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bioRxiv - Plant Biology 2024Quote: ... Mutagenesis of NGR1p::NGR1-GFP was performed using Gibson assembly (NEB HiFi DNA assembly E2621L), overlapping compatible cohesive ends were modified ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... A positive control GTase reaction using 0.5 U / μL of the vaccinia capping enzyme (New England Biolabs #M2080S) in place of the SARS-CoV-2 RdRp was performed under similar conditions ...
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bioRxiv - Biochemistry 2021Quote: ... Oligos (IDT) were cloned into lentiCRISPR-v1 (controls) or lentiCRISPR-v2 (SLC25A40 and TXNRD2) using T4 ligase (NEB). Ligation products were cloned into Escherichia coli (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Cell Biology 2023Quote: ... For an R-loop negative control the preparation was treated with 5 U RNaseH (M0297S, NEB Japan, Japan) in 1× RNaseH Reaction Buffer (NEB Japan ...
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bioRxiv - Microbiology 2023Quote: ... Hep3B cells and HBV control HepG2 2.2.15 cells and subjected to PCR amplification using the LongAmp Taq kit (NEB) and HBV primers spanning the CTCF binding motifs (HBV2 forward and Hep23B reverse) ...
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bioRxiv - Microbiology 2023Quote: ... These constructs were placed under the control of the T7 promoter and inserted into EcoRI (New England Biolabs) and NotI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Microbiology 2020Quote: ... The GFP fragment could then be replaced through restriction enzyme ligation with AscI (New England Biolabs) at the 5’ end and NotI (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... or GFP plasmid respectively were digested with XhoI for 1hr (New England Biolabs, Ipswich, MA, USA) for linearization ...
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bioRxiv - Cell Biology 2019Quote: The GFP nonstop and stop reporters were constructed by Gibson cloning (Gibson Assembly Master Mix, NEB) eGFP with or without a terminal stop codon into the pCMV AAV backbone which was used as an expression vector and used to make AAV ...
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bioRxiv - Genomics 2019Quote: ... We inserted the minimal promoter and GFP using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) as described above.
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bioRxiv - Evolutionary Biology 2020Quote: ... The resulting fragment encoding ZEB2-GFP-Flag was then digested with restriction enzymes AgeI (NEB, R3552S) and KpnI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... After overnight incubation at 4°C in PBS-Perm with rabbit anti-GFP (1:1000, Biolabs) and mouse anti-MAP2 (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... The Xlone-GFP backbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L) ...
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bioRxiv - Biophysics 2023Quote: ... The C2AW52R and C2AV67D mutations were introduced into GFP-dysferlin using Q5 mutagenesis (New England Biolabs).
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bioRxiv - Bioengineering 2023Quote: ... The final CBM-GFP constructs were assembled using NEBuilder® Hifi DNA Assembly (New England Biolabs) master mix following manufacturers protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA from the polyclonal parasites that returned from transfection was digested with BamHI and SpeI (New England Biolabs) and transferred to membrane (Nytran SuPerCharge ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... and No Template Controls (NTC) for each primer pair using Luna Universal qCPR Master Mix (NEB, Ipswich, MA, #M3003) following the manufacturer’s protocol (1ul cDNA ...
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bioRxiv - Genomics 2021Quote: The qPCR reactions were performed with enzymatic selection or control samples using Luna Universal qPCR Master Mix (NEB #M3003S) on a Bio-Rad CFX96 real-time PCR detection system ...
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bioRxiv - Bioengineering 2021Quote: ... Taq and OneTaq® Hot Start DNA polymerases as well as the control nicking enzymes Nt.BstNBI were purchased from New England Biolabs (NEB). Details on sample preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole-genome sequencing and library preparations for tumors and matched germline controls were performed according to the manufacturer’s instructions (Illumina, San Diego, CA, USA or NEBNext, NEB). The quality of the libraries was assessed using a Bioanalyzer (Agilent ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the pre-cleared lysates were collected and mixed with the biotin labeled pre-miRNA or biotin labeled shuffled pre-miRNA as negative control in the final concentration of 8.23 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Neuroscience 2021Quote: The expression clone for both the peptide of interest and the control was transformed into competent DH5α cells (NEB® [New England Biolabs] 5-alpha Competent E ...
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bioRxiv - Plant Biology 2022Quote: ... 2.5 μl of A-Tailing control and 12.5 μl of A-Tailing mixture (New England Biolabs, Ipswich, MA, USA) was added to the DNA solution in a final volume of 30 μl and vortexed at 1,800 × g for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... GRCh37/hg19) spanning the APA control region in DNAJB6 were generated using PCR (EpiMark-HS-TAQ, New England Biolabs), where one set of reactions used a 5-methylcytosine-containing dNTP mix (methylated probes ...
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bioRxiv - Microbiology 2023Quote: ... Non-β-lactamase genes used as controls were cloned into the pBAD18 using the Gibson Assembly Cloning Kit (NEB). pBAD (RBS)_fwd/rev primer pairs were used to amplify the plasmid backbone ...
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bioRxiv - Molecular Biology 2024Quote: ... The crosslink bearing oligo and an uncrosslinked control was then phosphorylated by T4 polynucleotide kinase (New England Biolabs M0201S).
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bioRxiv - Cell Biology 2022Quote: ... The beads to which GFP-DSB-1 had been immobilized were subject to the phosphatase assay (NEB lambda phosphatase #P0753S ...
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2019Quote: ... verticillioides with the primers FvGbb2-GFP-F/R using Q5® High-Fidelity DNA Polymerase (NEB: M0491S). The DNA fragment was introduced to the pKNTG plasmid KpnI and HindIII sites via In-Fusion-HD cloning kit (Takara Bio USA ...