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Citations for New England Biolabs :
1 - 50 of 347 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: Plasmids carrying the gfp reporter under the control of different versions of hisJ promoter were constructed using the NEBuilder HiFi DNA Assembly (NEB). Primers used to amplify the backbone or the promoter regions from the evolved bacterial lines are reported in the primer list ...
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bioRxiv - Cell Biology 2021Quote: ... and the GFP-KLP-18ts and GFP-ΔC-stalk were created via site-directed mutagenesis (NEB) from this GFP-stalk construct ...
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bioRxiv - Molecular Biology 2023Quote: The linear ssDNA scaffold for our designs as the positive control and negative control was obtained by cutting circular M13mp18 DNA (New England Biolabs) at BamHI-HF and EcoRI-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Before transfection p70intsce was linearized with Not1 (NEB).
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bioRxiv - Cell Biology 2020Quote: ... Constructs were linearized for transfection with NotI (NEB) and transfected into the 29-13 cell line ...
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bioRxiv - Immunology 2023Quote: mHSP70 or fetuin (New England Biolabs, positive control) were subjected to cleavage by PNGase F (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... or amylose resin (negative control; New England Biolabs) at 4°C for 3 h ...
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bioRxiv - Developmental Biology 2020Quote: ... GFP-MyoXΔPH and GFP-MyoXΔFERM were generated with Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S), where the amino acids (1212-1253 ...
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bioRxiv - Cell Biology 2021Quote: All constructs (GFP-EXO70B2, EXO70B2-GFP and mRuby2-EXO70B2) were prepared with the Phusion PC (NEB, USA) reaction using as a template either genomic DNA for intron-less genes and promoters or cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... and iLID constructs were generated by PCR amplifying the parent constructs in pcDNA3.1 (GFP-Gγ3, GFP-Gγ9, and Venus-iLID) with overhangs containing expected nucleotide mutations and DpnI (NEB) digestion (to remove the parent construct ...
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bioRxiv - Cell Biology 2020Quote: ... and pFLOE1p:FLOE1ΔDUF-GFP were obtained by modifying pFLOE1p:FLOE1-GFP using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priDSdeletion-FWD/REV ...
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bioRxiv - Neuroscience 2023Quote: ... We prepared cDNA libraries from GFP+ and GFP-samples (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB) from RNA enriched with mRNA through bead-based polyA selection ...
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bioRxiv - Immunology 2020Quote: ... A positive control on the other hand was established by transforming DH5α bacteria with NEBuilder® Positive Control (New England Biolabs, USA). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... m6A-modified RNA spike-in controls (New England Biolabs) and a nontargeted region on the crRNA-targeted transcript ...
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bioRxiv - Cell Biology 2019Quote: ... Untreated controls were supplemented with GlycoBuffer3 (New England Biolabs). Reactions were stopped by treatment with SDS-PAGE sample buffer containing benzonase nuclease (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... As a control Escherichia coli ribosomes (P0763S, NEB, Australia) were used in 4 μL aliquots (approx ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: A deletion between amino acid 336 and the beginning of GFP was generated from the MCSV:GPR68-GFP plasmid using the Q5 mutagenesis kit (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... Barhl1a riboprobe was synthesized as previously reporter 19 and gfp riboprobe was synthesized from a linearized pCS2:GFP plasmid with NotI (Fermentas or New England Biolabs) and transcribed with Sp6 (mMessage mMachine Sp6 ...
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bioRxiv - Cell Biology 2021Quote: ... The β1 Integrin-HaloTag-GFP without ARL13B–C terminus (I-GFP) was generated by Q5 site directed mutagenesis (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... σ3 WT and mutant sequences flanked by BamHI and EcoRI sites were synthesized commercially by ATUM and then cloned into pWPXL-GFP plasmids to replace GFP sequence through T4 ligation (NEB). pWPXL-Flag and pWPXL-HA vectors were generated by ATUM by replacing GFP with Flag- or HA-‘stuffer sequences’ flanked by BamHI and EcoRI sites ...
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bioRxiv - Cell Biology 2023Quote: ... pCIG2-SNAP-M87 (generated for this study from pCIG2-GFP-MACF43 by replacing GFP with SNAP-tag from pSNAPf vector (New England Biolabs) and MACF43 with M87 from pCMV-Tag/WT M87) ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: To generate a BAC vector containing UAS-SPA-GFP a Drosophila codon optimized SPA-GFP was synthesized as a gBlock (IDT) and cloned by Gibson assembly (NEB) into pIM147 in place of GCaMP6 to generated pIM141.
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bioRxiv - Cell Biology 2024Quote: ... Before transfection pKD plasmids were digested overnight with EcoRV (NEB). The enzyme was then subjected to heat inactivation for 20 min at 65 °C and then mixed with the pUF1-Cas9 plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... or positive controls doxorubicin (50 μM; New England Biolabs 5927) and antimycin A (10 μM ...
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bioRxiv - Immunology 2019Quote: ... pSV40-CLuc control plasmid was obtained from NEB (Ipswich, MA). These constructs were co-transfected to 293 cells by FugeneHD (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... Controls were handled with same protocol without λ-phosphatase (NEB). Phospho-mass spectrometry was performed as previously described (Nagai et al. ...
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bioRxiv - Microbiology 2020Quote: ... Endo H controls: Cells were treated with Endo H (NEB, 1:10 in glycobuffer 3 ...
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bioRxiv - Cell Biology 2021Quote: ... The m6A-modified and unmodified control RNAs (New England Biolabs) were divided into fragmented RNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Cancer Biology 2023Quote: ... A negative control treated overnight with RNaseH1 (New England Biolabs) was included ...
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bioRxiv - Cell Biology 2022Quote: pLV-PKR-GFP was generated from pPKR-GFP and pLV-EF1-Cre-PGK-Puro using NEBuilder® HiFi DNA Assembly (NEB #E5520) and the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... mutant (GFP-FIP3-I738E-ABD) were engineered from the McCaffrey plasmids GFP-FIP3 and GFP-FIP3I738E using the Q5 Mutagenesis Kit (New England Biolabs, Ipswich, MA) following manufacturer instructions ...
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bioRxiv - Systems Biology 2022Quote: pAK-Tol2-TRE-JunDN-HA-T2A-GFP (pTRE-JunDN-HA-T2A-GFP) construct was assembled using Gibson assembly with NEBuilder HiFi DNA Assembly Master Mix (NEB, catalog # E2621) and inserted into a lentiviral pLVX-CMV empty backbone ...
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bioRxiv - Microbiology 2022Quote: ... and replaced it with the sequence encoding GFP in the pT7-RRV-NSP3-GFP constructed previously (12) by NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, cat. #E2621S). To construct expression vectors encoding HuNoV-VP1 (pCAG-HuNoV-VP1 ...
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bioRxiv - Biochemistry 2019Quote: ... into C1-GFP using the restriction sites NotI (NEB) and BsrGI (NEB ...
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bioRxiv - Genomics 2020Quote: Transfection of gRNA and Cas9 ribonucleoprotein (EnGen SpyCas9, New England Biolabs) into mESCs was performed using Neon Transfection System ...
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bioRxiv - Cell Biology 2020Quote: ... Renilla luciferase was then ligated into pGL3-Control digested HindIII (NEB) to make pGL3-Renilla-FF ...
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bioRxiv - Developmental Biology 2021Quote: 4mC and 5mC spike-in controls were generated from pUC19 (NEB) PCR product synthesized with N4-methyl-dCTP (4mdCTP ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Immunology 2021Quote: ... or an appropriate isotype-matched control (polyclonal goat isotype IgG, NEB) was added at a final concentration of 10 μg/ml and incubated overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested 30 hours after transfection using Passive Lysis Buffer (NEB) and luciferase activity determined with Bright-Glo luciferin reagent (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... and LV-H2B-GFP backbone was also dephosphorylated (CIP, NEB). Resulting DNA fragments were directly column purified (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... GFP was replaced with mRuby using BamHI (New England Biolabs) and XbaI restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... pLenti-Cas9-GFP was digested with BsmBI (New England Biolabs). Primers listed in supplemental table 1 were annealed and ligated into the digested plasmid using T4 ligase (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... pLenti-GFP was digested by restriction enzymes AsiSI (NEB, R0630S) and MluI-HF (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Cell Biology 2021Quote: ... A positive control was prepared by substituting casein kinase II(NEB, P6010S) for galectin 3 in the reaction mixture.