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Citations for New England Biolabs :
3001 - 3050 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Poly(A) mRNA was prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs) and 2 × 100bp paired-end sequencing at a depth of 80-100 million reads per sample was performed using the Illumina Novaseq 6000 platform by the University of Toronto Donelly Sequencing Centre ...
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bioRxiv - Molecular Biology 2021Quote: ... The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7765). The final RNA-seq libraries were amplified with 7 – 8 PCR cycles.
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bioRxiv - Genomics 2022Quote: ... and then ‘A’ base was added to 3’ blunt ends using the A-Tailing reaction (NEBNext® UltraTM II End Repair/dA-Tailing Module, NEB, Cat. E7546). The purified A-tailed DNA was ligated with adaptors from the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Genomics 2020Quote: ... 75 ng for 37PF and 100 ng for 362YU3) were fragmented using the NEBNext® Ultra™ II FS DNA kit (New England Biolabs, USA) at 37°C for 20 minutes (resulting in DNA fragments with an average size of 350 bp) ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared from total RNA using NEBNext® Ultra™ II RNA Library Prep for Illumina (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... plasmids that contained the DNA fragments I and II were digested using respectively Agel/Fsel and Smal/Dral restriction enzymes (New England BioLabs, Ipswich, MA, USA) (Figure 4) ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was done using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to manufacturer instructions with 4 cycles of PCR amplification ...
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bioRxiv - Genomics 2019Quote: ... Glucosylated DNA was then end repaired without purification followed by ligation to Pyrrolo-dC adaptors as indicated in NEBNext Ultra II DNA library preparation protocol (NEB, Ipswich, MA, USA) with the following modification to the protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to evaporate the samples to enable miniaturized mNGS library preparation with the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA). Library preparation was performed per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Libraries from purified CD14+ monocytes RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA), which allows for lower input concentrations of RNA (10ng) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was used to prepare 48 separate cDNA libraries to produce 100 base pair paired end reads using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA). Each library was individually barcoded with NEBNext dual adaptors (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were concentrated and purified with a Monarch® PCR & DNA Cleanup Kit and the entire 8 μl of eluted product was used as a substrate for Illumina sequencing library construction using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) and the protocol provided with the kit ...
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bioRxiv - Systems Biology 2021Quote: RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from a high-titer ϕHNL01 lysate and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs, Ipswich, MA, USA). Libraries were quantified using a BioAnalyzer (Agilent ...
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bioRxiv - Plant Biology 2021Quote: ... all RNA-seq libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (catalog number E7765; New England Biolabs) using 500 ng of total RNA as starting material ...
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bioRxiv - Biochemistry 2020Quote: ... and used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), following manufacturer instructions.
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bioRxiv - Biochemistry 2020Quote: ... Reverse transcription reactions were then used as input for the NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, cat. E6111L). Second strand synthesis was performed by incubating 1 h at 16°C ...
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bioRxiv - Microbiology 2020Quote: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 50ng of the amplicons were taken for end prep with NEBNext ULTRA II End Repair/dA tailing module (Cat. No. E7546, New England Biolabs, Ipswich, Massachusetts, USA) followed by incubation at 20°C for 5 mins and 65°C for 5mins ...
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bioRxiv - Microbiology 2021Quote: ... The resulting sheared DNA was used for library preparation using the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The amplified mRNA of each sample was used to construct sequencing libraries using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, USA) according to the instruction manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed to enrich for sub-nucleosomal fragments using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina as described (NEB, E7645S). The libraries were size-selected and purified with AMPure XP beads ...
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bioRxiv - Developmental Biology 2022Quote: ... and 500 ng of RNA was used for cDNA library construction with NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). cDNA libraries were validated on the TapeStation (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Five microliters of resulting clean RNA was used for first- and second-strand cDNA synthesis and library preparation using NEB Ultra II Directional RNA library prep kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were barcoded with unique dual indices synthesized in the CDC Biotechnology Core Facility Oligonucleotide Synthesis Laboratory ...
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bioRxiv - Genomics 2022Quote: ... The extracted RNA was used for library preparation (NEBNext Ultra II RNA Library Prep with Sample Purification Beads, #E7775S) with appropriate barcoded sequencing primers (NEB #E7335-12 indices).
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bioRxiv - Genetics 2022Quote: ... and end-repair processing were performed with the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). The cDNA libraries were adapter-ligated using adapters and unique dual indexes in the NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and further ligated with illumina adaptor sequences using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). The prepared libraries were purified using AMPure XP beads and sequenced as 2 × 250 bp on MiSeq Reagent Kit v2 (illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-C library was prepared on beads using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext® Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima Hi-C kit ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (for old herbarium specimens) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (for more recent specimens; New England BioLabs, Ipswich, USA). In both cases ...
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bioRxiv - Cancer Biology 2019Quote: ... Libraries for whole genome sequencing were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Inc., Ipswich, MA). Libraries were sequenced on a NovaSeq S1 flow cell (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the eluted RNA was input to the library prep using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (E7765S, New England Biolabs, Ipswich, MA, USA), NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic localization of BioTAP-Pcl and -Jarid was determined by high-throughput DNA sequencing of libraries generated from BioTAP-XL purified genomic DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7645) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The sheared DNA was prepared for sequencing library preparation with the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Strand-specific cDNA libraries were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Sequencing was performed using NovaSeq6000 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of RNA was used to create indexed cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs, Ipswich, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA) per manufacturer recommendations with custom inline barcodes as described in Moore et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England BioLabs® Inc., USA). For sequencing ...
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bioRxiv - Microbiology 2019Quote: ... coli DNA and 2 μg of Salmonella DNA in a total of 100 μL each were added to the NEBNext® Ultra™ II End Repair/dA-Tailing module (New England Biolabs (NEB), USA ...
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bioRxiv - Genomics 2019Quote: ... Short-insert of 250 bp paired-end libraries were prepared for both individuals using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Biolabs) and 10 PCR cycles ...
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bioRxiv - Cell Biology 2019Quote: ... and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Physiology 2019Quote: ... The synthesis of cDNA for reverse transcription was carried out with a Protoscript II first strand cDNA synthesis kit (New England Biolabs, Ipswich, MA, USA) using 120 ng total RNA and 60 μM random hexamers ...
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bioRxiv - Physiology 2020Quote: ... Sequencing library was prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Cat # E7770S) following manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Library preparation for sequencing was performed using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645, New England Biolabs NEB). Samples were sequenced paired-end ...
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bioRxiv - Evolutionary Biology 2020Quote: ... dual-indexed Illumina genomic libraries were prepared for each DNA sample using an insert size of ~350 bp and the Ultra II Library Prep Kit (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2013) with the exception of the end-repair step using NEBNext® Ultra II End Repair/dA-Tailing module (NEB, cat. no. E7546L) accordingly to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA libraries were prepared in a paired-end layout with NEBNext® Ultra™ II FS DNA Library Prep Kit (New England BioLabs, UK) and sequenced under a 150-bp read length using Illumina HiSeq 4000 systems and HiSeq SBS Kit v4 reagents (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNAs were synthesised from 1 μg of total RNA in a 20 μL reaction volume using 200 U of Protoscript II reverse transcriptase (#M0368S; NEB, Ipswich, MA, USA), oligo (dT17 ...