Labshake search
Citations for New England Biolabs :
3201 - 3250 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs, Ipswich, MA, USA) and sequenced on two lanes (two chips ...
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bioRxiv - Systems Biology 2021Quote: ... The remaining mRNA was used for stranded RNA-seq library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genomics 2021Quote: ... were repaired and 3’-adenylated with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA). Sequencing adapters provided by Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd ...
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bioRxiv - Genomics 2021Quote: ... The selected DNA fragments were end-repaired and 3’-adenylated with the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA). The DNA was then purified with AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Genetics 2020Quote: ... 5 ng of fragmented DNA were then used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), as per manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... mRNA Magnetic Isolation Module in combination with the NEBNext® Ultra™ II Directional RNA Library Prep Kit and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Fragmentation was carried out using incubation conditions recommended by the manufacturer for samples with a RIN ≥7 to produce RNA sizes ≥ 300bp (94°C for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... cDNA was subjected to DNA repair and end-prep using the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents (NEB, catalog no. M6630 and E7546) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Second strand was synthesized by using the NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... For the library preparation of the pools the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich (MA) – United States of America ...
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bioRxiv - Neuroscience 2023Quote: ... Both input samples without IP and m1A input samples were used for library construction using an NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., MA, USA). Eluted RNA fragments were converted to cDNA and sequenced or treated to induce partial m1A to m6A conversion before cDNA synthesis ...
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bioRxiv - Neuroscience 2022Quote: ... Polyadenylated RNA was captured from 50 ng of total RNA per sample and libraries were prepared using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Paired-end sequencing was done at the Vanderbilt VANTAGE core laboratory (Vanderbilt University ...
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bioRxiv - Genomics 2022Quote: ... then repaired and 3’-adenylated with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA). Sequencing adapters provided by Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated mRNA material was used to prepare the libraries using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs, Ipswich, MA, USA). Each library was individually labeled and subjected to 12 PCR cycles for final enrichment and pooled in equal quantities ...
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bioRxiv - Genomics 2024Quote: ... 400 ng DNA was aliquoted per barcoded sample for end and single-strand nick repair using NEBNext Ultra II End repair/dA-tailing Module and NEBNext FFPE Repair Mix (New England Biolabs UK, Catalogue numbers: M6630S, E7546S), with samples then being cleaned using AMPure XP Beads (Beckman Coulter UK ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Immunology 2022Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Immunology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Paired-end libraries of 2 x 150 bp fragments with an insert size of 330 bp were prepared with NEBNext® Ultra™ II DNA Library Prep Kits (New England Biolabs, Ipswich, MA, USA), and sequencing was performed on a HiSeq4000 Illumina sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mRNA material was used to prepare the libraries with the use of NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1 µg of the resulting DNA was used for library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB cat. E7103). Library PCRs were supplemented with 1× EvaGreen (Jena Bioscience cat ...
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bioRxiv - Immunology 2023Quote: ... dual unique indexed libraries were made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Manufacturer protocol was modified by diluting adapter 1:30 and using 3μL of this dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Sequencing of RNA samples was performed by Azenta Life Sciences (Burlington, Massachusetts, US) with NEBNext®Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, Massachusetts, US) for library preparation.
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bioRxiv - Genomics 2023Quote: ... BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Supplementary Table S7) in the following reaction mix: 10 µl 2X NEBNext® Ultra™ II Q5® Master Mix (NEB cat. M0544S), 1 µl 10 µM each bio-primer ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation for gene expression analysis was performed with the NEBNext® Poly(A) mRNA magnetic isolation module and NEBNext® UltraTM II Directional mRNA kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions with 1μg total RNA as input material ...
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bioRxiv - Molecular Biology 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: All adapter-ligated amplicons were amplified with custom iTru5 and iTru7 index primers (Glenn et al. 2019) with NEBNext Ultra II Q5 Master Mix (NEB, Cat No, M0544, Ipswich, MA) according to reaction conditions described in Supplemental Table 6 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries for good quality samples (total DNA amount > 20 ng) were prepared with the standard NEBNext®Ultra™ II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA), while double stranded libraries for the samples extracted in the ancient DNA laboratory were prepared following the Meyer and Kircher protocol67 ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed with TBS-T and then incubated with an anti-rabbit horseradish peroxidase conjugated secondary antibody (New England Biolabs, 1:10,000 in 5% skim milk in TBS-T) for 2h ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
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bioRxiv - Molecular Biology 2020Quote: 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat # E7760L; New England Biolabs, Ipswich, USA; protocol v. v3.1_5/20) including PolyA selection ...
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bioRxiv - Plant Biology 2022Quote: ... Paired end libraries for Illumina sequencing were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Inc, MA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... All libraries were constructed according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). The libraries were quantified by Qubit 2.0 and their quality was assessed by TapeStation D1000 ScreenTape (Agilent Technologies Inc. ...
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bioRxiv - Microbiology 2020Quote: Libraries were prepared for RNA sequencing according to the instructions of the NEBNextl® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs, Ipswich, MA, USA), with the following exception ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA; input 60 ng). Manufacturer’s instructions were followed except for Step 4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Libraries were prepared using 250 ng of isolated genomic DNA following the NEBNext® Ultra™ II FS DNA Library Prep (New England Biolabs, Frankfurt am Main, Germany) with Sample Purification Beads (NEB E6177S) ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA library preparation was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (# E7645S/L, New England BioLabs, Frankfurt am Main, Germany) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: DNA fragments recovered from S1 nuclease mapping and ChIP experiments were used to construct Illumina sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog No. E7645L) NEBNext® Multiplex Oligos for Illumina® Index Primer sets 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 100 ng RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (#57760S and #57765S, New England Biolabs, Frankfurt am Main, Germany) with the NEBNext® Poly(A ...
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bioRxiv - Microbiology 2023Quote: ... we prepped samples with the NEBNext® FFPE DNA Repair Mix and NEB-Next® Ultra™ II End Repair/dA-Tailing kits (New England Biolabs, Ipswich, MA, USA) and performed DNA cleanup Ampure XP beads (Beckman Coulter ...