Labshake search
Citations for New England Biolabs :
3101 - 3150 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries were generated with about 330ng DNA and the NEBNext Ultra II FS DNA Library Prep Kit in combination with NEBNext Multiplex Oligos (New England Biolabs, Ipswich, MA, USA). We sequenced 2×125bp reads with an Illumina HiSeq 2500 at the VBCF-NGS facility (https://www.viennabiocenter.org/vbcf/next-generation-sequencing/) ...
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bioRxiv - Microbiology 2023Quote: ... using the NEBNext Ultra II Directional RNA Library Prep Kit and the NEBNext Multiplex Oligos for Illumina (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and end-repair processing was performed using the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). We ligated adapters in the cDNA library using adapters and unique dual indexes from the NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... Libraries from purified progenitor RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). rRNA depleted RNA was fragmented ...
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bioRxiv - Genomics 2023Quote: ... Libraries of the fragmented DNA were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified for high-throughput sequencing by an Ultra II DNA Library Prep Kit (New England Biolabs, Catalog Number: E7645S). Then ...
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bioRxiv - Genomics 2023Quote: ... The other library was shipped frozen to the University of Oregon (Eugene, OR) and prepared for Illumina Novaseq sequencing using the NEB Ultra II kit (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with a few modifications by the Genomics and Cell Characterization Facility (GC3F ...
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bioRxiv - Genomics 2023Quote: ... a standard whole-genome 150 base pair (bp) paired-end Illumina library was prepared using the NEBNext Ultra II library preparation kit (New England Biolabs Inc., Ipswich, USA). Finally ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Developmental Biology 2023Quote: ... and the enriched mRNA was prepped for sequencing with the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB, #E7765S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... At day 4 post nucleofection genomic DNA was extracted and 500 ng of gDNA from each sample were randomly digested with NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E6177) to obtain fragments of ca ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA pellet was resuspended in 0.1 X TE and used for DNA library generation with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit) ...
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bioRxiv - Genetics 2023Quote: ... RNA libraries for RNA-seq were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760L) following manufacturer’s protocol for use with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was fragmented and TruSeq-Adapters ligated with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® (NEB) and 3’-end-fragments were finally amplified using primers with Illumina P5 and P7 overhangs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Some libraries have been instead prepared with the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Samples were either double indexed with a set of 60 dual-index primers ...
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bioRxiv - Cancer Biology 2023Quote: ... and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760). The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... to pull down mRNA and used for cDNA library construction using NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). The quality of the cDNA libraries were assessed on the TapeStation (Agilent ...
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bioRxiv - Genomics 2024Quote: ... Amplicons prepared for long-Illumina read sequencing (PE300) underwent end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA). Custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix with a 15 min incubation at 20°C (Supplemental Table 6) ...
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bioRxiv - Microbiology 2024Quote: ... a 20uL 16S-V4 PCR reaction was set up (1ng extracted gDNA; 1uL forward barcoded P5 primer; 1uL reverse barcoded P7 primer; 10uL NEBNext® Ultra™ II Q5® Master Mix [NEB M0544X] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... USA) or the University of Exeter sequencing service (Exeter, UK) following the NEBnext Ultra II FS protocol (New England Biolabs, Ipswich, MA, USA). Targeted bait capture was performed with a subfamily-specific ‘Mimobaits’ bait set (Nicholls et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ChIP DNA and 220 ng of whole cell extract DNA were used to prepare ChIP-seq libraries using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645S) with PCR amplifications carried out for 16 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently used to assemble the RNA-seq library using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB; E7760), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated DNA was washed once with 70% ethanol and suspended in 30μl 0.1 X TE (1mM Tris-HCl and 0.1mM EDTA) for library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 5 ng of the resulting RNA was applied for library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A library was then prepared following the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit procedure (New England Biolabs, Ipswich, MA, USA). A library was directly prepared from 100 ng of total RNA extracted from floral buds using the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit ...
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bioRxiv - Genetics 2024Quote: To determine the T-DNA insertion site of line ClvRubq7 we extracted genomic DNA from two different plants and constructed sequencing libraries using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E7805) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound antibodies were detected by incubation with anti-rabbit or anti-mouse DyLight 800-conjugated secondary antibodies (New England BioLabs). Slide images were acquired using an InnoScan 710-IR scanner (Innopsys ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
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bioRxiv - Immunology 2022Quote: The antibody expression vectors for each recombinant antibody (VH + VL-L/k) were transfected together with a Transposase vector (Hera BioLabs, USA). Cells were selected with Hygromycin B (H3274 ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were constructed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina according to manufacturer’s instructions (NEB - catalogue no. E7645S). Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no ...
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bioRxiv - Microbiology 2021Quote: ... The RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was determined using a Nanodrop and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Molecular Biology 2021Quote: ... following the manufacturer’s instructions and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Evolutionary Biology 2020Quote: Sequencing libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (E7645) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (E7805) (New England BioLabs, Ipswich, USA). Sequencing was conducted on an Illumina MiSeq System (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: Extracted and purified RNA was reverse transcribed into first-strand copy DNA (cDNA) using the ProtoScript® II Reverse Transcriptase Kit (NEW ENGLAND BioLabs®Inc, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The described method follows the NEBNext® Ultra™ II DNA Library Prep Kit (v4.0) with Sample Purification Beads Kit (New England Biolabs, Ipswich, Massachusetts, USA). But other kits can be used too ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was conducted using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: Whole Genome DNA libraries with an average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Genomics 2020Quote: ... Approximately 300 ng of genomic DNA was used to prepare DNA sequencing libraries for each parent following the protocol of NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB Cat No. #E7805S/L). The libraries were sent to Novogene (https://en.novogene.com/ ...
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bioRxiv - Microbiology 2021Quote: ... Repair and end-prep of DNA molecules was carried out using NEBNext FFPE DNA Repair and Ultra II End Repair/dA-Tailing modules (New England Biolabs Inc., Ipswich, United States), incubated at 20° C for 5 minutes followed by heat-inactivation at 65° C for an additional 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7760S, E7335). Library size (~350 bp ...
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bioRxiv - Plant Biology 2021Quote: The RNA-seq libraries were constructed with the NEB Next® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer’s instruction (NEB, Cat#E7775).
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bioRxiv - Microbiology 2019Quote: ... Metatranscriptomic libraries were prepared from total RNA using the NEBNext® Ultra II Directional RNA Sequencing Prep Kit (New England BioLabs, Ipswich, MA, USA) in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: Metagenomic libraries were prepared from DNA (100 ng) using the NEBNext® Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) > 100ng input protocol as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Genomics 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Cancer Biology 2022Quote: ... CUT&RUN barcoded libraries for Illumina sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) accordingly with Zhu et al.(Zhu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and phloem tissues were also prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs®) ...