Labshake search
Citations for New England Biolabs :
3151 - 3200 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat no. E7770, New England Biolabs, Ipswich MA). Single-end sequencing was performed using the Illumina NextSeq500 platform according to standard operating procedures.
-
bioRxiv - Molecular Biology 2019Quote: Libraries for next generation sequencing were prepared by using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645) following the protocol supplied with the kit and individual samples were indexed during the PCR step with NEBNext® Multiplex Oligos for Illumina® ...
-
bioRxiv - Genomics 2021Quote: Whole Genome DNA libraries with average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
-
bioRxiv - Genomics 2021Quote: ... cDNA libraries were synthesized using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Both: New England Biolabs, Ipswich, Massachusetts) and subsequently sequenced in a paired-end mode with 75bp read length on NextSeq500 (Illumina ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μg gDNA was then used to prepare the gDNA sample library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... The fragmented DNA was enzymatically repaired and end-modified with adenosine (NEBNext® Ultra™ II End Repair/dA-Tailing Module, NEB, Ipswich, MA) and ligated (NEBNext® Ultra ™ II Ligation Module ...
-
bioRxiv - Cell Biology 2022Quote: ... We created DNA libraries with an average library size of 320 bp using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... with primer-OCY581-NGS-Lib-KO-Fwd-first-step and OCY582-NGS-Lib-KO-Rev-first-step was performed using NEBNext® Ultra™ II Q5® Master Mix (New England Biolabs, M0544L). The first-step PCR products were recovered with QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Genomics 2022Quote: ... Library preparation and sequencing were prepared with Illumina-compatible NEBNext® Ultra™ II using Directional RNA Library Prep Kit (New England BioLabs, MA, USA). Viral total RNA was taken for fragmentation and priming ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... We used a standard miniaturized protocol to prepare DNA libraries using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA))56 ...
-
bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
-
bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared from the enriched mRNAs using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA). The library concentration was determined in a Qubit3 Fluorometer using the Qubit dsDNA HS (High Sensitivity ...
-
bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
-
bioRxiv - Plant Biology 2024Quote: Arabidopsis thaliana RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Preparation Kit (New England BioLabs, MA, USA). Five hundred ng of total RNA was taken for mRNA isolation ...
-
bioRxiv - Neuroscience 2024Quote: ... Stranded cDNA libraries were constructed using 200 ng of total RNA per sample and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... For Psav we used a standard miniaturized protocol to prepare DNA libraries for sequencing using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA)) ...
-
bioRxiv - Genetics 2024Quote: For library construction the ChIP purified DNA was used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645L) with modifications of the manufacturer instruction as we assembled half volume reactions ...
-
bioRxiv - Molecular Biology 2023Quote: ... These immuno-enriched DNA samples could be used for NGS after DNA library construction with NEBNext® UltraTM II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co. ...
-
bioRxiv - Cancer Biology 2022Quote: ... the fragments were purified and used for adapter ligation and library amplification using the NEBNext Ultra II Fs DNA Library kit for Illumina (NEB # E7805, New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
-
bioRxiv - Plant Biology 2023Quote: ... and then the sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan Inc., Tokyo, Japan). The library was sequenced using the illumina NovaSeq 6000 platform with S4 flow cells to produce 150 bp paired-end and dual index reads ...
-
bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (New England BioLabs, Frankfurt am Main, Germany). Sequencing was performed by using the HiSeq2500 instrument (Illumina Inc. ...
-
bioRxiv - Cancer Biology 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, Cat. No. E7490L, E7760L) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: Libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Individual genomic libraries for the NGS experiments were constructed with the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) with inputs ≤ 100 ng (Supplementary information) ...
-
bioRxiv - Microbiology 2023Quote: ... samples were repaired and end-prepped using the NEBNext® FFPE DNA Repair Mix and NEBNext® Ultra™ II End Repair/dA-Tailing kits (New England Biolabs, USA). DNA was cleaned using Ampure XP Beads (Beckman Coulter ...
-
bioRxiv - Developmental Biology 2023Quote: ... libraries using 500 ng of good-quality RNA (RIN ˃7and a NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA, #E7760L) at the Genome Québec core facility and paired-end sequenced on a NovaSeq6000 S4 sequencer ...
-
bioRxiv - Bioengineering 2023Quote: ... All 5μl of the cell lysis were used for each Genomic PCR amplification using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S). PCR program is 1 cycle ...
-
bioRxiv - Cancer Biology 2023Quote: ... Amplified DNA was fragmented and ligated to adapter using instruction and reagents provided by NEBNext® Ultra™ II FS DNA Library Prep (NEB, cat.no. E7805S). Finally ...
-
bioRxiv - Microbiology 2023Quote: ... the products were then used to construct sequencing libraries using the NEB Next® Ultra II™ DNA Library Prep Kit for Illumina® (New England Biolabs, USA) following the manufacturer’s recommendations.
-
bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S, New England Biolabs, Ipswich, MA). The isolated mRNA was fragmented ...
-
bioRxiv - Genomics 2023Quote: ... The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with Unique Dual Index UMI Adapters RNA Set1 (NEB, Franklin Lake, NJ, USA) were used in the succeeding steps according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... The RNA library preparation was performed following the NEB NEBNext Ultra II Directional RNA Library Preparation protocol (New England Biolabs Inc., Ipswich, MA, USA). RNA libraries were loaded on a Bioanalyzer 2100 DNA High Sensitivity chip to check for size ...
-
bioRxiv - Microbiology 2024Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Frankfurt am Main, Germany). To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies) ...
-
bioRxiv - Genomics 2024Quote: ... and polyA mRNA sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L and E7765L) per the manufacturer’s protocols ...
-
bioRxiv - Genomics 2024Quote: Adult worm Illumina sequencing libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Cat: E7805L) and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: cDNA was synthesized from the total RNA samples using either the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA), following the standard protocol with Random Primer Mix ...
-
bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 minutes in PBS containing the primary antibodies: rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs, Hangzhou, China), mouse monoclonal anti-tubulin β3 (801201 ...
-
bioRxiv - Microbiology 2019Quote: ... and detected using anti-MBP antibodies (New England Biolabs).
-
bioRxiv - Developmental Biology 2019Quote: ... incubated overnight with anti-SNAP antibody (NEB, Ipswich, MA), washed and probed with goat-anti-rabbit horseradish peroxidase secondary antibody (Invitrogen ...
-
bioRxiv - Developmental Biology 2019Quote: ... and detected using the anti-MBP-HRP antibody (NEB). The bound protein (~42kDa ...
-
bioRxiv - Microbiology 2024Quote: ... and detected using anti-MBP antibodies (New England Biolabs). As a negative control ...
-
bioRxiv - Neuroscience 2023Quote: ... TCF/LEF family antibody sampler kit (New England BioLabs), NFAT1 (New England BioLabs) ...
-
bioRxiv - Molecular Biology 2022Quote: Anti-M13-pIII monoclonal antibody (E8033S, New England Biolabs), sheep anti-mouse IgG (H/L):HRP (AAC10P ...
-
bioRxiv - Plant Biology 2023Quote: ... after which an anti-p42/p44-erk antibody (NEB) was employed to detect the activated MAPKs on western blots.
-
bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Genomic libraries were prepared using the NEBNext® Ultra TM II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, United States) using reagents at half volumes following Hale et al ...
-
bioRxiv - Genomics 2022Quote: ... were repaired and 3’-adenylated with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA). Sequencing adapters provided by Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd ...
-
bioRxiv - Genomics 2021Quote: ... were repaired and 3’-adenylated with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, MA, USA). Adapters with barcode provided by Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd ...