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Citations for New England Biolabs :
2851 - 2900 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... End point PCR analysis (Taq 2X Master Mix, NEB) was performed using 300 ng DNA template and 2-LTR circle primers ...
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bioRxiv - Microbiology 2023Quote: ... Each gene was PCR-amplified (Phusion; New England Biolabs) along with ∼300 bases 5’ of the translation start site to include the native promoter using primers designed with 16- 20 bases complementary to the gene of interest and 18-22 bases that overlap the NcoI site in pMV306hyg (Table S4) ...
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bioRxiv - Immunology 2023Quote: ... 25μl NEBNext High-Fidelity 2x PCR Master Mix (NEB), 2.5μl Nextera XT V2 i5 primer and 2.5μl Nextera XT V2 i7 primer (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and PCR-amplified 2.5 mM DNTPs (NEB, CAT#N0447S), 10X PCR buffer (Klentaq ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR products were treated with DpnI (NEB) to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara) ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... or Phusion High-Fidelity PCR Master Mix (NEB, M0531S). After agarose gel purification using columns (QIAGEN) ...
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bioRxiv - Cell Biology 2024Quote: ... All PCRs were made using Q5 DNA polymerase (NEB). All constructs were sequence verified ...
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bioRxiv - Neuroscience 2024Quote: ... PCR fragments were amplified using Q5 polymerase (M0494S, NEB). Both vectors and PCR fragments were purified using gel electrophoresis and gel extraction (28706 ...
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bioRxiv - Neuroscience 2024Quote: ... were amplified by PCR employing Q5 polymerase (NEB#M0494). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with Phusion HF DNA polymerase (NEB) with the following thermocycling conditions ...
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bioRxiv - Developmental Biology 2024Quote: PCR was with Phusion high fidelity DNA polymerase (NEB). PCR product was TA tailed with TAQ and TopoTA cloned into pCR4 ...
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bioRxiv - Genomics 2024Quote: ... while the PCR amplicon was digested with BsaI (NEB). These were purified using the DNA Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µl NEBNext HiFi 2× PCR Master mix (NEB) was immediately added and mixed before proceeding to PCR cycles (58 °C for 5 min (gap filling) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently sent for either library preparation and RNA-sequencing to Genewiz or cDNA preparation via a two-step process beginning with Lunascript RT Supermix Kit (#E3010, New England Biolabs). qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003 ...
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bioRxiv - Molecular Biology 2021Quote: ... The mRNA abundance levels were determined using 200 ng total RNA for each sample in technical replicates using Luna Universal One-Step RT-qPCR kit (New England BioLabs) and SYBR Green as detection agent and gene-specific primers (Table S2) ...
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bioRxiv - Genomics 2020Quote: Synthetic SARS-CoV-2 RNA templates were serially diluted and amplified by RT-LAMP using the WarmStart LAMP Kit (NEB). LAMP primers were added to a final concentration of 0.2µM for F3 and B3 ...
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bioRxiv - Biochemistry 2020Quote: ... The coupling reaction was allowed to proceed at RT for 45 minutes and was followed by addition of apyrase (0.0125 Units, NEB Inc.) to hydrolyse residual GDP and maintain the high-affinity nucleotide-free state of Gs ...
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bioRxiv - Pathology 2021Quote: ... β-ENaC/SCNN1B (Hs00165722_m1) and γ-ENaC/SCNN1G (Hs00168918_m1) were performed with the Luna Universal Probe One-Step RT-qPCR Kit (NEB, E3006L). Expression of each gene was normalized to 18S (Hs99999901_s1) ...
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bioRxiv - Bioengineering 2021Quote: ... Human codon-optimized retron RTs were synthesized and inserted into EcoRI-digested pHG023 backbone or SmaI-digested pHG077 backbone by NEBuilder (New England Biolabs). The cloning of plasmids for expressing 5’ rgRNA and 3’ rgRNA were based on pHG215 backbone ...
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bioRxiv - Bioengineering 2020Quote: ... We performed assays for the E and RNase P genes separately in 20 μL reaction volumes using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs). The final concentrations of primer and probe were 400 and 200 nM ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (EV Table 1 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (Forward 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler or an Applied Biosystems StepOnePlus system ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... were amplified in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler ...
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bioRxiv - Biochemistry 2021Quote: ... 5’-TAATCAGACAAGGAACTGATTA-3’ (Forward) and 5’-CGAAGGTGTGACTTCCATG-3’ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler ...
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bioRxiv - Bioengineering 2021Quote: ... RNA was purified using the Macherey Nagel RNA extraction kit following manufacturer’s instruction and viral RNA uptake was quantified using the Luna universal One-Step RT-qPCR kit (NEB; E3005).
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bioRxiv - Microbiology 2020Quote: Complementary DNA (cDNA) synthesis and qPCR were carried out using LunaScript RT Supermix and Luna UniveRsaL qPCR Master Mix (New England BioLabs), respectively ...
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bioRxiv - Microbiology 2020Quote: ... by PCR amplification of cDNA generated using reverse transcribed from total cellular RNA isolated from C636 Aedes albopictus cells using Protoscript II RT (NEB) and oligo-dT primers (IDT) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified with 250 ng of RNA inputs using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), using real-time RT-PCR primer/probe sets 2019-nCoV_N1 (CDCN1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... the CometChip was washed three times with PBS before being incubated for 1 h at RT in FPG (M0240S New England Biolabs) diluted 1:104 in PBS ...
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bioRxiv - Genomics 2022Quote: ... Poly-A mRNA was eluted in 36 μl of nuclease free water and 12 μl was reverse transcribed in a 30 μl reaction using the Template Switching RT Enzyme Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then diluted to 20 ng/uL and 20-60 ng of RNA was used for qPCR using Luna universal one-step RT-qPCR kit (NEB) as per manufacture’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... according to the manufacturer’s recommendations and subjected to ligation (2h at RT) with a pre-adenylated 3’ linker (2µM final) and a truncated T4 ligase (NEB M0373L). Ligated RPFs of 50 – 70 nucleotides (nt ...
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bioRxiv - Developmental Biology 2022Quote: Samples included 1) cDNA generated from >200nt RNA extracted previously from the 12h embryo sample (LunaScript RT Mastermix Kit, New England Biolabs). 2 ...
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bioRxiv - Genomics 2022Quote: RNA samples from 2 differentiation time courses with 5 time points were used to synthesize full-length barcoded cDNA libraries using the Template Switching RT Enzyme Mix (NEB). Libraries were prepared using Pacific Biosciences protocol for cDNA Sequence Capture Using IDT xGen® Lockdown® probes (https://eu.idtdna.com/site/order/ngs) ...
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bioRxiv - Immunology 2022Quote: ... and nCoV_IP4-14084Probe(+) TCATACAAACCACGCCAGG [5’]Hex [3’]BHQ-1 19) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2021Quote: ... by PCR amplification of cDNA generated using reverse transcribed from total cellular RNA isolated from C636 Aedes albopictus cells using Protoscript II RT (NEB) and oligo-dT primers (IDT) ...
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bioRxiv - Physiology 2021Quote: ... RNA (0.5-1 μg) was reverse-trancribed to cDNA using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). TaqMan gene expression assays with FAM-labeled probes were purchased from ThermoFisher Scientific (Carlsbad ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
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bioRxiv - Microbiology 2020Quote: ... separate reactions were performed for the quantification of SARS-CoV-2 N and E gene transcripts as well as cellular RNaseP for normalization using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) on a Bio-Rad CFX384 Touch system ...
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bioRxiv - Genomics 2021Quote: All samples that met the previous criteria were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Strand-specific CHIKV RNA primers were chosen from previous work [54] to amplify nucleotides 1-1560 of the CHIKV genome and used in reverse transcription reactions with the LunaScript8482 RT SuperMix Kit (NEB), followed by qPCR using the 2x qPCRBIO SyGreen Blue Mix (PCRBIOSYSTEMS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then subjected to library RT using random octamers (63) and to cDNA amplification thereafter using the Phusion High-Fidelity DNA Polymerase (New England Biolabs). A forward primer sequence against the 5’ Illumina adapter (63 ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1-2 μg) was reverse-transcribed using LunaScript™ RT SuperMix Kit (New England Biolabs, Catalog No; E3010S). Quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA (isolated of supernatant of Omicron isolate of RD infected cells) by LunaScript RT SuperMix (NEB). PCR reactions with Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...