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Citations for New England Biolabs :
2651 - 2700 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... ChIP-Seq was performed using libraries prepared with the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The unique barcoded region was amplified from 500ng genomic DNA with 16 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in30 ...
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bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Germany). To assess quality and size of the libraries ...
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bioRxiv - Genetics 2023Quote: ... strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A ...
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bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Microbiology 2023Quote: ... A linker was ligated to the ends of DNA using the NEBNext Ultra II Ligation Module (New England Biolabs, Cat# E7595). The junction between the 3’LTR of HIV-1 and host genomic DNA was amplified using primers targeting the 3’LTR and linker regions.
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bioRxiv - Genomics 2023Quote: ... Beads were washed with 200 μl buffer EB and resuspended in 400 μl of Post-TdT PCR mix (1x NEBNEXT Ultra II Q5 Master Mix (NEB, M0544L), 0.5 μM post-TdT-poly(C)12-S Primer ...
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bioRxiv - Genomics 2023Quote: ... Hi-C library was purified by adding 80 μl Ampure beads and amplified in two 100 μl pre-amplification reactions (50 μl 2x NEBNEXT Ultra II Q5 Master Mix (NEB, M0544L), 5 μl 10 μM Nextera-P5-pre-Primer ...
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bioRxiv - Immunology 2023Quote: ... 250 ng of input DNA was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequencing adaptors were ligated using NEBNext Multiplex Oligos for Illumina (#E7500S ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in a total volume of 50 μL with 4.0 μg genomic DNA per reaction using NEBNext Ultra II Q5 Master Mix (New England Biolabs, Ipswich, MA) (98 °C for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared by the Cornell TREx facility using NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA) with ribosomal RNA depletion ...
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bioRxiv - Genomics 2023Quote: ... RNA-seq library preparation was performed with the NEBNext Ultra II RNA Library Prep Kit and sequenced with the Illumina platform (NEB E7770S). Ribosomal RNAs (rRNAs ...
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bioRxiv - Cancer Biology 2023Quote: ... genomic DNA was isolated from bulk tumor-bearing lung tissue followed by PCR amplification of the sgID-BC region from 32 μg of bulk lung genomic DNA using Q5 Ultra II High-Fidelity 2x Master Mix (New England Biolabs, M0494X). Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645S). Sequencing was performed on an Illumina HiSeq 3000 ...
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bioRxiv - Genetics 2023Quote: ... Subtraction of poly-A RNA using NEBNext Ultra II with a Poly-A Selection kit (New England Biolabs, Ipswich, MA, USA) and poly-A RNA sequencing were performed at Admera Health (South Plainfield ...
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bioRxiv - Genetics 2022Quote: ... Libraries were prepared from 500ng of the total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) with Poly-A mRNA magnetic isolation (NEB #E7490 ...
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bioRxiv - Genetics 2023Quote: ... Then the sequencing library was generated by using the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.2-2.1 μg were used as a template to generate a sequencing library with the NEBNext Ultra II Directional PolyA mRNA (NEB, E7760S). The Advanced Sequencing Facility of the Francis Crick institute used an Illumina HiSeq 4000 to perform single end 1 × 75 bp sequencing.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: 500 ng of RNA was processed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB) with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... DNA was used to prepare a short reads library using NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, England) and sequenced on Illumina HiSeq X instrument (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... 125 ng of fragmented DNA was used to prepare each DNA library following the NEBNext Ultra II library prep kit for Illumina (NEB, Inc.) until the loop-adaptor ligation step ...
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bioRxiv - Genomics 2022Quote: ... Library preparation on samples was conducted using manufacturer’s protocols for NEBNext Ultra II Dna Library Prep Kit (New England BioLabs, CAT#E7645) and NEB Multiplex Dual Index oligos (New England BioLabs ...
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bioRxiv - Immunology 2023Quote: ... The double-stranded cDNAs were end-repaired using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, E7546S). Sequencing adapters were ligated to the end-repaired strands using Blunt/TA Ligase Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. No E7645S and E7335S) and amplified using 14 PCR cycles following the manufacturer’s instructions with slight modifications as previously described by us in detail elsewhere25 ...
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bioRxiv - Physiology 2023Quote: ... The gel-purified PCR products were cloned into the EcoRI- digested pBluescript II SK(+) vector using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621X). The mixture of pU6b-sgRNA and targeting vector was microinjected into w1118 ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was normalized for cDNA conversion using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Massachusetts, USA) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... the library preparation process was started from the “End-prep” step of the aforementioned protocol using NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs,NEB). A Blunt/TA Ligase Master Mix (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Library preparation was done according to NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins ...
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bioRxiv - Microbiology 2023Quote: ... Sequence libraries were prepared with the NEBNext Ultra II FS DNA Library Prep 136 kit (New England Biolabs GmbH, Frankfurt, Germany) and sequenced with the NovaSeq6000 (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was quality checked using Bioanalyser (RIN>9) and libraries generated using NEBNext Ultra II Library Prep Kit (NEB, E7770) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The directional RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library prep for Illumina kit (NEB, E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7765), and a minimum of 20 million raw reads were obtained ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs; #E7760). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Microbiology 2023Quote: ... The NGS library preparation was carried out using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, USA), which performs fragmentation ...
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bioRxiv - Molecular Biology 2023Quote: ... adaptor ligation and PCR indexing was performed on the denatured amplicons using NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, UK). The resulting FASTQ files from RNP treated samples for each of the off target amplicons were analysed for indels through CRISPResso2 webtool [45] by comparing them with untreated samples.
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bioRxiv - Genomics 2023Quote: The purified cDNA was PCR amplified for 6 cycles to generate dsDNA with NEBNext Ultra II Q5 High-Fidelity 2X Master Mix (NEB, M0544) and 0.5 uM PCR primers with unique dual index using the following PCR cycles:
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bioRxiv - Molecular Biology 2023Quote: ChIP libraries were generated with input and pulldown DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645L) for sequencing on an Illumina Nextseq with 75-bp read length and single-end.
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bioRxiv - Molecular Biology 2023Quote: ... Nascent RNA was then fragmented at 85C for 10 min and converted to cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (NEB, E7771) and NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... of extracted RNA (either from whole-cell lysates or sucrose gradient fractions) was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560L) according to the manufacturer instructions with some modifications ...
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bioRxiv - Immunology 2023Quote: ... The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.) with AMPure XP (Beckman Coulter Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 3 - 10 × 106 EB-derived CD71+ were processed per biological replicate and libraries of sufficient quality were indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (New England Biolabs) according to the provided instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, E7760L) by following manufacture’s protocol ...