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Citations for New England Biolabs :
2451 - 2500 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... RNA-Seq libraries (400 bp) were created using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L). Samples were individually indexed using the NEBNext Multiplex Oligos for Illumina (NEB #E6609S) ...
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bioRxiv - Developmental Biology 2020Quote: Libraries for DNA sequencing were prepared according to the instructions accompanying the NEBNext DNA Ultra II kit (catalog # E7645S; New England Biolabs, Inc). Libraries were sequenced on the NextSeq 500 following the manufacturer’s protocols ...
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bioRxiv - Genomics 2019Quote: ... DNA libraries were prepared at the Fundación Parque Científico de Madrid (FPCM) using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) and purified with Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2019Quote: ... Up to 10 ng of DNA was used for the library construction using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645). Sequencing was performed on NextSeq500 (Illumina ...
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bioRxiv - Genomics 2020Quote: ... ChIP-seq libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, #E7103). The detailed ChIP-seq protocol can be found in Supplementary materials.
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bioRxiv - Genetics 2021Quote: ... Custom inverse complementary adapters that had inverse complementary terminal modifications to ensure unidirectional ligation (3’-T overhang and 5’ phosphorylation) were ligated onto both ends of the respective subsets using the Ultra II Ligation Module (NEB, USA) according to manufacturer’s instructions and purified with 1:1 bead cleanup (Figure 3) ...
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bioRxiv - Genetics 2020Quote: ... 50 pg of repaired and adapter-ligated products were amplified for 19 cycles with the NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544) and dual-indexed with custom IDT Ultramer primers ...
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bioRxiv - Genomics 2021Quote: ... 200ng of sonicated fragments were used to make NGS (next generation sequencing) libraries using NEBNext Ultra II DNA Library Prep (New England BioLabs, E7645S) in combination with methylated adaptors (NEB ...
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bioRxiv - Genomics 2021Quote: ... for 30 min at 37 °C and PCR amplification to add the Illumina smallRNA barcodes and handles by adding 25 μl of NEBNext Ultra II Q5 Master Mix (NEB, M0492L), 11 μl Nuclease free water and 2 μl of RP1 and RPIx primers (10 μM) ...
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bioRxiv - Genomics 2021Quote: ... The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760). The library was sequenced on illumina Miseq (75 bp paired-end sequencing) ...
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bioRxiv - Genomics 2021Quote: ... for 1 hr at 25 °C then converted to first strand cDNA with random priming and the ProtoScript II reverse transcriptase mix (NEB E6560). Amplification reactions from this cDNA were performed with the 5’ PCR primer and a reverse primer corresponding to the target sequence using LongAmp Taq polymerase (NEB M0287) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library of each sample was prepared separately with the NEBNext® Ultra (tm) II FS DNA Library Preparation Kit and the Unique Dual Indexing kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of that advised by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... The elution product was directly used for second-strand synthesis using reagents from the NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760). 8 μL of Second Strand Synthesis Reaction Buffer ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were prepared at Génome Quebec (Montreal, QC, Canada) with the Ultra II NEB DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Library quality and size distribution was determined on a high-sensitivity DNA chip on the Agilent 2100 bioanalyzer system ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were prepared on a template of randomly fragmented mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA). Since 8 samples out of 20 initially contained < 200 ng of total RNA ...
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bioRxiv - Synthetic Biology 2021Quote: Illumina sequencing libraries were generated from 100-200 ng BAC DNA using NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs E7805S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared using the rRNA-depleted RNA as input for NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA). Libraries were pooled and submitted for 2 × 150 bp paired-end sequencing on an Illumina NextSeq High-Output platform at the Center for Genome Sciences and Systems Biology at Washington University in St ...
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bioRxiv - Developmental Biology 2021Quote: ... using 200 ng of sheared gDNA and 10 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The DNA libraries were indexed with unique dual barcodes (8bp long) ...
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bioRxiv - Biochemistry 2021Quote: Illumina sequencing libraries were prepared from 1ng to 10ng of purified CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs # E7645) as previously (Liu et al ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA, USA inc.) for Illumina following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... libraries were prepared from purified DNA using “NEBNext Ultra II DNA Library Prep Kit for Illumina” from New England BioLabs (NEB, #E7645), as per the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genetics 2020Quote: ... Followed by a Dual index library prep with the DNA NEBNext® Ultra™ II Library Prep Kit (New England Biolabs), using a quarter of the recommended volumes with the Dual Index Primers Set ...
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bioRxiv - Genetics 2021Quote: ... Libraries for sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material ...
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bioRxiv - Microbiology 2021Quote: ... while the Zymo Mock sample was prepared with NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, USA) following the manufacturer’s protocols and sequenced using the Illumina MiSeq platform.
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bioRxiv - Microbiology 2021Quote: ... the sgRNA-encoding region was amplified from 500 ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master Mix (NEB #M0544L). Each PCR reaction contained a pool of forward primers (0.5 μM final concentration ...
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bioRxiv - Genomics 2021Quote: Double-stranded single-indexed libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645S) and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S ...
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bioRxiv - Microbiology 2021Quote: ... and all of the point mutants were constructed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme, C214) and DpnI endonuclease (NEB, R0176S). Primers used for plasmids construction were listed in Supplementary table 1 and table 2 ...
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bioRxiv - Microbiology 2021Quote: ... (Leiden, The Netherlands) using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) and the NextSeq 500 platform (paired-end ...
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bioRxiv - Microbiology 2020Quote: ... and libraries for RNAseq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... cDNA was generated from 1ug of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560). RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB, USA). Sequencing was performed using a NextSeq HIGH 150 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... was used per sample for the library preparation using the NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760L). The libraries were then sequenced on Illumina HiSeq 2500 sequencing platform using 2×100 bp sequencing format.
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bioRxiv - Molecular Biology 2022Quote: ... all of the eluted DNA was used to construct NextSeq DNA libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, #E7645S). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (New England Biolabs (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA) for Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... were prepared using the NEBNext® Ultra II FS DNA Library Prep Kit for Illumina with NEBNext Sample Purification Beads (New England BioLabs). This protocol includes a short PCR amplification (5-7 cycles ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 24 h bacterial culture in LB without NaCl was isolated from bacteria using TRIzol reagent (HiMedia) and cDNA synthesized using ProtoScript® II Reverse Transcriptase (NEB). qRT-PCR was performed using PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The sgRNA-encoding region was amplified from 500ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in ...
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bioRxiv - Evolutionary Biology 2022Quote: ... apterus libraries were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, United States) at the Berlin Center for Genomics in Biodiversity Research (BeGenDiv) ...
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bioRxiv - Genetics 2022Quote: ... RNAseq libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA) and sequenced with the Illumina HiSeq instrument (Illumina Inc. ...
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bioRxiv - Plant Biology 2021Quote: ... Paired-end libraries of DNA were constructed by the NEBNext Ultra II DNA Library Prep for Illumina Kit (New England Biolabs; E7645L) with 2 × 150 bp with average insert size ~900 bp and sequenced on a NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA) and index codes were added ...
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bioRxiv - Immunology 2020Quote: ... Purified DNA was subjected to ChIP-Seq library preparation with NEBNext Ultra II DNA Library Prep Kit for Illuumina (NEB, #E7645) and sequenced by paired-end 75-bp sequencing on Next-Seq550 ...
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bioRxiv - Microbiology 2021Quote: ... RNA quality was assessed using the Agilent Bioanalyzer and appropriate samples were used for NGS library preparation with the NEBNext Ultra II Directional RNA kit (NEB E7760) and sequenced with 50 bp paired-end reads and 30 mio reads per sample on the Illumina HiSeq 2500 ...
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bioRxiv - Developmental Biology 2020Quote: ... and libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB; E7760), as directed by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... the purified DNA was used as library construction with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L). High-throughput sequencing was carried out using Illumina Hiseq-PE150 by Novogene Corporation (Beijing ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng rRNA-depleted RNA from each nuclear fraction was used with the NEBNext Ultra II Directional library kit (NEB E7760S) to prepare RNA-seq libraries ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were constructed with the NEBNext RNA Ultra II Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, United States) from 1 µg of purified RNA according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Library preparation of immunoprecipitated DNA fragments was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated from RNA using 4 µL of 5X ProtoScript II buffer (cat. n° M0368L, NEB, New England Biolabs, USA), 2 µL of 0.1 M Dithiothreitol (cat ...