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Citations for New England Biolabs :
2501 - 2550 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cDNA was generated from RNA using 4 µL of 5X ProtoScript II buffer (cat. n° M0368L, NEB, New England Biolabs, USA), 2 µL of 0.1 M Dithiothreitol (cat ...
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bioRxiv - Neuroscience 2021Quote: ... non-stranded cDNA libraries were constructed using poly(A) mRNA enrichment and the NEBNext Ultra™ II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, E7770S). RNA and library qualities were confirmed using Qubit Flourometric Quantification (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Novogene prepared a PCR free Illumina sequencing library using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA), with the manufacturers protocol modified to give a 500 bp – 1 kb insert size ...
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bioRxiv - Microbiology 2021Quote: ... RNA passing quality control was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit with polyA selection as per the manufacturer’s instructions (NEB, Ipswich, MA). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Purified DNA was applied to Tru-Seq library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). For NEB kit ...
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bioRxiv - Systems Biology 2022Quote: Sequencing libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Cat#E7765L) in 96-well plates ...
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bioRxiv - Genomics 2022Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB, E7645S). All end preparation ...
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bioRxiv - Molecular Biology 2020Quote: NEBNext RNA-Seq library preparation starting from 10ng and 200ng total RNA was performed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Cat.No.E7770). Single cell SmartSeq2 library was constructed as previously reported [2].
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bioRxiv - Evolutionary Biology 2019Quote: ... We prepared libraries using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (New England BioLabs, Ipswich, Massachusetts). Final fragment sizes and concentrations were confirmed using a TapeStation 2200 system (Agilent ...
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bioRxiv - Genetics 2019Quote: ... RNA was isolated from cells using the Direct-zol™ RNA Miniprep Kit and used to generate cDNA (Protoscript II, NEB). Protein was isolated from cells as described above.
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bioRxiv - Genomics 2019Quote: ... were PCR-amplified (2 PCR reactions, 12 cycles) using Illumina adapter-specific primers and NEBNext® Ultra II Q5 Master Mix (NEB). After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Library preparation was conducted using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs, USA) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 48hr APF TRAP libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, product E7760S) and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB ...
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bioRxiv - Genetics 2020Quote: ... The purified DNA (200 ng) was subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Genetics 2020Quote: Libraries were prepared from 2 ng of DNA using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were created using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and sequencing performed on an Illumina NextSeq 500 using a NextSeq High Output Kit ...
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bioRxiv - Genomics 2020Quote: ... 700 nanograms of extracted DNA was utilized as starting material in NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs, USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with random primers using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) with NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Microbiology 2021Quote: ... then an RNAseq library was prepared with the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was then performed in single read mode on a HiSeq 3000 system ...
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bioRxiv - Microbiology 2021Quote: ... and 500ng DNA was used as input for the NEBNext Ultra II FS DNA Library Prep kit (New England Biolabs, USA), following the manufacturer’s instructions with modifications as follows ...
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bioRxiv - Systems Biology 2019Quote: ... 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760L) and included polyA-enrichment using NEBNext Poly(A ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared following the protocol from NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770S) and indexes (NEB #E7335S and #E7500S).
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bioRxiv - Evolutionary Biology 2019Quote: ... Library preparation was done using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt, Germany) and the protocol for use with inputs of ≥ 100 ng DNA ...
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bioRxiv - Genomics 2021Quote: ... cDNA synthesis by RT was performed immediately after DNaseI digestion of placental RNA with the ProtoScript®II First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers ...
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bioRxiv - Genomics 2021Quote: ... 250–500 ng extracted RNA was used to prepare libraries with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs #E7760S) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA per sample was processed using the NEBNext Ultra II Directional RNA library prep kit from NEB (E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Genetics 2020Quote: ... An Illumina DNA library was prepared with the NEBNext® Ultra™ II FS DNA Library Prep Kit (NEB, Ipswich, USA) and multiplex barcoded with NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2 ...
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were generated with 300 ng of total RNA using NEB Next Ultra II RNA Library prep kit (NEB, E7770) according to the vendor’s protocol ...
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bioRxiv - Genomics 2021Quote: We generated three replicates of +/- doxycycline-treated RNA-sequencing libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765S). We quantified and assessed the quality of the libraries with an Agilent 2200 Tapestation instrument ...
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bioRxiv - Microbiology 2021Quote: ... OtownIsak and SummerBoyErik were prepared using NEBNext® Ultra II FS library prep kit for Illumina (New England Biolabs, Ipswich, USA), while the sequencing library for Hovsa was prepared with Nextera® XT DNA Kit (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and both PCRs were carried out in 5μl total volume using the NEBNext Ultra II Q5 Mastermix (New England Biolabs, Ipswich, MA). The final amplicons were again purified ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries for next generation sequencing were constructed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) and sequenced on a HiSeq2500 Sequencer ...
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bioRxiv - Microbiology 2021Quote: ... were diluted in 45 μL nuclease-free water before the end repair and dA tailing were performed with the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, USA). 1.5 μL end-prepped DNA was transferred to the next reaction directly and the barcodes derived from EXP-NBD196 (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 μL end-prepped DNA was transferred to the next reaction directly and the barcodes derived from EXP-NBD196 (Oxford Nanopore Technologies, UK) were ligated with NEBNext Ultra II Ligation Module (New England Biolabs, USA). After the clean up of the pooled different barcoded samples jointly with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) according to the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... Twenty ng of purified PCR product was used as input for library construction using the NEBNext Ultra II FS DNA Library Prep Kit (NEB, E6177S) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription reactions were performed with 6 μl of purified RNA and random primers using the NEB ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The G1 and S/G2 population were sorted using FACSAria II cell sorter (Becton Dickinson) in PBS supplemented with RNase inhibitor (NEB, M0314L). Sorted samples were processed for scRNA-seq or cDNA-seq.
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bioRxiv - Developmental Biology 2020Quote: ... and 1μg of total RNA from each sample was reverse transcribed into cDNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Novogene prepared a PCR-free Illumina sequencing library using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA), with the manufacturers protocol modified to give a 500 bp – 1 kb insert size ...
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bioRxiv - Evolutionary Biology 2021Quote: ... End repair and dA-tailing of the fragmented DNA was done with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB E7546S). A reaction mix was made by adding 7 ul of NEB Ultra II end-prep reaction buffer ...
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bioRxiv - Immunology 2020Quote: ... RNA sequencing libraries were prepared from 500 ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit with Poly-A mRNA magnetic isolation (NEB #E7490) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... For the library preparation the NEBNext® Ultra™ II DNA Library Prep Kit from NEB (New England Biolabs, Ipswich, MA) was used ...
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bioRxiv - Microbiology 2019Quote: ... the initial g-TUBE shearing step was omitted and potential nicks in DNA and DNA ends were repaired in a combined step using NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs, USA) and doubling the incubation time ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs; Ipswich, MA, USA), and the library quality was assessed on Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645) according to manufacturer instructions with a few modifications ...
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bioRxiv - Microbiology 2021Quote: ... The ChIP libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina (Catalog: E7645S, New England Biolabs). The samples were subjected to various enzymatic steps to repair the ends and tailing with dA-tail followed by an adapter ligation ...
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bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from total RNA using polyA selection followed by the NEBNext Ultra II Directional RNA Library Prep Kit protocol (New England Biolabs, E7760S). Sequencing was performed on Illumina HiSeq 2500 instrument resulting in approximately 30 million of 50 bp reads per sample ...
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bioRxiv - Immunology 2021Quote: ... captured mRNA was processed for Next Generation Sequencing (NGS) using the NEB Next Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, UK). Quality was assessed on the Agilent 2100 Bioanalyser using the High Sensitivity DNA Kit (Agilent ...