Labshake search
Citations for New England Biolabs :
2551 - 2600 of 6440 citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instructions.29 RNA reverse transcription and first strand cDNA synthesis were carried out using chain-specific reverse primers30 with ProtoScript® II First Strand cDNA Synthesis Kit (NEB). The resulting cDNA was used as template for preparing the sequencing library using 5’ multiplex PCR as previously described.31 The PCR products corresponding to the library amplification were purified on BluePippin with size cutoff around 500 bp ...
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bioRxiv - Genomics 2021Quote: ... and the fragmented DNA was subjected to library preparation with NEBNext® Ultra II DNA Library Prep Kit for Illumina (E7645S, New England Biolabs). Sequencing was carried out on Illumina NovaSeq 6000 with 150bp paired end mode.
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bioRxiv - Genomics 2020Quote: ... The reaction mixture was composed of a PCR ready mix (using NEBNext® Ultra™ II Q5® Master Mix, NEB), lysate product and a final primer concentration of 1uM each ...
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bioRxiv - Microbiology 2020Quote: ... The sequencing libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, MA, USA). Coverage values are reported in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... The immunoprecipitated DNAs were subsequently processed to generate a DNA library using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, # E7645L) and NEBNext® Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries for RIP-TRIBE and polysome were prepared in triplicates from 150 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBnext rRNA depletion Kit (NEB #E6310L). cDNA libraries were sequenced on an Illumina NovaSeq6000 using S-prime or S1 flow cells with paired-end 150-bp reads ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously (33) ...
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bioRxiv - Molecular Biology 2022Quote: ... for each co-expressed DF-APOBEC1 protein using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) with NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... equal amount of DNA (∼2 ng) was used as an input for NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350) ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Biochemistry 2022Quote: ... RNA from each sample was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was carried out with a standard oligo-dT primer protocol using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560). RNA concentrations were normalized between samples prior to reverse transcription ...
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bioRxiv - Cancer Biology 2022Quote: ... and library preparation was done using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).56 Paired-end 150 bp read sequencing was performed ...
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bioRxiv - Genomics 2022Quote: ... The “End-prep” was performed with 48 μl of sheared DNA in the reaction mixture using NEBNext Ultra II End Repair/dA-Tailing Module and NEBNext FFPE DNA Repair Mix (New England Biolabs (NEB), cat# E7546 ...
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bioRxiv - Genetics 2022Quote: ... and DNA library preparation was performed using NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads Kit (NEB, #E6177). Whole-genome sequencing was performed using an Illumina NextSeq instrument ...
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bioRxiv - Genetics 2022Quote: ... Libraries were produced with the Ultra II DNA Library prep kit according to manufacturer’s instructions (New England Biolabs, cat. no. E7645L). Bisulfite conversion of DNA was carried out according to manufacturer’s protocol (Zymo ...
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bioRxiv - Genomics 2022Quote: ... IPs and INPUTS were then quantified and NGS libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA libraries were generated using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (New England BioLabs, Ipswitch, MA). Resulting libraries were sequenced on a NovaSeq 6000 at a depth of 30 million reads/sample ...
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed from these sheared DNA samples using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S) ...
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bioRxiv - Immunology 2022Quote: ... 200 ng of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs Inc). Libraries were sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: Extracted DNA was used for DNA library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were end-repaired and A-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs Ltd) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries for next-generation sequencing were prepared using the NEBNext Ultra(tm) II Directional RNA Library Prep kit with Sample Purification Beads (NEB, E7765S). Libraries were indexed using NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 2 (E7335S and E7500S) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA samples from empty vector and sgRNA2 MEFs were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760), as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... The eluted DNA was used for sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645). Biotinylated DNA fragments were enriched by Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: Illumina library was prepared at GIGA Genomics (University of Liege, Belgium) using NEBNext Ultra II DNA library prep kit (New England BioLabs, US) and libraries were sequenced on the Illumina NextSeq500 sequencer for the generation 10 M of paired-end reads (2 x 150 base pair ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB; E7765), and a minimum of 20 million raw reads were obtained ...
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bioRxiv - Synthetic Biology 2022Quote: ... Purified DNA was eluted in 30 μl of Ultrapure water and 10ng was inputted into the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR programme ...
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bioRxiv - Molecular Biology 2022Quote: ... and 500 ng of the gDNA was subjected to DNA library preparation using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England Biolabs, E7805S) following manufacturer’s instructions (protocol for inputs >100 ng) ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared following the instruction from NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and indexes (NEB #7335S ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 10 ng of DNA was used for the library construction using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645). Sequencing was performed on NextSeq 500 (Illumina).
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bioRxiv - Molecular Biology 2022Quote: ... One µg of RNA together with oligo(dT) primers were used for the cDNA synthesis using the ProtoScript®II first strand cDNA synthesis kit (New England Biolabs) according to the manufacture’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacturer’s instructions and the recovered poly(A) selected RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation of immunoprecipitated DNA fragments was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Plant Biology 2022Quote: ... treatment and used for complementary DNA (cDNA) synthesis with a ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs). To clone the coding region of maize BABY BOOM (BBM ...
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bioRxiv - Plant Biology 2022Quote: ... and libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, Ipswish MA, USA). Libraries were then quantified using Qubit™ Fluorometer 4 and Fragment analyzer 5200 (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... 10-100 ng of DNA from each sample was carried forward for end-repair using the NEBNext Ultra II End repair/dA-tailing Module (NEB, E7546S). The DNA was mixed with 1X volume of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Cat. No. / ID: E7770) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc, Ipswich, Massachusetts, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF256, cTF257) and 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544) and ran the following thermocycling protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for small DNA fragments (25-75 bp) were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Cell Biology 2024Quote: ChIP-seq libraries were prepared independently from two ChIP biological replicates using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E6177L) according to manufacturer’s instructions with a few modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (catalog no. E7760L, NEB) and indexed with NEBNext® Multiplex Oligos for Illumina (catalog no ...
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bioRxiv - Biochemistry 2024Quote: ... library indexing was carried out with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat. E7645S) and sequenced on a NextSeq 1000 P2 cartridge (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification of the BC-sgRNA1-sgRNA2 region from 32 μg of bulk lung genomic DNA using Q5 Ultra II High-Fidelity 2× Master Mix (New England Biolabs, M0494X). Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter ...