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Citations for New England Biolabs :
1 - 50 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... TCA extract in Laemmli buffer was diluted 10-fold in 1X PMP buffer (NEB, Protein Metallo Phosphatases) supplemented with 1 mM Mncl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of RNA was treated with DNase (DNAse I RNas-free, NEB) for 10-15 minutes at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein (200 µg) was precleared with protein G magnetic beads (New England Biolabs) and incubated overnight (4° C ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was loaded into the cobalt affinity column (New England Biolabs) and eluted with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was used for standard library preparation using NEBNext PolyA mRNA magnetic isolation module (NEB, E7490). In brief ...
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bioRxiv - Microbiology 2022Quote: ... Proteins from the supernatant at 2 mg/mL were used for the Co-IP assay using Protein G magnetic beads (New England Biolabs) and a mouse monoclonal anti-HA antibody (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1–10 µg of RNA transcribed in vitro was treated with Escherichia coli poly(A) polymerase (5000 U/mL, New England Biolabs, M0276) at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... and ATF4 5ʹ UTR-nLuc-3XFLAG mRNAs were co-transcriptionally capped with the 3’-O-Me-m7G(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1411S). All viral IRES nLuc-3XFLAG mRNAs were co-transcriptionally capped with the A(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1406S).
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bioRxiv - Biochemistry 2023Quote: ... All viral IRES nLuc-3XFLAG mRNAs were co-transcriptionally capped with the A(5ʹ)ppp(5ʹ)G RNA Cap Structure Analog (NEB # S1406S).
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bioRxiv - Molecular Biology 2022Quote: ... DNA was transcribed into mRNA which was co-transcriptionally capped with the Anti-Reverse Cap Analog (ARCA) 3′-O-Me-m7G(5′)ppp(5′)G (NEB # S1411) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040) ...
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bioRxiv - Genomics 2021Quote: ... TF motif libraries in pGL4.10-Sasaki-SS (a) and pCpG-free-EF1α-SS (b) vectors were amplified using standard Illumina Universal and index primers (NEB #E7335S) and sequenced using standard Illumina chemistry ...
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bioRxiv - Neuroscience 2023Quote: ... AKT (9272, NEB, 1:5000); Phospo-AKT (9271 ...
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bioRxiv - Molecular Biology 2023Quote: (Single-stranded DNA oligos were synthesized by NEB Organic synthesis division): Top strand Gm6ATC (top M+)
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bioRxiv - Evolutionary Biology 2024Quote: ... A PCR master mix was employed with final concentrations of Standard ThermoPol buffer Mg-free (1x) (New England Biolabs, Ipswich MA, USA), dNTPs (0.2 mM each ...
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bioRxiv - Microbiology 2021Quote: ... RNA was eluted in RNase-free water and subsequently treated with Turbo DNase and purified using the Monarch RNA Cleanup Kit (50 µg) (NEB) and eluted in RNase-free water ...
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bioRxiv - Genetics 2021Quote: A 25 μL PCR master mix was set up as follows: 2.5 μL Standard Taq (Mg-free) Reaction Buffer (10x) (B9015 New England BioLabs Inc. Ipswich MA USA), 2.0 μL dNTP mix (10mM total ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Standard Gibson Assembly (NEB) cloning was used to construct linker variants PaFtsH2H1-link-32 ...
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bioRxiv - Molecular Biology 2023Quote: ... The custom made Pyrollo-dC adaptor (NEB Organic Synthesis Division, Ipswich MA), where all dCs are replaced with Pyrollo-dC ...
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bioRxiv - Neuroscience 2023Quote: ... Phospo-AKT (9271, NEB, 1:5000); cFOS (134122 ...
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bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... 7 µg (DNA mass) of NCPs (with 188bp NPS) or free DNA was incubated with 5 Kunitz units of MNase (NEB M0247) in a reaction buffer (30 mM Tris pH 8.0 ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of RNA was treated with RQ1 RNA-free DNase and retrotranscribed using either LunaScript® RT Supermix (New England Biolabs) or iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... standard Illumina primers (NEB #E7335S) and sequencing chemistry were used ...
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bioRxiv - Molecular Biology 2024Quote: ... 5000 CEU T4 DNA ligase (NEB, M0202L)) and rotated on a slow rotator for 3 hours or more at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Large (Klenow) Fragment (NEB, 5000 U/mL) was incubated at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... The soluble fraction of the lysate was used for affinity purification on HisPur Cobalt resin (Thermo, Cat#89964), Glutathione Agarose resin (Thermo, Cat#16100) or Amylose Resin (New England Biolabs, E8021), depending of the fusion tag ...
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bioRxiv - Bioengineering 2021Quote: ... standard Taq buffer (New England Biolabs), 2 µL of 20 µM of each primer ...
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bioRxiv - Bioengineering 2021Quote: ... Standards (low range ssRNA ladder, NEB) and 10 pmol of unligated tRNA sample were prepared in an equivalent buffer to the first ligation reaction (1X RNA Ligase 2 buffer (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... PCR using Standard Taq Polymerase (NEB) was performed for 30 cycles of (denaturation ...
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bioRxiv - Microbiology 2022Quote: ... Color Prestained Protein Standard (NEB, P7719) or Precision Plus Protein All Blue Prestained Protein Standards (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... standard Taq buffer (New England Biolabs), 0.5 unit of Taq polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... and standard Q5 reaction buffer (NEB). In an Eppendorf Mastercycler EP Gradient S ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Standard Taq (New England Biolabs #M0273) or Phusion (New England Biolabs #M0530 ...
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bioRxiv - Bioengineering 2024Quote: ... with the standard reaction buffer (NEB), 0.2 mM dATP/dGTP ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL of 5000 units/mL RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse antibodies against MBP (NEB E8032L, 1:5000) and RAD51 (Novus Biologicals 14b4 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μl of 5000 units/ml RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1X Standard Taq Reaction Buffer(NEB B9014S) and certain dosage of M13mp18 template according needs ...
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bioRxiv - Biochemistry 2022Quote: ... using a standard New England BioLabs (NEB) heat shock protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1X Standard Taq Reaction Buffer (NEB). The libraries were gel-purified using the E.Z.N.A Gel Extraction Kit (Omega Bio-Tek) ...
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bioRxiv - Microbiology 2023Quote: ... and a standard Phusion polymerase protocol (NEB). Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina Standard library construction (New England Biolabs) and sequencing (Illumina NovaSeq ...
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bioRxiv - Synthetic Biology 2024Quote: Standard Q5 high-fidelity DNA polymerase (NEB) PCR conditions and protocols were used for the DNA amplification ...
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bioRxiv - Biochemistry 2022Quote: ... using a standard Q5 (New England Biolabs) PCR reaction but holding the temperature at 72 degrees for 30’ ...
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bioRxiv - Molecular Biology 2023Quote: ... Taq DNA polymerase with standard buffer (NEB) was used with 25 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... which was co-injected with I-SceI (NEB) into the Tg(kdrl:CreERT2)cq24 embryos for transgenesis and screen ...
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bioRxiv - Biochemistry 2020Quote: ... and G(5′)ppp(5′)G RNA (GpppG) (NEB, # S1407S) are from New England Biolabs (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µg BSA (NEB), 9 mM DTT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA standards (ssDNA standards) were amplified from pRnc-gRNA DNA using Phusion High Fidelity DNA Polymerase (New England BioLabs) with the pWP252fluor reverse primer and forward primers annealing to the expected 5’ termini of the cleaved and uncleaved 5’UTR that would yield the desired size products ...