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Citations for New England Biolabs :
51 - 100 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... PNGase F (NEB/P0704L) and 20mM Tris buffer (pH=7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... For PNGase F (NEB) treatment ...
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bioRxiv - Bioengineering 2024Quote: ... PNGase F (P0710S NEB) was used ...
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bioRxiv - Immunology 2022Quote: Single mutations of the spike protein were generated via two PCR fragments of the spike ORF using high-fidelity Phusion polymerase (New England Biolabs, USA). The first fragment was generated via a generic forward primer (pCAGGS-5 ...
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bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... while 300 units of Peptide:N-Glycosidase F (PNGase F, P0704S, New England Biolabs) was added to the other set of tubes and incubated at 37 °C for 16 h in a Thermoshaker at 1500 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The Peptide-N-Glycosidase F (PNGase F) enzyme (New England Biolabs, catalog #: P0704L)-treated samples served as a control for unglycosylated α1 subunits ...
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bioRxiv - Microbiology 2024Quote: ... gladioli strain FDAARGOS_389 strain using Q5 High-Fidelity DNA polymerase PCR (New England Biolabs) following 20 µl final volume and reaction components mentioned in the manufacturer’s protocol (https://www.neb.com/en-us/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli BL21DE3 strain (C2527H, NEB). The culture was expanded and expression was induced at an OD600 of 0.7 with 0.4 mM IPTG ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strains DH5α (NEB C2987I) and NEB Turbo (NEB C2984I ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain (New England Biolabs). The purification protocol was identical to other samples with a couple of exceptions ...
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bioRxiv - Microbiology 2021Quote: ... coli strain (New England BioLabs) and co-transformed with pMW535 ...
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bioRxiv - Biophysics 2021Quote: ... coli strain (New England Biolabs). Cells were first grown in M9 minimal media with 200 mg/L ampicillin and 34 mg/L chloramphenicol at 37 °C and 180 rpm ...
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bioRxiv - Microbiology 2022Quote: ... coli strain SHuffle® (NEB) at 30 °C (for candidates C14 and derivatives ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli LEMO21(DE3) strain (NEB) as a fusion protein containing a (His)10-tagged cysteine protease domain (CPD ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli BL21(DE3) strain (NEB) at 18 oC as a fusion protein with glutathion-S-transferase (GST ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli LEMO21(DE3) strain (NEB) was transformed with a pET21b+ or pET29b+ plasmid encoding the synthesized gene of interest ...
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bioRxiv - Biochemistry 2019Quote: ... coli strain (NEB, Ipswich, MA) was used for protein expression ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Lemo21(DE3) strain (NEB) was transformed with a pET29b+ plasmid encoding the synthesized gene of interest ...
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bioRxiv - Biophysics 2021Quote: ... coli strain (New England Biolabs), respectively ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli strain (New England Biolabs). To ensure that N-terminal methionines were post-translationally cleaved from the nascent globin chains ...
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bioRxiv - Cell Biology 2023Quote: ... coli strain (New England Biolabs).
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... coli strain BL21(DE3) (NEB) grown at 37°C in LB supplemented with 50 μg/mL kanamycin ...
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bioRxiv - Genetics 2023Quote: ... Escherichia coli strain DH10beta (NEB) was grown at 37°C in LB (10 g tryptone ...
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bioRxiv - Biophysics 2023Quote: ... coli strain (New England Biolabs) was cultured in terrific broth at 37°C and induced using 0.4 mM IPTG for 16h at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain (New England Biolabs) was used for cloning and plasmid propagation.
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bioRxiv - Plant Biology 2023Quote: ... coli strain (New England Biolabs). Primers used for cloning are detailed in Supplemental Table 1.
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bioRxiv - Microbiology 2023Quote: ... coli BL21(DE3) strain (NEB) were used ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB 10β (NEB). L-arabinose (A3256-25 g) ...
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bioRxiv - Bioengineering 2024Quote: ... coli Shuffle T7 strain (NEB) was transformed with pET17b OVA-ZE ...
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) strain (NEB). Cells were grown at 37°C supplemented with 2XYT medium (Melford ...
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bioRxiv - Cell Biology 2021Quote: ... m6A-modified RNA spike-in controls (New England Biolabs) and a nontargeted region on the crRNA-targeted transcript ...
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bioRxiv - Neuroscience 2021Quote: ... Deglycosylation was performed with peptide-N-glycosidase F (PNGase F; New England Biolabs, USA) following the manufacturer’s instructions [28] ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... PNGase F (NEB, Beijing, China) was used for the deglycosylation analysis with an overnight incubation at 37°C.
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bioRxiv - Cell Biology 2020Quote: ... 1µL PNGase F (NEB, P0708L) and H2O as necessary to a final volume of 20µL ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μL PNGase F (NEB), and 5 μL water were added and the solution incubated for 37 °C for 1 h.
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bioRxiv - Immunology 2020Quote: ... PNGase F (New England Biolabs) digestion was employed to remove N-linked oligosaccharides from Bl-Eng2 ...
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bioRxiv - Microbiology 2023Quote: ... or PNGase F (P0709S NEB) digestion following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μL PNGase F (NEB) and 10 μL water were added into the reaction system ...
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bioRxiv - Immunology 2023Quote: ... PNGase F (NEB, Ipswitch, MA) was used for de-N-glycosylation ...
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bioRxiv - Biochemistry 2023Quote: PNGase F (New England Biolabs) was used to remove N-glycan moieties from proteins ...
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bioRxiv - Biochemistry 2023Quote: ... PNGase F (New England Biolabs) was added to the sample in 1:20 (w/w ...
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bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
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bioRxiv - Molecular Biology 2023Quote: ... is inserted in n-terminal of E2 protein of CHIKV 181/25 structural genes (C-E3-E2-6K-E1) and cloned into pcDNA3.1(+) expression vector using gibson assembly cloning kit (NEB, USA). The resulting plasmid is designated as ChAC-VLP ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... and spike-in lambda DNA (1.25 ng) (New England Biolabs) was sheared using the M220 ultrasound sonicator (Covaris ...
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bioRxiv - Bioengineering 2019Quote: ... coli strain NEB5α (New England Biolabs) for plasmid recircularization and production ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain (C2527I, New England BioLabs). Cultures were grown in 250mL 4xYT-carbenicillin in a 1-liter baffled flask at 37 °C and 300rpm until the OD600 reached between 0.7-0.9 ...
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bioRxiv - Microbiology 2021Quote: ... coli T7 expression strain (NEB 3016). Around 1920 E ...
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bioRxiv - Microbiology 2021Quote: ... coli strain ER2566 (New England Biolabs) containing a pET-24b(+ ...