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Citations for New England Biolabs :
1 - 50 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... N-linked glycans released from glycoproteins using Peptide:N-glycosidase F (PNGase F, New England Biolabs, Ipswich, MA) were mixed with 2,5-dihydroxybenzoic acid (DHB ...
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bioRxiv - Bioengineering 2020Quote: ... reesei strain by peptide N-glycosidase F (PNGase F, NEB, P0704) (Wang et al ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Bioengineering 2019Quote: ... eukaryotic recombinant Stlac2 was denatured with Glycoprotein denaturing buffer at 100 °C for 10 min to be deglycosylated using PNGase F (NEB, USA) according to instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Biochemistry 2021Quote: 20 μg of SARS-CoV-2 Spike trimer was deglycosylated by incubating with 2.5 μL of PNGase F (NEB, SG) under native condition at 37 °C for 4 h ...
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Biochemistry 2020Quote: ... 1 μL of Glycoprotein denaturing buffer (NEB Cat # B0701S, 10X) and 4 μL water were mixed to a total of 10 μL ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were mixed with glycoprotein denaturing buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were sonicated and denatured with Glycoprotein Denaturing Buffer (NEB) by heating them at 100°C for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 μg protein was mixed with 15 μL Glycoprotein Denaturing Buffer (NEB) in 150 μL total volume and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the isolated glycoproteins are treated by α-2,3/6/8 neuraminidase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Cell Biology 2022Quote: ... following the final wash proteins were eluted in Glycoprotein Denaturing Buffer (NEB, P0702S) for 10 mins at 100°C and then resuspended in EndoH (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Peptide-N-glycosidase F (PNGase F) (New England BioLabs) was used to remove all N-linked oligosaccharides for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or for peptide N-glycosidase F (PNGase-F, NEB) digestion using 500 units PNGase-F for 1,5h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... PNGase F digestion (PNGase F, New England Biolabs P0704S) was done per manufacturer’s specifications to remove N-linked glycans ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain (NEB) using ZymoPURE Midi Kit (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... coli strain (NEB) and protein expression was done following the manufacturer directions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... coli strain (NEB) before ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain (NEB). Protein expression was performed using auto-induction protocol42 at 37°C for 24h in 50ml or 100ml culture ...
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bioRxiv - Microbiology 2020Quote: PNGase F (NEB) treatment was performed according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... PNGase F (NEB) treatment was performed on 1μg purified and denatured IGFBP3-flag protein according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... PNGase F (NEB), or DMSO as control for 1 hour (37°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: The standard N-glycoprotein bovine pancreatic ribonuclease B (RNase B, New England Biolabs, USA) was used as a substrate to measure NGLY1 activity ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysate was denatured in glycoprotein-denaturing buffer and digested with PNGase (NEB P0704S) to remove N-linked glycoproteins ...
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bioRxiv - Molecular Biology 2021Quote: ... glycerol-free peptidyl-N-glycosidase F (PNGase F) (New England Biolabs), Endoglycosidase Hf (Endo Hf ...
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bioRxiv - Cell Biology 2022Quote: ... or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, #P0704S) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... coli DH10B strain (NEB #C3019 New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... coli strains (C2987H, NEB) were grown in LB medium at 37°C ...
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bioRxiv - Cell Biology 2020Quote: NEB10β bacterial strain (NEB) was used for the molecular cloning ...
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bioRxiv - Microbiology 2022Quote: ... coli strain ER2566 (NEB) by induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 30°C for 4 h ...
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bioRxiv - Molecular Biology 2022Quote: ... coli DH5α strain (NEB® 5-alpha Competent E ...
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bioRxiv - Genetics 2022Quote: ... coli DH5α strain (NEB® 5-alpha Competent E ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain K12 (NEB Turbo Competent E ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain K12 (NEB Turbo Competent E ...
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bioRxiv - Genomics 2021Quote: ... coli strain (NEB, C3013). 10 ml of overnight cultured E ...
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bioRxiv - Biophysics 2020Quote: ... strain K12 (NEB ER2738) from saturated overnight cultures ...
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bioRxiv - Microbiology 2020Quote: ... coli strain from NEB. A single colony was inoculated into 20 mL of LB and grown at 37 °C with shaking at 150 rpm overnight for primary culture ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain ER2566 (NEB). Overnight pre-cultures were prepared in Luria Broth (LB ...
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Competition co-immunoprecipitation reveals interactors of the chloroplast CPN60 chaperonin machinerybioRxiv - Molecular Biology 2023Quote: ... coli ER2566 strain (NEB) were grown in M9 minimal medium with 15NH4Cl as sole nitrogen source as described earlier (Hammel et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli DH5α strain (NEB), screened by colony PCR ...
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bioRxiv - Cell Biology 2020Quote: ... Glycoprotein denaturation of the cell lysate was performed with 1x glycodenaturing buffer (New England Biolabs) at 95°C for 10 mins ...
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bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
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bioRxiv - Cell Biology 2022Quote: ... or PNGase F (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and PNGase-F (NEB) digestions were performed according to the protocol offered by instruction ...