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Citations for New England Biolabs :
201 - 250 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... as follows: 1µL of 2µM MBTUni-12 primer (5’-ACGCGTGATCAGCRAAAGCAGG-3’) + 1µL 10mM dNTPs Mix (NEB #N0447S) + 8µL Nuclease-free water (Ambion ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse primer Y111E: 5’-TTGATGGAGACATTCTTC-3’) using the Q5 site-directed mutagenesis kit (E0554S, New England Biolabs) to generate a point mutant ...
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bioRxiv - Cancer Biology 2021Quote: ... Universal PCR primers and Index (X) Primer (NEB). PCR products were then purified (AMPure XP system ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli NEB 10-beta (New England Biolabs) was used for plasmid preparation and grown in medium supplemented as instructed by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid was transformed into 10-beta cells (NEB) and clone sequence verified by sanger sequencing.
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bioRxiv - Biochemistry 2022Quote: ... coli NEB 10-beta cells (New England Biolabs) and plated on selective LB agar plates (50 µg/mL kanamycin ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-beta was purchased from NEB (C3020). E ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain NEB 10-beta (New England Biolabs) was used for all cloning ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain NEB 10-beta (NEB Cat# C3019) was used for construction of plasmids by ligation-less Gibson assembly (Fu et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... into 100 µL 10-beta electrocompetent cells (NEB), and then plated on 10 15 cm LBSpect agar plates for 16-20 h (37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 U of beta-agarose I (NEB, M0392), and 5 μL of Maxima H minus reverse transcriptase ...
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bioRxiv - Molecular Biology 2023Quote: ... coli NEB 10-beta cells (New England Biolabs) and all constructs were sequence verified using plasmidsaurus Oxford Nanopore long read sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2023Quote: ... coli strain D10-beta (New England Biolabs Inc.) was used in the entire cloning process ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain NEB10-beta (New England Biolabs #C3019H) grown in LB-medium (Sigma-Aldrich #L3022 ...
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bioRxiv - Microbiology 2024Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2024Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Bioengineering 2024Quote: ... coli NEB 10-beta cells (NEB, cat# C3019H) were used for plasmid construction ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was subsequently performed with primers specific for edited genes and fly sequences using Luna Universal qPCR Mix (New England Biolabs, Ipswich, Massachusetts) and Biorad CFX ConnectTM Real-Time PCR Detection System (BioRad ...
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bioRxiv - Immunology 2021Quote: ... we modified the plasmid using primers EpMap_1 and EpMap_2 along with ssODN EpMap1_ssODN (Extended Data Table 3) using the NEBuilder Mastermix (NEB, E2621S) following the manufacturer’s instructions in order to create hairpin-free overlap regions that could be used for homology-based library cloning ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
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bioRxiv - Systems Biology 2021Quote: ... Adaptors for Illumina sequencing were added via PCR amplification using Nspacer_barseq_pHIMAR (Wetmore et al., 2015) and NEBNext Index 3 Primer for Illumina (New England Biolabs). Cycle conditions were 30 seconds 98°C followed by 4 cycles (15 seconds 98°C ...
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bioRxiv - Molecular Biology 2024Quote: ... using ETAA1 DBR R1 RT-PCR primer 5′-AAGTTCTTCTTCTTGACTTTGTGTT-3′ and treated with RNaseH (New England Biolabs, M0297S). 1μl of the cDNA was used for PCR amplification reactions using using GoTaq® G2 DNA Polymerase (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific mutations in human PSEN1 were obtained by Q5 site-directed mutagenesis of the pMSCV-puro vector containing wild type human PSEN1.26 We designed primers for Q5 site-directed mutagenesis (New England Biolabs) with NEBaseChanger.neb.com and performed site-directed mutagenesis according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and qPCR performed (NEB Luna qPCR MasterMix) on ald and rp49 (aldF2 ...
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bioRxiv - Molecular Biology 2022Quote: ... using the random primer method (Random Primer 6, NEB S1230S ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E. coli, NEB, Ref.C3020K) and grown overnight on LB plates supplemented with 50 μg/mL Carbenicillin (Carbenicillin disodium salt ...
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bioRxiv - Physiology 2023Quote: ... 300nmol-L of a gene specific primer (thermofisher scientific, USA) and 10uL of Syber green qPCR mastermix (New England Biolabs, Ipswich, Massachusetts, USA) each used as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA isolated from Human Bronchial Epithelial Cells was reverse transcribed using oligo dT primers and ProtoScript II (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Enhancers were cloned from human or C57Bl/6J mouse genomic DNA using enhancer-specific primers and Phusion high-fidelity polymerase (M0530S; NEB). Individual enhancers were then inserted into a recombinant single-stranded pAAV backbone that contained the beta-globin minimal promoter ...
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bioRxiv - Systems Biology 2021Quote: ... 3 μL of the reverse phasing primer pool (100 nM) and 15 μL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Bioengineering 2023Quote: ... and assembled with PCR amplified (primers 2 and 3) pRSET vector fragment using Gibson assembly (NEB Japan, Tokyo, Japan) to add T7 promoter ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1/2/3/4) following the manufacturer’s instructions (New England Biolabs). Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL of the reverse phasing primer pool (100 nM) and 15 µL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Genomics 2024Quote: M13seq primer (5’-GACGTTGTAAAACGACGGC-3’) was labeled with 32P at 5’-end by T4 polynucleotide kinase (New England Biolabs). Primer/template (p/t ...
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bioRxiv - Cell Biology 2021Quote: ... Universal PCR primers and Index (X) Primer (New England BioLabs). At last ...
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bioRxiv - Genomics 2020Quote: ... the plugs were digested with beta-agarase (NEB M0392) as described by the manufacturer to liberate DNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-Beta electrocompetent (New England Biolabs, MA, USA) or in-lab made equivalent for increased transformation efficiency ...
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bioRxiv - Microbiology 2021Quote: ... coli DH10B (New England Biolabs 10-beta, cat#C3020K), selected on LB supplemented with 50 μg/ml kanamycin (LB+KA50) ...
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bioRxiv - Genetics 2020Quote: ... coli competent cells (10-beta Competent E.coli, NEB C3019I) through an incubation of 30 minutes on ice and a heat shock of 30 seconds at 42°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... NEB 10-beta cells (New England Biolabs, Ipswitch, MA) were used for cloning and testing of all constructs ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 10-beta from New England Biolabs (NEB) was used for sensor assays ...
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bioRxiv - Cell Biology 2024Quote: ... Assemblies were transformed into 10-beta competent cells (NEB) and plated onto LB agar plates for overnight incubation at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... transformed into NEB 10-beta electrocompetent cells (NEB C3020K), and extracted using the Qiagen Plasmid Maxi Kit (Qiagen 12162) ...
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bioRxiv - Synthetic Biology 2024Quote: ... or electroporation (NEB 10-beta, Cat# C3019H, 1.8 kV) with 50 ng of plasmid DNA ...
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bioRxiv - Cell Biology 2021Quote: ... 80ng of genomic DNA were fragmented to around 170bp followed by End Pair and A tailing with the End Pair mix (NEB Next Ultra DNA library preparation kit). After ligation of truncated TruSeq adapters with the NEB Next Ultra Ligation Module ...