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Citations for New England Biolabs :
1 - 50 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 1 µL of the boiled colony was used for PCR with primer pair (JGI_27F: 5’-AGAGTTTGATCCTGGCTCAG-3’and JGI_1391R: 5’-GACGGGCRGTGWGTRCA-3’) with NEB Q5 Polymerase (New England Biolabs, Ipswitch, MA). The PCR amplicon was confirmed by agarose gel electrophoresis and the sequence was determined using conventional Sanger Sequencing (Genewiz LLC ...
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bioRxiv - Microbiology 2021Quote: ... were generated by PCR amplification of either full-length pfget4 or nucleotides encoding for the first 246 amino acids of PfGet2CD using PfGet4-EcoRIF (5’-GTACCGGAATTCATGAAAAAGTTCAAATTTAGTAAAGAAAAGCTAGCC-3’)/PfGet4-XhoISalIR or PfGet2CD-BamHIF (5’-GTACGCGGATCCATGGATAAAAATACATTAAAAAGAA-3’)/PfGet2CD-XhoISalIR (5’-AGACCGGTCGACCTCGAGTTATTCATGTTTCGTAATAATAAATTG-3’) primer pairs and subsequently cloning at corresponding sites in pMALc2X plasmid (New England Biolabs).
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bioRxiv - Cancer Biology 2023Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single-cell libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single -cell libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... A different Unique Dual Index Primer Pair (NEB, E6440S) was added to each sample and 11 cycles were used for the PCR enrichment ...
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bioRxiv - Synthetic Biology 2021Quote: ... qPCR was carried out using the primers in Supplementary Table 3 and Luna Universal qPCR Master Mix (NEB) in a BioRad iCycler in technical triplicates for each biological replicate ...
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the clpP2 gene were amplified by PCR using the A–B and C–D primer pairs from table 3 (Phusion polymerase, GC buffer, New England Biolabs). The PCR products were purified with E.Z.N.A ...
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bioRxiv - Plant Biology 2021Quote: ... Flanking sequences 5’ and 3’ of the coding regions were amplified with appropriate primer pairs (Table S2) using Phusion DNA polymerase (New England Biolabs) and cloned into pDONR 221 P1-P4 and pDONR 221 P3-P2 ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed using specific reaction primer pairs specific to the appropriate parental segment (Table 3) and Q5 High-Fidelity Polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: RNA-sequencing libraries were prepared by depleting eukaryotic ribosomal RNA with the NEBNext rRNA Depletion Kit prior to library synthesis with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and addition of multiplex oligos using the Unique Dual Index Primer Pairs Set 3 (NEB). Library quality control was performed using Qubit and Bioanalyzer 2100 ...
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bioRxiv - Microbiology 2022Quote: ... Backbone was amplified with primer pair 3/4 and fragments were assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... was PCR amplified with mutagenic primer pairs using Q5 polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs) (E6440, NEB). We used 13 min fragmentation time and 9 cycles of PCR enrichment on 1000 ng of total RNA input determined by preliminary library preparation ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR conditions were followed according to each primer pair as recommended by NEB using pCMV-SMAUG1-HisTEV2xFLAG as the template ...
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bioRxiv - Genetics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB). Sequencing was performed using a SP Reagent Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Index Primers Set 3 (NEB). Amplified libraries were cleaned up using Sample Purification Beads (NEB ...
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bioRxiv - Immunology 2023Quote: ... PCR was then performed on genomic DNA with relevant primer pair and enzyme (NEB) (see materials and methods chapter for PCR programme) ...
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bioRxiv - Neuroscience 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001) ...
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bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S). Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche #08963851001) ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were indexed and amplified with NEBNext UDI primer pairs (New England Biolabs, #E6440S) during 8 PCR cycles with NEBNext HiFi PCR Master mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #6442S/L). Sequencing of the libraries was performed with Illumina NovaSeq 6000 SP flow cell using paired-end 150-bp format (300 cycles = 2×150bp ...
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bioRxiv - Plant Biology 2022Quote: ... Four vectors including one of the active 5′gRNA-pairs and one of the active 3′gRNA-pairs were digested by BglI (New England Biolabs) and ligated at once ...
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bioRxiv - Immunology 2021Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Immunology 2020Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... each primer pair was annealed and consequently phosphorylated with T4-polynucleotide kinase (T4-PNK; NEB). Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs) were used for creating a genomic DNA library ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... was combined with forward and reverse primers (Supplementary Table 3) and the Luna Universal qPCR Master Mix master mix (M3003, NEB) containing SYBR green and ROX passive dye to a final 10ul reaction volume ...
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bioRxiv - Cancer Biology 2020Quote: ... Illumina compatible NEBNext Multiplex Dual Index Primer pairs and Adaptors (New England Biolabs, Cat. No. E6440S) were then ligated to the ends of the size-selected chimeric fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina-compatible NEBNext Multiplex Dual Index primer pairs and adapters (New England Biolabs, cat. no. E6440S) were added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs E6440S) and polyA enrichment module (NEB E7490S) ...
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bioRxiv - Genetics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4) (NEB, E6446S) as index primers ...
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bioRxiv - Cancer Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) as per manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: ... in conjunction with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440S), according to the manufacturer’s instructions ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs #E64460S) and polyA enrichment module (NEB #E7490S) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Level 0 plasmid was amplified with the primer pair SLo0765/SLo0766 followed by DpnI (NEB) digestion prior to ligation (any Level 0 plasmid will do ...
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bioRxiv - Immunology 2021Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...
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bioRxiv - Genomics 2023Quote: ... with NEBNext Multiplex Oligos (96 Unique Dual Index Primer Pairs) for Illumina Barcodes (New England Biolabs, USA) and sequenced on an Illumina NovaSeq 6000 S2 150bp PE flow cell at the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies Core.
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bioRxiv - Microbiology 2023Quote: ... in combination with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs; New England BioLabs), following the manufacturers’ recommended protocols ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Cell Biology 2024Quote: ... using NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs (#6440S, New England Biolabs, US). All generated cDNA libraries were amplified with 7 cycles of final PCR.
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bioRxiv - Developmental Biology 2024Quote: ... 8 PCR cycles for enrichment of adaptor-ligated DNA with unique dual index primer pairs from NEB. The libraries were sequenced on the NovaSeq 6000 system with NovogeneAIT Genomics ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB #E6440). Quality control of the libraries was done with the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB) to introduce point mutations for the T161V and D296N substitutions ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye ...