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Citations for New England Biolabs :
1 - 50 of 5823 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... 25μL NEB Next HiFi PCR Mix (2x solution, NEB M0544), 10μl H2O) ...
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bioRxiv - Biophysics 2024Quote: ... Molecular Biology Grade” (NEB) and incubated for 5 min at 3°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Molecular Biology Grade (NEB), or Recombinant Albumin ...
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bioRxiv - Synthetic Biology 2023Quote: ... Molecular Biology Grade (NEB), diluted to 10 mg/mL.
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bioRxiv - Genomics 2020Quote: ... 50mM NaCl) with added 5M NaCl to 288mM NaCl Final combined with 5uL Proteinase K (NEB).
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bioRxiv - Genetics 2022Quote: NGS libraries were generated by amplifying 12 μl of the eluted CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (90) (Suppl. Table 4) with NEBNextHiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sgRNA containing sequences of the genomic template DNA were amplified by PCR using the indicated Primers of HPLC grade (S2) and Q5 Hot Start high-fidelity DNA polymerase (New England BioLabs). The PCR products of the toxin resistant cells and untreated library cells were analyzed by deep sequencing using an Illumina-based procedure via a commercial supplier (Eurofins Genomics).
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bioRxiv - Genomics 2021Quote: NGS libraries were generated by amplifying the CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (Buenrostro et al., 2015) (Supplementary Table 6) with NEBNext® HiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
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bioRxiv - Plant Biology 2022Quote: ... molecular biology grade (New England Biolabs, B9000S). dNTPs (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.01% Molecular Grade BSA (New England Biolabs), 146 mM NaCl (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison, WI; Trypsin-ultraTM, MS grade NEB) with a final trypsin concentration of 20 ng/µl in 50 mM aqueous ammonium bicarbonate and 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µL PCR Anchor Primer,1 µL Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, Inc., N0447L), 10 µL Q5® Reaction Buffer (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Genomics 2024Quote: ... NaCl 5M stock was added to reach 1 M final concentration along with 30 µl of Streptavidin magnetic beads (NEB # S1420S) for 2 hrs with end-over-end mixing at 4°C to capture biotinylated DNA fragments ...
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bioRxiv - Molecular Biology 2023Quote: C2566 competent cells (cloning grade) were provided by NEB. ER2948 competent cells were prepared by a modified rubidium chloride method ...
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bioRxiv - Biophysics 2022Quote: ... Total of 60 ug of trypsin (mass spectrometry grade, NEB) was diluted in 25 mM NH4HCO3 and added to dried gel pieces (3x volume of the gel volume) ...
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bioRxiv - Microbiology 2023Quote: ... Amplified PCR products were ran on 2% agarose gel and stained with ethidium bromide solution (New England Biolabs Inc.). Gel photographs were taken using ChemiDoc MP gel documentation system (BIO-RAD).
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bioRxiv - Cell Biology 2023Quote: ... Samples were treated with 20µl of ProtK (NEB Molecular Biology Grade), incubated for 3 hrs at 55°C and 5 hrs at 65°C to reverse crosslinks ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.45% Tween-20) and Proteinase K (NEB, molecular biology grade) diluted tenfold in NEB storage buffer (20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... The solution was directly used as a template for PCR amplification using Q5® High-Fidelity 2X Master Mix (NEB) (primer pair ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% BSA (molecular grade BSA from Biolabs or Acetylated BSA from Ambion), and Tween-20 at 0.1% ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of the heated solution was then used as template for PCR using the Q5® High-Fidelity DNA Polymerase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 5 uL of gDNA solution was used as template in the 50 uL PCR reaction using Q5 High-Fidelity 2X Master Mix (NEB, M0492) with the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
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bioRxiv - Cancer Biology 2021Quote: ... Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml) ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μL of molecular biology grade bovine serum albumin (20 mg/mL, New England Biolabs), 5 μL of 5× Phire Buffer ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were PCR purified using Monarch® PCR DNA Cleanup Kit (New England BioLabs) and eluted with water ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Genomics 2021Quote: ... Post-PCR each PCR aliquot was exonuclease I (NEB) treated ...
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bioRxiv - Biochemistry 2023Quote: ... and amplified by PCR using Pfusion PCR polymerase (NEB) with specific primers or random hexamer primers ...
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bioRxiv - Biochemistry 2021Quote: ... rinsed with PBS and incubated in PBS containing 3% molecular biology grade BSA (New England Biolabs) and 0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 0.3 μL of 1 mg mL−1 trypsin (trypsin-ultra, MS-grade, New England Biolabs) was added and samples were incubated at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.2 µL solution A (NEB), 1 µL factor mix (NEB) ...
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bioRxiv - Cell Biology 2024Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... PCR clean-up (Monarch PCR and DNA Cleanup Kit, NEB) and gel extraction (Monarch Gel Extraction Kit ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were purified (Monarch PCR Cleanup Kit (NEB, #T1030S)) and restriction digested with unique 5’ SpeI and 3’ PspXI sites ...
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bioRxiv - Microbiology 2020Quote: ... PCR was done using LongAmp® Taq PCR Kit (NEB) from 40 ng of QMA0248 S ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Synthetic Biology 2024Quote: ... PCR were performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR and genotyping (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR was performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... that had been blocked and equilibrated into the binding buffer supplemented with 0.1% Tween 20 and 0.1μg/μl of BSA (Molecular Biology Grade, NEB). Protein-RNA complexes were then incubated with the magnetic beads on a shaker for further two hours ...
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bioRxiv - Biochemistry 2022Quote: ... PNGase F (P0704) and mass spectrometry grade Trypsin (Trypsin-Ultra, P8101) are from New England Biolabs (NEB). Fbs1-GYR proteins were expressed and purified from E ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Molecular Biology 2023Quote: All restriction enzymes were purchased from NEB and PCR amplification was performed with Q5 High-Fidelity PCR (NEB) following the manufacturer’s recommendations ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... were PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB) and cloned into pCFD4 (Addgene 49411 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (NEB) before GGA assembly ...