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Citations for New England Biolabs :
2401 - 2416 of 2416 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... A separate PCR reaction was performed for each position using the pJet-MYH7-mTagBFP2-EF1α-BSD plasmid backbone as template with Q5 polymerase (NEB; cat. no. M0491S) and 5 μM bespoke forward and reverse primers (IDT) ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were inserted at the leu1 locus after linearization with the restriction enzymes NdeI or NruI (New England Biolabs #R0111S and #R0192, repectively). Positive clones were selected by auxotrophy selection and nourseothricin or G418 resistance ...
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bioRxiv - Biochemistry 2023Quote: ... PCR fragments were cloned into plasmid via Golden Gate with BsaI-HF v2 or Gibson assembly (New England Biolabs, Gibson Assembly Protocol (E5510)) ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were either amplified from available plasmids or synthesized gBlocks™ (Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (New England Biolabs, Cat. #M0494S). The generated plasmids were transformed into Zymo JM109 chemically competent E ...
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bioRxiv - Cancer Biology 2024Quote: ... and pLARRY-EGFP with primers homologous to the vector insertion site in a custom synthetic lentiviral plasmid backbone (Vectorbuilder, Inc) using Gibson assembly (Gibson Assembly® Master Mix, NEB, Ref. E2611L). For recombinase lentivirus libraries ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Biochemistry 2021Quote: ... MODY3-associated variants (P112L, R263C, N266S) were introduced into (DD-)DBD pTH27 expression plasmids by Q5® site-directed mutagenesis (New England Biolabs, Ipswich, MA, USA). These constructs were used for recombinant protein expression and purification ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNA purity and concentrations were determined by UV260/280 spectrophotometry and gel electrophoresis via plasmid digestions using the digestion enzymes EcoRI and XhoI (New England Biolabs # R3101S and R0146S respectively).
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bioRxiv - Molecular Biology 2022Quote: ... A CEN plasmid harbouring a full length HAC1 is used as template and Q5® High-Fidelity DNA Polymerase (New England Biolabs Inc., MA, USA) is used ...
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bioRxiv - Genetics 2023Quote: ... The oligonucleotides were annealed and subcloned into the Csy4-spCas9 vector plasmid (pCas9Pvcsg) using the Golden Gate reaction with restriction endonuclease BsaI-HF v2 (New England BioLabs, Ipswich, MA, catalog #R3733L). The expression of Csy4 ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into plasmid pKNG101 by using the NEBuilder® HiFi DNA Assembly Cloning kit (New England Biolabs, Ipswich, MA, USA) (33) ...
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bioRxiv - Bioengineering 2023Quote: ACE2 plasmids with different cytoplasmic tail lengths were generated using the Q5® Site-Directed Mutagenesis (SDM) Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The barcoded and mapped sequence variants were then cut out of the plasmid backbone using PasI and ligated into PasI-digested and dephosphorylated λ DNA (λSam7/cI857, NEB #N3011L, Ipswitch, MA, USA). This strain background is obligately lytic at 37°C and has an amber-suppressible lysis system that can infect and kill E ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-2 signal sequence that was present in the original mCherry-progranulin plasmid was later replaced with progranulin signal sequence by site-directed mutagenesis (NEB Q5 site directed mutagenesis kit). For cloning of Prosaposin-RUSH (Str-KDEL_Prosaposin-SBP-mCherry) ...
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bioRxiv - Microbiology 2024Quote: ... were amplified using the primers listed in Table S2 and cloned into the expression plasmids (van der Kolk et al., 2020) with NcoI and XhoI (New England Biolabs GmbH, Frankfurt am Main, Germany). Correct plasmids were methylated by transforming them to ER1821 containing pM.EsaBC4I ...