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Citations for New England Biolabs :
1701 - 1750 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... coli cell libraries using M13K07 helper phage (New England Biolabs, Whitby, Canada, Cat#N0315S) as described in 81 and used for selection experiments described below.
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bioRxiv - Microbiology 2021Quote: ... coli JP313 ΔtolC strains using a Q5 high-fidelity DNA PCR (New England Biolabs). The primers used for these reactions were “EM029-TMKF1” (5’-ATGGCAAACTTTCTCGTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The Escherichia coli strain used for routine cloning was NEB 5α (New England Biolabs). The plasmids used in this article are listed below (Table S2) ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were amplified by transformation into chemically competent Escherichia coli DH5α (New England Biolabs), which was plated onto LB agar (10 g/L tryptone ...
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bioRxiv - Molecular Biology 2021Quote: ... The subcloned AGO plasmids were transformed into Escherichia coli BL21 (DE3) (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: All cell extracts for CFE were prepared with Escherichia coli BL21 Star(DE3) (NEB).21 These cells were grown ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21(DE3) and purified by affinity chromatography using amylose resin (New England Biolabs). Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel) ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain using the Monarch® Plasmid Miniprep Kit (NEB, Ipswich, MA, USA). The extracted plasmid was linearized with restriction enzyme BamHI (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... coli PURExpress system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were recovered by transformation of Escherichia coli NEB5α F’IQ (New England Biolabs) and selected on LB agar supplemented with 50 μg/ml Kanamycin ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... coli following the NEBuilder HiFi DNA Assembly protocol (New England Biolabs; Ipswich, United States). Knockouts of A ...
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bioRxiv - Genomics 2023Quote: ... and second-strand cDNA using Escherichia coli DNA ligase and polymerase (New England Biolabs), followed by a clean up step (1.8:1 beads to sample ratio) ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cells for expression following the manufacturer’s instructions (New England Biolabs). Colonies harbouring pET-rHl ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: The Gibson products are transformed into T7 express competent Escherichia coli cells (NEB C2566). Individual colonies are grown in 600 μL Luria Broth (RPI L24400 ...
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bioRxiv - Biochemistry 2023Quote: ... were transformed with Lpg2248 (LotA) plasmids and T7 express Escherichia coli competent cells (NEB) were transformed with Lpg1621 (LotB ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Exonuclease III to generate ssDNA pools according to manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells using the Q5 mutagenesis kit in accordance with the manufacturer’s instructions (NEB). For FACS rescue experiments with WT-CHC22 or 11TD-CHC22 ...
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bioRxiv - Synthetic Biology 2024Quote: ... integration vector backbones were transformed into chemically competent NEB Stable Escherichia coli (NEB #C3040H), and cells were grown at 30 °C ...
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bioRxiv - Microbiology 2021Quote: ... in the 5’end of 30 mer RNA substrate we used the vaccinia virus capping enzyme following the manufacturer’s protocol (New England Biolabs), except that the methyl donor SAM was absent and 0.05 units of inorganic pyrophosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2 -O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Evolutionary Biology 2020Quote: Purified DNA library (250 ng) was cut with the Hind III and Bam HI restriction enzymes (NEB) for 1h at 37 °C in a reaction mixture containing 1X of the NEB cut smart buffer ...
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Genomics 2020Quote: ... We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly) using 125ng of gene DNA ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Genetics 2019Quote: ... 2012) by removing cbr-Pmyo-2∷gfp∷his-72 UTR by cutting using KpnI and ApaI (NEB). The digested pZZ0031 backbone carrying cbr-unc-119(+ ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed in the pUC19 backbone using NEBuilder Hi-Fi DNA Assembly Mastermix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA (28) using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (sTable 5) ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Cell Biology 2019Quote: Cells transfected with corresponding SNAP-tags and stained with silicone-rhodamine dye (SNAP-cell 647-SiR (NEB) (3 µM) ...
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bioRxiv - Cell Biology 2019Quote: Cells transfected with the SNAP-tag and stained with low concentrations of SNAP-cell 647-SiR (NEB) (15 nM for MIC60-SNAP and 0.225 nM for MIC10-SNAP ...
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bioRxiv - Cell Biology 2020Quote: ... a P2A self-cleavage site and a CAAX membrane localization tag were added using Q5 mutagenesis (NEB) with the following primers ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... in frame with the N-terminal His6-tag using the Gibson assembly master mix (New England Biolabs) for TaSS4-1B ...
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bioRxiv - Biochemistry 2021Quote: The full-length PARP1 gene was purchased from GE Healthcare and subcloned into a pACEBac1 plasmid bearing an N-terminal 6xHis-tag via a Gibson Assembly (NEB). The PARP2 expression plasmid (C-terminal FLAG-6xHis-tag ...
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bioRxiv - Cell Biology 2021Quote: ... SNAP tag was firstly PCR amplified from pSNAPf Vector (Cat. #N9183S, New England Biolabs, Ipswich, MA, USA) using primers 5’-CGT ACG ATC GAA TTC CCC ATG GAC AAA GAC TGC GAA ATG AAG CGC ACC ACC-3’ (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... with a cleavable Glutathione-S-Transferase (GST) tag at the N-terminus) and pMALTMc5X (New England Biolabs, USA ...
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bioRxiv - Plant Biology 2022Quote: ... and 3xHA::3C::BLRP tag encoding DNAs were respectively ligated into pENTR/D constructs using AscI (NEB) digestion site in the pENTR/D vector ...
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bioRxiv - Cell Biology 2023Quote: cDNA encoding recombinant MUC17(7TR) with N-terminal 3xFlag tag was generated using Gibson Assembly (E2611S, NEB) following the manufactures protocol ...
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bioRxiv - Microbiology 2023Quote: ... fused to a maltose-binding tag using the pMAL™c5X-vector (New England Biolabs, Ipswich, US). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO21-92 and ISG1579-156 without tag and subsequently cloned into the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA with retrotranscriptase from Moloney murine leukemia virus (Ref. M0253, New England BioLabs) and random hexanucleotides as primers by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE, NEB) in the presence of GTP and S-adenosylmethionine (SAM) ...
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bioRxiv - Biochemistry 2020Quote: ... a maltose binding protein (MBP) and a Tobacco Etch Virus (TEV) protease cleavage site in the N-terminal via Gibson assembly (New England Biolabs) as per the manufacturer’s protocol.