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Citations for New England Biolabs :
1651 - 1700 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with SNAP-tag ligand SNAP-Cell 647-SiR (New England BioLabs; S9102S) at a final concentration of 100 nM for 15 minutes followed by 30-45 minute washout in i3Neuron Maintenance Media before imaging in i3Neuron Imaging Media ...
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bioRxiv - Plant Biology 2023Quote: AtPAXX fused to a hexahistidine tag (pHAT4-atpaxx) was expressed in BL21(DE3) cells (NEB). The proteins were purified using Ni-NTA (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences.
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bioRxiv - Cell Biology 2023Quote: ... and an internal ribosomal entry site driven SNAP-tag (N9181S, New England BioLabs, Ipswich, MA), respectively ...
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Biophysics 2021Quote: ... coli DNA was performed identically using Nb.BsrDI and 1X CutSmart (New England Biolabs) substituted for the two enzymes used to prepare λ-DNA ...
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bioRxiv - Biochemistry 2021Quote: Plasmid propagation and subcloning was performed using Escherichia coli DH10β competent cells (NEB). The codon optimized GTL gBlock (Supporting Information Table 1 ...
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bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... coli SHuffle T7 Express lysY and the restriction enzymes were purchased from NEB Japan ...
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bioRxiv - Plant Biology 2021Quote: ... constructs were separately introduced into the Escherichia coli (Express Competent cells C2523H, NEB). The purification was performed using amylose resin (NEB ...
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bioRxiv - Bioengineering 2021Quote: This study used the Escherichia coli strains NEB10β (New England Biolabs, Ipswich, MA), Nissle 1917 as well as all strains listed in Table 1 ...
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bioRxiv - Biochemistry 2019Quote: NHERF1 PDZ2-DTRL was expressed in Escherichia coli BL21 (DE3) (New England BioLabs). Cultures in auto-induction media (12 g/L tryptone ...
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bioRxiv - Molecular Biology 2022Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells following the recommended High Efficiency Transformation Protocol (C2987, New England Biolabs) described by the commercial strain provider ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (Table S3) were propagated in Escherichia coli DH5α cells (NEB, United Kingdom) by growing cells in LB media without NaCl (Formedium ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were subjected to high fidelity Q5 Polymerase (NEB, Singapore; Cat: M0491L) PCR to generate 200 bp templates of the Ag43 signal peptide (Figure 1A) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli and grown at 30°C to ensure construct stability (New England Biolabs). Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Total RNA samples were polyadenylated using Escherichia coli poly(A) polymerase (NEB, M0276L) for 5 min and purified by GeneJET RNA Cleanup and Concentration Micro Kit (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... coli (MG1655) genome containing the rrnC operon was amplified by PCR (Phusion, NEB) using the following primers:
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bioRxiv - Microbiology 2023Quote: ... coli (High Efficiency) (Cat. No. C3019H, New England BioLabs, Inc, Ipswich, MA, USA) following the manufacturer’s instructions with a recovery time of either 1 hour (standard time ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Both HURP plasmids were transformed into Rosetta2 (DE3) Escherichia coli cells (NEB: C29871) and grown in 2 L of LB media containing 50 μg/mL ampicillin and 25 μg/mL chloramphenicol at 37 °C in a shaking incubator (200 rpm) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, catalog no. C3013) and purified as previously described ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... coli DH5alpha colonies using OneTaq® 2X Master Mix (New England Biolabs, USA) to confirm the correct insertions and further sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli were performed with the Monarch® Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
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bioRxiv - Genomics 2023Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrations measured using Qubit 1X dsDNA HS Assay) was treated with 5U of RNAse HI (NEB) in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... ENTR and DEST vectors were generated using Gibson assembly (NEB Builder Hi-Fi DNA assembly #E2621S) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of His-PKR was dephosphorylated using 3,200 units of λ-PPase (New England Biolabs) in 200 μl reaction buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... the refolded 6×His-tagged precursors were treated with enterokinase (New England Biolabs, Ipswich, MA, USA) to remove their N-terminal 6×His-tag according to our previous procedure [3,18] and purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18 ...
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bioRxiv - Neuroscience 2023Quote: Plasmids for the trans-Tango components were generated using the Hi-Fi DNA Assembly (NEB #E5520S), BP Gateway Cloning (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Hi-C single-index library preparation was performed as previously described56 using MboI (New England Biolabs) restriction enzyme.
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bioRxiv - Microbiology 2023Quote: ... 750-basepair regions flanking each gene were amplified by PCR and Hi-Fi DNA Assembly (NEB) was used to clone into pExchange-tdk ...
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... together with an N-terminal FLAG-emGFP-tag via NheI and NotI (both New England Biolabs) restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... the 6xHis-MBP tag was cleaved from 6xHis-MBP-MISP using a TEV protease (NEB; P8112) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
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bioRxiv - Biochemistry 2023Quote: ... the Myc/DDK tag was removed via the Q5 site-directed mutagenesis kit (New England Biolabs) using the oligonucleotides mS29 ΔMyc-DDK Forward ...
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bioRxiv - Plant Biology 2024Quote: ... Mixed tissue sections and reaction reagents: 2 μl 10× Tag DNA polymerase (NEB, Cat. No. M0267V), 0.4 μL Buffer Tag DNA polymerase (5 U/μL ...
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bioRxiv - Biophysics 2020Quote: ... was cloned into pET28a and expressed in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Bioengineering 2022Quote: ... Sequence verified plasmids were transformed into BL21(DE3) Escherichia coli cells (New England Biolabs) and plated on LB medium supplemented with 100 μg mL−1 ampicillin ...
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bioRxiv - Synthetic Biology 2019Quote: The bacterial strains used in this study includes Escherichia coli DH5α (New England Biolabs), Escherichia coli Nissle 1917 (isolated from probiotic Mutaflor capsule) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid cloning and multiplication were performed in Escherichia coli DH5α (New England Biolabs, USA). E ...