Labshake search
Citations for New England Biolabs :
1501 - 1550 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
-
bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
-
bioRxiv - Microbiology 2022Quote: ... and then ligated by Hi-Fi DNA assembly (New England Biolabs Inc.). Irgb6-WT and Irgb6-T95D were expressed and purified as described (Saijo-Hamano et ...
-
bioRxiv - Biochemistry 2023Quote: ... was cloned into pUC19 by Gibson cloning (Hi-Fi cloning system, NEB) to replace the yeast sequence:
-
bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
-
bioRxiv - Microbiology 2022Quote: ... The virus lysate was then denatured by boiling in denaturing buffer (New England BioLabs) for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs ...
-
bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
-
bioRxiv - Bioengineering 2020Quote: ... coli DH10B strain (NEB #C3019 New England Biolabs, Ipswich, MA) using PureLink Quick Plasmid Miniprep Kit (Invitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... for HlgA and in BL21 (DE3) Escherichia coli cells (NEB) for HlgB ...
-
bioRxiv - Cell Biology 2020Quote: KCM competent bacteria (Escherichia coli strain K12 CJ236, NEB, E4141) were transformed with 3Cs multiplex template plasmid template by mixing 100 ng of DNA with 2 µl of 5x KCM buffer (0.5M KCl ...
-
bioRxiv - Microbiology 2019Quote: ... coli strains NEB 5-alpha F’lq (New England Biolabs, US) and MC1061 with plasmid pKD46 which carries ARA-inducible Red recombination system24 ...
-
bioRxiv - Neuroscience 2020Quote: ... coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
-
bioRxiv - Neuroscience 2020Quote: ... coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 0.2 % glucose and 100 mg/L ampicillin until OD600 reached 0.3-0.37 ...
-
bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) cells (New England Biolabs, Ipswich, MA, USA) were transformed with the untagged SiRHP-expressing pBAD vector (Askenasy et al. ...
-
bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
-
bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) (New England Biolabs, Frankfurt am Main, Germany) cells were transformed with the pET22b-BcnrdI plasmid ...
-
bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
-
bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
-
bioRxiv - Cell Biology 2022Quote: ... coli BL21(DE3) cells (New England Biolabs, cat. no. C2527I). A starter culture grown in LB broth (Sigma ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli Poly(A) Polymerase (10 units; M0276S, New England Biolabs) with nuclease-free water (29 μL) ...
-
bioRxiv - Microbiology 2021Quote: ... coli BL21(DE3) cells (T7 Expression strain, New England Biolabs), expressed from a pET28b plasmid ...
-
bioRxiv - Microbiology 2021Quote: ... All assays were done in Escherichia coli (strain: NEB Turbo) following the same plasmid protection assay described previously ...
-
bioRxiv - Microbiology 2019Quote: ... All pCas9 constructs were transformed into Escherichia coli (NEB Turbo) by heat-shock and made electrocompetent as described (protocols.io ...
-
bioRxiv - Microbiology 2020Quote: ... coli cloning strains such as NEB-5a (New England Biolabs) or BH10c,48 with antibiotics added at the following concentrations ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli Stbl competent cells (New England Biolabs, Ipswich, MA, USA), colonies were screened by PCR ...
-
bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
-
bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs #C3020K), and bottlenecked to ∼1 × 105 cfus (an average of >25 barcodes per single-mutant) ...
-
bioRxiv - Biochemistry 2022Quote: ... coli were transfected using the High-Efficiency Transformation Protocol (NEB). Single colonies were selected from carbenicillin LB plates and plated into 5 mL starter cultures ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
-
bioRxiv - Microbiology 2022Quote: Escherichia coli strain NEB Stable (New England Biolabs, Ipswich, MA), Pseudomonas aeruginosa strains PA01 and PA14 (generous gift from Dr ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Coli BL21(DE3) strain (purchased from New England Biolab, NEB), BL21Marioentte (purchased from Addgene ...
-
bioRxiv - Biochemistry 2023Quote: ... coli strain SHuffle T7 (New England Biolabs Inc., Massachusetts, USA) and plated on LB agar containing 30 μg/mL kanamycin and 2% glucose ...
-
bioRxiv - Bioengineering 2023Quote: ... coli MG1655 gDNA (cat no. T3010, New England Biolabs Inc.) via PCR ...
-
bioRxiv - Microbiology 2023Quote: ... Strains used were: Escherichia coli T7 Shuffle (New England Biolabs) to express Rv0365c ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21 strain resistant to phage T1 (New England Biolabs) harboring the pRARE2 plasmid and plated onto LB+Agar plates supplemented with kanamycin (Kan ...
-
bioRxiv - Genetics 2024Quote: ... coli competent cells (New England Biolabs, MA, USA, Cat # C2987H) from the pDONR 221 entry vector to the pGW-HA.attB destination vector using Gateway cloning and the sequences were confirmed by Sanger sequencing ...
-
bioRxiv - Microbiology 2024Quote: ... Competent strains of Escherichia coli DH5-α (New England Biolabs), used for DNA cloning and plasmid propagation ...
-
bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
-
bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
-
bioRxiv - Biophysics 2024Quote: ... and then transformed BL21(DE3) Escherichia coli (New England BioLabs) with this plasmid ...
-
bioRxiv - Biochemistry 2020Quote: ... Tag introduction and mutagenesis was achieved through Gibson assembly (New England Biolabs) to generate SMC1-His ...
-
bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
-
bioRxiv - Biophysics 2021Quote: ... and the His6-MBP tag was subsequently recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
-
bioRxiv - Cell Biology 2023Quote: ... and subsequently the 6xHIS tag was cleaved using the TEV Protease (NEB) by adding TEV enzyme assuming all protein isolate was 6xHIS tagged based on the activity of the TEV protease ...
-
bioRxiv - Biochemistry 2023Quote: ... and SNAP tags were stained with SNAP-Cell® 505-Star (NEB). HaloTag® Ligands TMRDirect were added to medium to a final concentration of 50nM and incubated overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... The 3XFLAG tag was replaced by Q5® site-directed mutagenesis (NEB) with either a Myc-tag or Strep-tag®II ...
-
bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: Virus stocks were generated by either transfecting purified in vitro transcribed viral RNA (HiScribe, NEB) or transfecting the infectious cDNA clone containing plasmid in BHK-T7 cells ...
-
bioRxiv - Biochemistry 2023Quote: Infection in HCT116: Cells were infected with virus and 8 μg/mL polybrene (NEB, #H9268).