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Citations for New England Biolabs :
1551 - 1600 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing the SNAP-tag and stained with SNAP-cell 647-SiR (NEB) were excited with a white light laser at an excitation wavelength of 633 nm ...
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bioRxiv - Cell Biology 2019Quote: ... the sequence encoding SNAP-tag was PCR amplified from pSNAPf (New England BioLabs) with primers to append flanking BamHI and NotI restriction sites (Forward ...
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bioRxiv - Biophysics 2023Quote: From the H6-RapA expression plasmid and pSNAP-tag(T7) plasmid (NEB #101137), we constructed a plasmid pKI1 (Addgene #199118 ...
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bioRxiv - Plant Biology 2024Quote: ... 0.4 μL Buffer Tag DNA polymerase (5 U/μL, NEB, Cat. No. B9004S), 1.6 μL dNTP (TAKARA ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB) by deleting the amino acids (number 169 –181 ...
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bioRxiv - Molecular Biology 2019Quote: ... coli strains BL21-CodonPlus-RP or Lemo21(DE3, New England Biolabs). Bacterial lysates were prepared as previously described(48 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli NEB Stbl competent cells (New England Biolabs, Ipswich, MA, USA) and sequenced with U6 promoter primer listed in Supplementary Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... Escherichia coli competent cells NEB 5-alpha (C2987, New England Biolabs), NEB Stable (C3040 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli K-12 DH10B (Neb 10-beta, NEB catalogue no. C3019H), E ...
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bioRxiv - Bioengineering 2020Quote: Escherichia coli NEB5α high efficiency competent cells were obtained from NEB for plasmid construction ...
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bioRxiv - Microbiology 2020Quote: ... All cloned constructs were transformed into SHuffleT7 Competent Escherichia coli (NEB), and expression was induced with 1 mM IPTG (isopropyl-D-thiogalactopyranoside) ...
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bioRxiv - Systems Biology 2021Quote: ... coli clones using the Monarch Plasmid Miniprep Kit (New England BioLabs).
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bioRxiv - Molecular Biology 2020Quote: ... we used 5 units of Escherichia coli RNase HII (NEB, M0288L) in place of the C ...
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bioRxiv - Microbiology 2019Quote: ... coli poly(A) polymerase (New England BioLabs, Beverly, MA, USA; M0276S).
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, C3013). The cells were grown in LB medium containing 50 μg/ml kanamycin at 37°C until OD600 nm 0.5 ...
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bioRxiv - Microbiology 2021Quote: ... coli using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) following manufacturers’ instructions ...
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bioRxiv - Bioengineering 2019Quote: Escherichia coli NEB5α high efficiency competent cells were purchased from NEB for plasmid construction ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) were purchased as competent cells (New England Biolabs). E ...
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bioRxiv - Cell Biology 2021Quote: ... coli (BL21/pLysS) carrying the expression constructs using amylose beads (NEB) and S-glutathione beads (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... coli poly-A polymerase (New England Biolabs, NEB, Ipswich, MA, USA) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... coli poly-A polymerase (New England Biolabs, NEB, Ipswich, MA, USA) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain BL21 (DE3) and subsequently purified with amylose beads (NEB). The RNA affinity purification of mRNA 3’ processing complex was carried out essentially as described before (15) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia coli strains NEB Turbo and NEB Express (New England BioLabs) were used for cloning ...
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bioRxiv - Biochemistry 2023Quote: ... coli DH5α and NEB10ß cells (New England Biolabs GmbH, Frankfurt, Germany) were used.
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bioRxiv - Bioengineering 2023Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning purposes ...
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bioRxiv - Immunology 2023Quote: ... for transformation with competent Escherichia coli NEB5a bacteria (New England Biolabs) and colony selection ...
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
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bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
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bioRxiv - Microbiology 2021Quote: ... The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S) and run on a 1.5 % agarose gel ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs) ...
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bioRxiv - Genetics 2019Quote: ... These were used in a PCR reaction with Q5 Hi-Fidelity polymerase (NEB, USA) in combination with the pLuc-GAL5.1 reporter construct previously described 8 as template to produce pLuc-GALΔEGR ...
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bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...