Labshake search
Citations for New England Biolabs :
1851 - 1900 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... Endogenous proteins were captured onto protein G-magnetic beads (NEB; #S1430S), washed extensively in IP buffer and used for POT1wtand POT1V29L source ...
-
bioRxiv - Microbiology 2020Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2020Quote: Sec24D was subcloned from pEGFP Sec24D into pcDNA3.1 with an N-terminal myc-6xHis tag using Gibson Assembly (E5510, New England Biolabs). pcDNA3.1 was linearized with Not1 (R3189 ...
-
bioRxiv - Microbiology 2021Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
-
bioRxiv - Genomics 2022Quote: ... Two nested single-tail adapter/tag (STAT)-PCR [29] were performed with LongAmp Hot Start Taq polymerase (NEB, M0533S) using adaptor binding P5_1 and P5_2 primers and corresponding insert primer pairs (see Supplementary Table 2) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were labelled with the cleavable SNAP-tag probe BG-S-S-549 (a gift from New England Biolabs) in complete medium for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: The fluorescent SNAP-tag substrate BG-CF640R was synthesized by amidization reaction of BG-NH2 (New England Biolabs #S9148S) and CF640R-NHS ester (Biotium #92108) ...
-
bioRxiv - Microbiology 2021Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... a Hisx6 sequence tag was inserted into the BamH1 and Xho1 sites of MCS2 of pSNAPf (New England Biolabs) to create pSNAPf_Hisx6 ...
-
bioRxiv - Molecular Biology 2020Quote: ... pET28a-6xHis-MBP-Tev cleavage site-mGFP-FUS-TIS-Strep-tag II was transformed into BL21 E.coli (New England Biolabs). Two fresh colonies were cultivated overnight in 2 × 50 ml SOB medium at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... a Hisx6 sequence tag was inserted into the BamH1 and Xho1 sites of MCS2 of pSNAPf (New England Biolabs) to create pSNAPf_Hisx6 ...
-
bioRxiv - Biophysics 2023Quote: ... into a pSGEM construct with five consecutive repeats of the myc tag (Lörinczi et al., 2015) using NcoI and HindIII (New England Biolabs).
-
bioRxiv - Biochemistry 2023Quote: ... the HA-epitope was replaced by a 6xHis tag using the Q5 Site Directed Mutagenesis Kit (New England Biolabs) to generate Ptch1 KR-His ...
-
bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were incubated in dye solution (1 μM SNAP-tag ligand BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no ...
-
bioRxiv - Biophysics 2023Quote: ... cells were incubated in dye solution (1 μM SNAP-tag ligand BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM dithiothreitol (catalog no ...
-
bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
-
bioRxiv - Zoology 2023Quote: ... One μg of total RNA was reverse transcribed in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... the constructed plasmids were transformed by heat shock into chemically competent Top10 Escherichia coli (New England BioLabs). Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1 ...
-
bioRxiv - Bioengineering 2019Quote: ... Chemically-competent Escherichia coli DH10B cells were used for all routine cloning (New England Biolabs, Ipwich, MA). The E ...
-
bioRxiv - Cancer Biology 2022Quote: ... was amplified using NEB Stable competent Escherichia coli (high efficiency) (C3040H, NEW ENGLAND BioLabs, Ipswich, MA, USA). The full-length ALKBH5 sequence (NM_017758) ...
-
bioRxiv - Neuroscience 2019Quote: ... and used to transform NEB 5-α competent Escherichia coli cells (New England Biolabs, Whitby, ON, Canada). After screening plasmid constructs for directionality using T7 promoter oligonucleotide and gene-specific primers (Table S1) ...
-
bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
-
bioRxiv - Immunology 2022Quote: ... coli (C3019H/C3019I) was transformed with a plasmid DNA using High Efficiency Transformation Protocol (New England Biolabs). After picking a single colony ...
-
bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
-
bioRxiv - Bioengineering 2023Quote: ... The sequence-confirmed plasmid was transformed into Escherichia coli strain BL21(DE3) chemically competent cells (NEB C2527) according to NEB’s transformation protocol and plated on LB plates containing 100 µg/ml carbenicillin.
-
bioRxiv - Bioengineering 2022Quote: ... The cloned plasmids were sequence verified using Sanger sequencing and transformed into T7-Express Escherichia coli (NEB) via heat-shock.
-
bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Libraries were amplified for 12 PCR cycles with unique dual index primers using the NEBNext Hi-Fi 2X PCR Master Mix (New England Biolabs). Amplified libraries were purified using a 1.2X ratio of Axygen magnetic beads (Corning Inc ...
-
bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
-
bioRxiv - Biophysics 2021Quote: Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB). For Immobilized His6-tagged MukB ...
-
bioRxiv - Biochemistry 2020Quote: ... Vectors containing cohesin trimers were generated by combining pACEbac1 SMC1-His SMC3 with pIDC SCC1-2xStrepII by a Cre recombinase reaction (New England Biolabs). The vector for the Scc2/4 expression was also created by combining the pACEbac1 SCC2-2xStrepII with pIDC His-SCC4 using Cre recombinase.
-
bioRxiv - Immunology 2021Quote: ... Restriction digest of pMK-RQ plasmid DNA containing EtIMP1 was performed using Bam HI and Not I restriction enzymes (New England Biolabs). Restriction digest of pYD1-EtAMA1Cit plasmid was performed with the same restriction enzymes to remove the EtAMA1 insert but retain the citrine tag ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tagmented DNA was amplified with 12 cycles of PCR using the NEBNext Hi-Fi 2X PCR Master Mix (NEB M0541) and unique dual index primers ...
-
bioRxiv - Cell Biology 2021Quote: ... coding sequence was amplified from Arabidopsis cDNA and assembled into a pGreen0179-35S-spFLA11-His-YFP vector using NEBuilder HiFi DNA Assembly kit (NEW ENGLAND Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... was further amplified by PCR with an overlapping sequence with pGEX-6P and cloned immediately after the PreScission site of the pGEX-6P vector by Hi-Fi DNA assembly (NEB).
-
bioRxiv - Biophysics 2022Quote: ... with the (G4S)4-GGS linker was amplified by PCR by adding a linker sequence and inserted into the pGEX-6P vector by Hi-Fi DNA assembly (NEB).
-
bioRxiv - Plant Biology 2021Quote: ... containing the protospacer sequence and sequences that overlap with pMH-Cas9 were assembled into pMH-Cas9 using Hi-Fi DNA Assembly Mastermix (NEB). The Hi-Fi reaction was transformed into E ...
-
bioRxiv - Cell Biology 2021Quote: ... GST aPKC PBM and his aPKC kinase domain-PBM (residues 259-606) were cloned as previously described (10) using Gibson cloning (New England BioLabs), Q5 mutagenesis (New England BioLabs ...
-
bioRxiv - Genetics 2019Quote: ... The donor sequence was generated by subcloning 596 bp upstream of the hrpk-1 stop codon and 600 bp downstream of hrpk-1 stop codon into the pDD282 vector [45] using Hi Fi assembly kit (NEB). The hrpk-1 stop codon was eliminated from the donor sequence to allow in frame GFP tag addition ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Insert NGRP or scFv13R4 was assembled into backbone pDEST-ORS-PelB TMT following NEB builder Hi-fi mix protocol (NEB) to create pDEST-ORS-NGRP/scFv13R4 TMT ...
-
bioRxiv - Cell Biology 2022Quote: ... EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs) as described by the manufacturer.
-
bioRxiv - Cell Biology 2021Quote: ... and ligated to linearized vector pGex-6p-1 (GST) or pET28-SUMO (His) also cut with the same restriction enzymes using T4 DNA ligase (NEB).
-
bioRxiv - Molecular Biology 2022Quote: ... The Hi-C library for Illumina sequencing was prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... a tolerant position was mutated to facilitate making a labeled LexA variant.56 The His-LexA W201C mutant was generated using a Q5 Site-Directed Mutagenesis Kit (NEB) and the mutation was confirmed via sequencing ...
-
bioRxiv - Cell Biology 2022Quote: ... The resultant adaptor-ligated Hi-C library was amplified by PCR with five to seven cycles with Q5 master mix (M0544, NEB) and index primers (E7335/E7500/E7710/E7730 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ebony non-coding regions from different species were amplified via PCR and cloned into the S3AG vector using NEBuilder Hi-Fi DNA assembly (NEB) (Table X) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.