Labshake search
Citations for New England Biolabs :
1801 - 1850 of 2894 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The cleaved 6xHis-MBP tag was removed by incubating the solution with Ni-NTA magnetic beads (NEB; S1423) for 1 hour at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
-
bioRxiv - Plant Biology 2021Quote: ... The glutathione S-transferase (GST) tag was cleaved using Factor Xa Protease (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s instruction.
-
bioRxiv - Biophysics 2021Quote: ... The SNAP tag on Nup358min-zip and Nup358min was biotinylated with SNAP-biotin substrate (New England BioLabs, MA) as described99.
-
bioRxiv - Biophysics 2019Quote: ... Purity and molecular weight were confirmed by staining with a SNAP-tag-reactive benzylguanine-Alexa 647 dye (NEB) and running the proteins on a 4-20% NuPAGE Bis-Tris gel ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM CaCl2 and the TRX-6His-S-tag was cleaved overnight at RT with enterokinase protease (NEB). The ET domain was further purified using Nickel-NTA resin (Thermo Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
-
bioRxiv - Cell Biology 2022Quote: ... the HA tag and the Bub3 genomic fragment were inserted into pPEE37 (James Fraser) using Gibson assembly (NEB). Expression of HA-Bub3 was confirmed by immunoblotting with anti-HA antibodies ...
-
bioRxiv - Synthetic Biology 2023Quote: ... HDR enhancing factors and MS2 tag were cloned between Esp3I sites by Golden Gate Assembly (New England Biolabs). All the primers are listed in Supplementary Table 2 ...
-
bioRxiv - Biochemistry 2022Quote: ... a Thioredoxin-tag (Trx) and a TEV cleavage site via restriction digestion (NcoI/XhoI, New England BioLabs®) and subsequent ligation (T4 DNA Ligase ...
-
bioRxiv - Plant Biology 2023Quote: ... AtPAXXL228E fused to hexa-histidine followed by a GST tag (pGAT3-atpaxxL228E) was expressed in BL21(DE3) (NEB) and purified using a 5 ml GSTrap HP column (Cytiva) ...
-
bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
-
bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
-
bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... MWCO 10,000 filters and then digested with 10 µl Tobacco etch virus nuclear-inclusion-a endopeptidase (TEV) (New England Biolabs, Ipswich, MA) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
-
bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... The ligated product was then chemically transformed into Escherichia coli DH5-α competent cells (NEB, Australia) following the company’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
-
bioRxiv - Microbiology 2020Quote: ... coli total RNA was purified using the Monarch® Total RNA Miniprep Kit (New England Biolabs) according to manufacturer’s instructions including the recommended on-column DNase treatment.
-
bioRxiv - Genomics 2022Quote: The pTXB1-nbTn5 vector was transformed into BL21(DE3)-competent Escherichia coli cells (NEB, no. C2527), and nb-Tn5 was produced via intein purification with an affinity chitin-binding tag (Picelli et al ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and the ligation mix was directly used to transform 10-beta competent Escherichia coli cells (NEB), to amplify pSG01 ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli BL21 (DE3) strain and purified with amylose agarose beads (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and biosensor screens were all performed in Escherichia coli DH10B (New England BioLabs, Ipswich, MA, USA). Growth media consisted of LB-Miller (LB ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Golden gate (Type-IIS) cloning was conducted using chemically competent Escherichia coli DH5α cells (NEB, C2987H). Construction of destination vectors with the toxic selection marker ccdB was done with chemically competent One Shot ccdB Survival 2 T1R E ...
-
bioRxiv - Genomics 2023Quote: The library was expanded by transformation into chemically competent dam-/dcm- competent Escherichia coli cells (NEB). For every 100 μL of competent E ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli were used for cloning plasmids smaller than 10 kb and NEBStable (NEB cat no. C3040I) competent E ...
-
bioRxiv - Plant Biology 2023Quote: ... The solution was then used to transform NEB turbo Escherichia coli competent cells (NEB, MA, USA). pCambia1300/SPRI1A-C+12 was created in our previous study (Fujii et al. ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
-
bioRxiv - Developmental Biology 2023Quote: ... were amplified in NEB® Stable Competent Escherichia coli (High Efficiency) cells (New England BioLabs, #C3040), purified using NucleoSpin Plasmid kit (MACHENREY-NAGEL ...
-
bioRxiv - Cell Biology 2023Quote: ... coli and synthesized (Eurofins Genomics) and cloned into the pMAL-c6T vector (New England Biolabs, N0378). Protein expression in the E ...
-
bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
-
bioRxiv - Biochemistry 2024Quote: ... lentiviral plasmids were constructed using a pLX317 backbone in competent Escherichia coli (New England Biolabs, C3050H). Lenti-XTM 293T (Takara ...
-
bioRxiv - Biochemistry 2021Quote: LCB1 was synthesized and cloned into E.coli using a pET expression vector via Hi-Fi DNA assembly (NEB; E2621S) with a gBlock (ITD ...
-
bioRxiv - Immunology 2021Quote: ... Restriction digest of EtMIC3 PCR product was performed using Bam HI and Eco RI restriction enzymes (New England Biolabs) to extract antigen coding sequence for cloning into pYD1 plasmid vector ...
-
bioRxiv - Cell Biology 2021Quote: ... Par-3 PDZ1-APM and PDZ1-APMΔPDZ2 were first his-purified (described above) prior to incubation with amylose resin (NEB). Amylose-bound Par-3 was then washed and resuspended in binding buffer as described for GST proteins.
-
bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Near full-length 16S rRNA genes were amplified by PCR using Phusion Hi-Fidelity DNA Polymerase (New England BioLabs) and a bacteria-specific primer set ...
-
bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
-
bioRxiv - Genomics 2023Quote: Hi-C single-index library preparation of MCF7 cells was performed as previously described using MboI (New England Biolabs) restriction enzyme (30).
-
bioRxiv - Microbiology 2023Quote: ... Gibson assemblies were completed using a 2x HI-FI Assembly master mix following the manufacturer’s protocol (New England Biolabs). PCR clean-up was done using the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
-
bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
-
bioRxiv - Biochemistry 2020Quote: ... pET-SUMO and pMALX-E were digested with Nde1/BamHI or Nhe1/ HindIII (New England Biolabs, Frankfurt, Germany), respectively ...
-
bioRxiv - Cell Biology 2020Quote: ... E-Syt3ΔC2C was produced by introducing a 2021_2257 deletion using the Q5 site-directed mutagenesis kit (NEN Biolabs). The deletion created a reading frameshift and a premature stop that removed the C2C domain and created a 707 aa long protein with a 35 aa C-neoextension ...
-
bioRxiv - Cell Biology 2022Quote: ... Genomic sequence of isolated clones at the target locus was further confirmed by PCR cloning (E#1202S, NEB) and Sanger sequencing (Australian Genome Research Facility ...
-
bioRxiv - Microbiology 2022Quote: ... Protein samples were then mixed with Blue Protein Loading Dye (NEB) boiled for 10 minutes and separated on 10% SDS-PAGE gel for 50 minutes at 140 V ...
-
bioRxiv - Plant Biology 2021Quote: ... and extracted nuclear proteins were treated with lambda protein phosphatase (NEB) according to the manufacturer’s instruction.