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Citations for New England Biolabs :
1651 - 1700 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and digested overnight at 37°C with 1 Unit of NlaIII enzyme (R0125L, NEB)/μg of DNA (under agitation-600 rpm) ...
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bioRxiv - Molecular Biology 2019Quote: ... About 1 µg of purified RNA template was transcribed by T7 RNA polymerase (NEB) and the integrity of the RNAs was examined by agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... The reannealed fragments were treated with 5 units of T7 endonuclease 1 (NEB, #M0302S) at 37°C for 25 min and immediately separated with 7% polyacrylamide gel electrophoresis.
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Genomics 2020Quote: ... PCR products were treated with 1 μl of exonuclease (New England Biolabs, 20 units), and the mixture was then incubated at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was circularised by incubation with 10U T4 RNA Ligase 1 (NEB, USA) in 20 μL of the reaction mixture containing 1x T4 RNA Ligase Buffer (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... PCR 1 conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Genomics 2021Quote: ... The template was destroyed by adding 1 μL 20,000 U/mL DpnI (NEB, R0176S) to the mix and incubating at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S). Additional AMPure clean-ups at the start and the end of library preparation were included ...
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bioRxiv - Neuroscience 2019Quote: ... the reannealed PCR products were digested with T7 endonuclease 1 (T7E1, New England Biolabs). Digestion was performed using 10 unites of T7E1 enzyme and incubated for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Plant Biology 2021Quote: ... and ligated at a 3:1 amplicon:plasmid ratio with t4 DNA ligase (NEB, US) overnight at 16°C ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed by incubation with 1 µl of RNase H (NEB, USA) for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... then directly resuspended in 1× SDS (sodium dodecyl sulphate) sample buffer (New England BioLabs) per 1 ml of OD600 of 1.0 cells in 200 μl sample buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μL PCR products were incubated with 1 μL DpnI enzyme (New England Biolabs) to clean up templates at 37 °C for 2.5 hours and then purified by gel-extraction following the manufacturer instructions (Cat# D2500-02 ...
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bioRxiv - Genomics 2022Quote: 500 ng to 1 μg gDNA were cleaved by SacI-HF (New England Biolabs) for 15 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA (Omicron-BA.1; EPI_ISL_7062525) by LunaScript RT SuperMix (NEB). PCR reactions using Q5® High-Fidelity DNA Polymerase were performed with the primers and templates described in Supplementary Information Table 3 ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of 10 mM dNTP (cat. n° N0447L, NEB, New England Biolabs, USA), 0.2 µL of RNase Inhibitor 40 U / µL (cat ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of 10 mM dNTP (cat. n° N0447L, NEB, New England Biolabs, USA), 0.2 µL of RNase Inhibitor 40 U / µL (cat ...
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bioRxiv - Cancer Biology 2020Quote: Specific antibodies against Slug (C19G7, Cell Signaling Technology, NEB, Frankfurt, Germany, #9585, 1:400), pan-cytokeratine (polyclonal ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight at 37°C with MSPI (20U μl-1 NEB cat: R0106L) and 10x NEBuffer2 (NEB cat ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ adaptor ligation using T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Generation of cDNA was done by using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, M0242, 200,000 units/ml) and incubated at 16°C for 14 h ...
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bioRxiv - Microbiology 2022Quote: Fragmented gDNA (1 μg) was end-repaired using an End-repair module (NEB#E6050), and dA-tailed using Klenow (NEB#6053) ...
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bioRxiv - Molecular Biology 2019Quote: ... Ligation reaction proceeded by addition of T4 RNA Ligase 1 (New England Biolabs, M0437M) at 23°C for 75 minutes ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: The Cry2(1-531) gene was amplified by polymerase chain reaction (NEB Q5 polymerase) from the full length Cryptochrome-2 gene using a forward primer encoding an XhoI-restriction site with the sequence ...
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bioRxiv - Genomics 2019Quote: ... 1 × SYBR Green and 5μl NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541S), to estimate the optimum number of enrichment cycles with the following program ...
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bioRxiv - Genetics 2020Quote: ... remaining ssDNA oligonucleotides were digested by the addition of 3 μL exonuclease 1 (NEB) to 20 μL extracted DNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... using 1 µL of each sample or mixed with 1x DNA loading dye (NEB) and loaded on a 10 % native polyacrylamide gel (PAGE ...
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bioRxiv - Synthetic Biology 2020Quote: ... we cooled the samples to 37C and added 20U of Exonuclease 1 (NEB, M0293S) to the reaction to remove excess primers ...
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bioRxiv - Microbiology 2019Quote: ... Incubation for 1 h at 55°C with 8U of proteinase K (NEB P8107S) finally disrupted viral capsids ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehybridized product was digested with 1 μL T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 hour and separated on a 1.5% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting PCR amplicons were denatured and re-annealed in 1 × NEB buffer 2 (NEB) in a total volume of 9 μl using a following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented RNA was then purified and circularized with T4 RNA ligase 1 (NEB). Tandem repeats were generated during first-strand synthesis at 42 °C for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Synthetic Biology 2019Quote: All digestions were performed for 1-2h at 37° with REs purchased from NEB, and purified using the Monarch PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... an RNA-protein complex was formed with 1 µM Cas9 protein (New England Biolabs) and 1 µM gRNA pool (3 gRNAs in total ...
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bioRxiv - Genomics 2019Quote: ... in 1× Buffer 3 (Bionano Genomics) or 120 U of Nb.BssSI (New England Biolabs) in 1× NEBuffer 3.1 ...
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bioRxiv - Genetics 2020Quote: ... and 0.3 μL quick ligase in 1 x quick ligase buffer (New England Biolabs) with a total volume of 6 μL ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
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bioRxiv - Biochemistry 2021Quote: ... one of which was incubated with 1 µl nicking enzyme (10 units Nt.BspQI (NEB) or 5 units Nb.Bpu101 (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The chambers of the microplate were pre-treated with 1 mg/ml BSA (NEB) for 30 min before aspirating the BSA ...
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bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Digestion was performed for 1 h using Cut Smart Buffer (NEB, Cat no. B7204S). Next ...