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Citations for New England Biolabs :
1851 - 1900 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL−1, New England Biolabs, Ispwich, USA) was added per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then probed with the primary anti-MBP antibody (NEB E8032S, 1:10000) diluted in 5% NFDM (overnight ...
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bioRxiv - Biochemistry 2023Quote: Extracted samples (1 μL) were digested with NotI-HF (0.89 unit/μL, New England Biolabs) and ClaI (0.44 unit/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the isolated RNA was treated with 1 μl (5 units) RppH enzyme (New England Biolabs) in NEB Buffer 2 (50 mM NaCl ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Physiology 2023Quote: ... 1 U of Phusion® Hot Start II High-Fidelity DNA polymerase (New England BioLabs), 500 nM of the 27F-DegS primer ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 in the presence of 25 mU ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand ...
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bioRxiv - Biochemistry 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to the annealed product ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added and incubated at 25 °C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Plugs were rinsed with TE and then washed with 1 ml NEB buffer 4 (NEB) 3 × 15min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of T4 DNA ligase high-concentration (2,000,000, units/mL, NEB, cat. no. M0202T) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
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bioRxiv - Evolutionary Biology 2024Quote: ... adding 3.5 µL of Cutsmart buffer and 1 µL of ApaLI restriction enzyme (NEB #R0507L) to each sample’s Step 2 PCR product ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
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bioRxiv - Biochemistry 2024Quote: ... In short 5’-phosphorylated and 3’-OH RNA was circularized with RNA ligase 1 (NEB) followed by denaturing PAGE purification as described above ...
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bioRxiv - Biochemistry 2024Quote: ... EU-labeled cells fixed and permeabilized as above were incubated in 1×CutSmart buffer (NEB) with 4ng/µL RNase A or 5U/µL benzonase ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... the target site was amplified by Taq DNA polymerase:DeepVent polymerase 200:1 U (both NEB) with CRISPR efficiency primers and sequenced ...
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bioRxiv - Genomics 2024Quote: ... amplifications were submitted to cleavage assays using the T7 Endonuclease 1 (T7E1) enzyme protocol (NEB). PCR products of confirmed G2 heterozygous (n = 48 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were ethanol-fixed and incubated with 1:100 anti-H3K27me3 antibody (NEB, 9733S) as described previously (34) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
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bioRxiv - Neuroscience 2024Quote: ... 2 ml of virus was incubated with 1 ml DNase I (NEB, 2 units/ml), 4 µL DNase I buffer ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this was then combined with 5 μL Q5 buffer (NEB, cat#M0491 S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5’ adaptor (rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCr-GrArCrGrArUrCrNrNrNrCrGrArNrNrNrUrArCrNrNrN, N is for a random nucleotide) by T4 ligase 1(NEB) sequentially ...
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bioRxiv - Genomics 2022Quote: ... The electroporated bacteria was immediately split into ten 1 mL aliquots of outgrowth medium (NEB, B9035S) and incubated at 37°C for 1 hr then independently scaled up in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (Teknova ...
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bioRxiv - Genomics 2020Quote: ... and barcoded with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs). We sequenced prepared libraries on an Illumina MiSeq using MiSeq Reagent Kit v3 (2×300 cycles ...
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Molecular Biology 2019Quote: ... The Cap 1 25mer RNA was purified using Monarch RNA Cleanup kit (50 μg capacity, NEB). Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Bioengineering 2019Quote: ... The inserts were ligated to the phPB52 digested vector in 1× T4 DNA ligation buffer (NEB) with 30 ng of vector DNA with three molar excess of synthetic inserts ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl Exonuclease I and 2.44 μl Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2019Quote: ... 600 ng of total RNA were mixed with 0.83 μL 100 mM tris pH 7.5 and 0.17 μL 3 mg·mL−1 Random Primers (NEB) to a volume of 5.25 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genomics 2019Quote: ... Ligation was then performed in a ratio of 1:50 (vector:insert) with T4 DNA ligase (NEB) at room temperature for one hour and then transformed into NEB10 competent cells (NEB) ...
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bioRxiv - Genetics 2019Quote: ... 1 uL of cDNA or gDNA was amplified using Q5 High Fidelity 2X Master Mix (NEB). An indexing PCR was performed next using Nextera XT index kit primers (Illumina ...
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bioRxiv - Genomics 2021Quote: ... 500 ng of DNA was dissolved in 9 μL of 1 × CutSmart buffer (New England Biolabs) and sheared by pipetting 30 times with a pipette set at 8 μL with a P2/P10 tip ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...