Labshake search
Citations for New England Biolabs :
1451 - 1500 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB, #E6440G), followed by 2×150 bp sequencing on the Illumina MiSeq ...
-
bioRxiv - Cell Biology 2023Quote: ... Kinase reactions were performed with 1 μL commercial CK1δ (New England Biolabs) in 1x PMP buffer supplemented with 100 μM unlabeled ATP ...
-
bioRxiv - Neuroscience 2023Quote: ... The PCR product was digested with 1 unit of MwoI (NEB #R0573S) for 2 hrs at 60°C ...
-
bioRxiv - Microbiology 2023Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs) (0.01 units/μL) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 1 μL of 10 mM dNTP mix (NEB N0447S, Ipswich, MA). This initial premix was heated at 65°C for 5 minutes ...
-
bioRxiv - Genomics 2023Quote: ... 1 μl 10 mM mix of dGTP and dTTP (NEB #N0442S, #N0443S), 5 μl 10x T4 DNA Ligase Buffer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB, 10x). We found that using these enzymes resulted in higher efficiency and fidelity for the cloning of mismatched gRNA libraries ...
-
bioRxiv - Cell Biology 2024Quote: ... The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB) by incubating at 25 °C for 16h ...
-
bioRxiv - Microbiology 2023Quote: ... The NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, USA) was used for multiplexing ...
-
bioRxiv - Biophysics 2023Quote: ... 1 μg of the biotinylated lambda DNA is treated with Nt.BspQI (NEB) for 1 hour at 50°C then heat inactivated for 20 minutes at 80°C ...
-
bioRxiv - Biophysics 2023Quote: ... and dGTP were incubated and 10 units of DNA polymerase 1 (NEB) for 6 minutes at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
-
bioRxiv - Bioengineering 2023Quote: ... 0.4 µL Antarctic Thermolabile UDG (1 U/µL; New England Biolabs, M0372S), 5.4 µL Bst 2.0 DNA Polymerase (120 U/µL ...
-
bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
-
bioRxiv - Cell Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB E7335) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... beads were incubated with 0.5 U/ μl T4 RNA ligase 1 (NEB) in 1x ligase buffer (containing 1 mM ATP ...
-
bioRxiv - Molecular Biology 2023Quote: Unlabeled purified RNA targets were dephosphorylated using 1 U antarctic phosphatase (NEB) incubated with 1X of provided buffer during 20 min at 37°C and then 5 min at 65°C to inactivate the enzyme ...
-
bioRxiv - Biophysics 2024Quote: ... 1 μL of 5U/ μL Klenow Fragment of DNA polymerase I (NEB) and 2 μL of 10 mM dNTP mix (SERVA ...
-
bioRxiv - Genomics 2024Quote: ... 10 µg of RNA was treated with 1 µL Xrn1 (NEB, M0338S) for 1 h at 37 °C to remove decapped ...
-
bioRxiv - Neuroscience 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB). All libraries were sequenced 150bp paired end using an Illumina Novaseq 6000 with 15 million reads on average per sample.
-
bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA) and passed through an amylose resin column (NEB#E8021L). Columns were washed prior to recombinant protein elution with 10 mM maltose in column buffer.
-
bioRxiv - Genomics 2024Quote: ... 1% SDS) and 20 μl of Proteinase K (NEB, cat. no. P8107S). Subsequently ...
-
bioRxiv - Bioengineering 2024Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
-
bioRxiv - Microbiology 2024Quote: Cell lysates were digested for 1 h with Endo H (P0702, NEB) or PNGase F (P0704 ...
-
bioRxiv - Biochemistry 2024Quote: ... coli exonuclease III (Xth) was performed in NEBuffer 1 (New England Biolabs) with 1 nM enzyme ...
-
bioRxiv - Biochemistry 2024Quote: ... either with or without 1 µL of PNGase F (500 Units, NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... for 1 hour at 25°C with 20% PEG8000 (New England Biolabs). After ligation ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.05% SDS) supplemented with 5.25 µl of 20lJmglJml−1 Proteinase K (NEB) and 1.25 µL of 1lJM DTT (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... DNA remaining in the RNA samples was removed by DNase 1 (NEB) digestion ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB: E7335L). Samples with total RNA concentration of >10 ng/µl (0.5 µg total amount ...
-
bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was treated with 5 units of T7 endonuclease 1 (New England Biolabs) for 20 min at 37 °C and analyzed by 2% agarose gel electrophoresis ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 1 μL of 10 mM dNTPs (New England Biolabs, Ipswich, MA, USA) to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs, Ipswich, USA), 1 µl of BsaI-HFV2 (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL Bst 2.0 WarmStart® DNA polymerase (0.32 u; Biolabs, Ipswich, MA), 1 μL Hydroxy Naphthol Blue (HNB ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was phosphorylated for 1 h at 37oC with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
-
bioRxiv - Cell Biology 2022Quote: ... 1/10 T7 RNA polymerase mix (HighScribe T7 High Yield RNA synthesis NEB)) at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... 1 or 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) and nuclease-free water to made up to 10 μl ...
-
bioRxiv - Genomics 2019Quote: ... Bead-nuclei were subsequently washed with 200 µL 1× RNA ligase buffer (NEB) supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB ...
-
bioRxiv - Genomics 2019Quote: ... and resuspended in 95 µL PNK solution (1× T4 DNA ligase buffer (NEB), 0.1% Triton X-100) ...
-
bioRxiv - Microbiology 2021Quote: ... 10 μM dNTPs (0.5 μl per reaction) in 1× Klenow reaction buffer (NEB). Next ...
-
bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... and 1 µL used in PCR with Taq DNA Polymerase (New England Biolabs). Primers amplified the repB region of plasmids pAM714 and pAM771 (pAD1 repB-For ...
-
bioRxiv - Neuroscience 2019Quote: ... 1 µM NGFR121W-SNAP was coupled with 3 µM BG549 surface (NEB # S9112) for 1 hour at 37°C in calcium imaging (CIB ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 mM EGTA and 2 mM Vanadyl Ribonucleoside complex (VRC, New England Biolabs), washed three times with 70% ice-cold ethanol and kept at −20°C.
-
bioRxiv - Microbiology 2019Quote: ... 1 µl of 200 mM dNTPs (New England Biolabs Inc., Ipswich, MA, USA), 2 µl of 5 µM of each Primer ...
-
bioRxiv - Microbiology 2019Quote: ... The Quick-Load 1 kb Extend DNA Ladder (New England BioLabs, Ipswich, MA) was used as a molecular marker.
-
bioRxiv - Genetics 2019Quote: ... and then treated with 5 units of T7 endonuclease 1 (New England Biolabs) for 30 min at 37°C and finally analyzed by 2% agarose gel electrophoresis.